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Viability of African Swine Fever Virus with the Shallow Burial with Carbon Carcass Disposal Method

African swine fever (ASF) is a highly contagious swine disease with high mortality. In many countries, culling pigs infected and exposed to the ASF virus is mandatory to control the disease, which poses a real challenge in the disposal of large numbers of carcasses during ASF outbreaks. Shallow buri...

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Autores principales: Duc, Hoang Minh, Hutchinson, Mark, Flory, Gary A., Ngan, Pham Hong, Son, Hoang Minh, Hung, Le Van, Hoa, Tran Thi Khanh, Lan, Nguyen Thi, Lam, Truong Quang, Rozeboom, Dale, Remmenga, Marta D., Vuolo, Matthew, Miknis, Robert, Burns, Amira, Flory, Renée
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140975/
https://www.ncbi.nlm.nih.gov/pubmed/37111514
http://dx.doi.org/10.3390/pathogens12040628
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author Duc, Hoang Minh
Hutchinson, Mark
Flory, Gary A.
Ngan, Pham Hong
Son, Hoang Minh
Hung, Le Van
Hoa, Tran Thi Khanh
Lan, Nguyen Thi
Lam, Truong Quang
Rozeboom, Dale
Remmenga, Marta D.
Vuolo, Matthew
Miknis, Robert
Burns, Amira
Flory, Renée
author_facet Duc, Hoang Minh
Hutchinson, Mark
Flory, Gary A.
Ngan, Pham Hong
Son, Hoang Minh
Hung, Le Van
Hoa, Tran Thi Khanh
Lan, Nguyen Thi
Lam, Truong Quang
Rozeboom, Dale
Remmenga, Marta D.
Vuolo, Matthew
Miknis, Robert
Burns, Amira
Flory, Renée
author_sort Duc, Hoang Minh
collection PubMed
description African swine fever (ASF) is a highly contagious swine disease with high mortality. In many countries, culling pigs infected and exposed to the ASF virus is mandatory to control the disease, which poses a real challenge in the disposal of large numbers of carcasses during ASF outbreaks. Shallow burial with carbon (SBC) Thanks ew mortality disposal method developed from deep burial and composting. The present study investigates the effectiveness of SBC in disposing of ASF virus-infected pigs. The real-time PCR results showed that DNA of the ASF virus was still detected in bone marrow samples on day 56, while the virus isolation test revealed that the infectious ASF virus was destroyed in both spleen and bone marrow samples on day 5. Interestingly, decomposition was found to occur rapidly in these shallow burial pits. On day 144, only large bones were found in the burial pit. In general, the results of this study indicated that SBC is a potential method for the disposal of ASF-infected carcasses; however, further studies are needed to provide more scientific evidence for the efficacy of SBC in different environment conditions.
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spelling pubmed-101409752023-04-29 Viability of African Swine Fever Virus with the Shallow Burial with Carbon Carcass Disposal Method Duc, Hoang Minh Hutchinson, Mark Flory, Gary A. Ngan, Pham Hong Son, Hoang Minh Hung, Le Van Hoa, Tran Thi Khanh Lan, Nguyen Thi Lam, Truong Quang Rozeboom, Dale Remmenga, Marta D. Vuolo, Matthew Miknis, Robert Burns, Amira Flory, Renée Pathogens Article African swine fever (ASF) is a highly contagious swine disease with high mortality. In many countries, culling pigs infected and exposed to the ASF virus is mandatory to control the disease, which poses a real challenge in the disposal of large numbers of carcasses during ASF outbreaks. Shallow burial with carbon (SBC) Thanks ew mortality disposal method developed from deep burial and composting. The present study investigates the effectiveness of SBC in disposing of ASF virus-infected pigs. The real-time PCR results showed that DNA of the ASF virus was still detected in bone marrow samples on day 56, while the virus isolation test revealed that the infectious ASF virus was destroyed in both spleen and bone marrow samples on day 5. Interestingly, decomposition was found to occur rapidly in these shallow burial pits. On day 144, only large bones were found in the burial pit. In general, the results of this study indicated that SBC is a potential method for the disposal of ASF-infected carcasses; however, further studies are needed to provide more scientific evidence for the efficacy of SBC in different environment conditions. MDPI 2023-04-21 /pmc/articles/PMC10140975/ /pubmed/37111514 http://dx.doi.org/10.3390/pathogens12040628 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Duc, Hoang Minh
Hutchinson, Mark
Flory, Gary A.
Ngan, Pham Hong
Son, Hoang Minh
Hung, Le Van
Hoa, Tran Thi Khanh
Lan, Nguyen Thi
Lam, Truong Quang
Rozeboom, Dale
Remmenga, Marta D.
Vuolo, Matthew
Miknis, Robert
Burns, Amira
Flory, Renée
Viability of African Swine Fever Virus with the Shallow Burial with Carbon Carcass Disposal Method
title Viability of African Swine Fever Virus with the Shallow Burial with Carbon Carcass Disposal Method
title_full Viability of African Swine Fever Virus with the Shallow Burial with Carbon Carcass Disposal Method
title_fullStr Viability of African Swine Fever Virus with the Shallow Burial with Carbon Carcass Disposal Method
title_full_unstemmed Viability of African Swine Fever Virus with the Shallow Burial with Carbon Carcass Disposal Method
title_short Viability of African Swine Fever Virus with the Shallow Burial with Carbon Carcass Disposal Method
title_sort viability of african swine fever virus with the shallow burial with carbon carcass disposal method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140975/
https://www.ncbi.nlm.nih.gov/pubmed/37111514
http://dx.doi.org/10.3390/pathogens12040628
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