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Anionic Synthetic Polymers Prevent Bacteriophage Infection

[Image: see text] Bioprocessing and biotechnology exploit microorganisms (such as bacteria) for the production of chemicals, biologics, therapies, and food. A major unmet challenge is that bacteriophage (phage) contamination compromises products and necessitates shut-downs and extensive decontaminat...

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Autores principales: Marton, Huba L., Kilbride, Peter, Ahmad, Ashfaq, Sagona, Antonia P., Gibson, Matthew I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141250/
https://www.ncbi.nlm.nih.gov/pubmed/37067192
http://dx.doi.org/10.1021/jacs.3c01874
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author Marton, Huba L.
Kilbride, Peter
Ahmad, Ashfaq
Sagona, Antonia P.
Gibson, Matthew I.
author_facet Marton, Huba L.
Kilbride, Peter
Ahmad, Ashfaq
Sagona, Antonia P.
Gibson, Matthew I.
author_sort Marton, Huba L.
collection PubMed
description [Image: see text] Bioprocessing and biotechnology exploit microorganisms (such as bacteria) for the production of chemicals, biologics, therapies, and food. A major unmet challenge is that bacteriophage (phage) contamination compromises products and necessitates shut-downs and extensive decontamination using nonspecific disinfectants. Here we demonstrate that poly(acrylic acid) prevents phage-induced killing of bacterial hosts, prevents phage replication, and that induction of recombinant protein expression is not affected by the presence of the polymer. Poly(acrylic acid) was more active than poly(methacrylic acid), and poly(styrenesulfonate) had no activity showing the importance of the carboxylic acids. Initial evidence supported a virustatic, not virucidal, mechanism of action. This simple, low-cost, mass-produced additive offers a practical, scalable, and easy to implement solution to reduce phage contamination.
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spelling pubmed-101412502023-04-29 Anionic Synthetic Polymers Prevent Bacteriophage Infection Marton, Huba L. Kilbride, Peter Ahmad, Ashfaq Sagona, Antonia P. Gibson, Matthew I. J Am Chem Soc [Image: see text] Bioprocessing and biotechnology exploit microorganisms (such as bacteria) for the production of chemicals, biologics, therapies, and food. A major unmet challenge is that bacteriophage (phage) contamination compromises products and necessitates shut-downs and extensive decontamination using nonspecific disinfectants. Here we demonstrate that poly(acrylic acid) prevents phage-induced killing of bacterial hosts, prevents phage replication, and that induction of recombinant protein expression is not affected by the presence of the polymer. Poly(acrylic acid) was more active than poly(methacrylic acid), and poly(styrenesulfonate) had no activity showing the importance of the carboxylic acids. Initial evidence supported a virustatic, not virucidal, mechanism of action. This simple, low-cost, mass-produced additive offers a practical, scalable, and easy to implement solution to reduce phage contamination. American Chemical Society 2023-04-17 /pmc/articles/PMC10141250/ /pubmed/37067192 http://dx.doi.org/10.1021/jacs.3c01874 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Marton, Huba L.
Kilbride, Peter
Ahmad, Ashfaq
Sagona, Antonia P.
Gibson, Matthew I.
Anionic Synthetic Polymers Prevent Bacteriophage Infection
title Anionic Synthetic Polymers Prevent Bacteriophage Infection
title_full Anionic Synthetic Polymers Prevent Bacteriophage Infection
title_fullStr Anionic Synthetic Polymers Prevent Bacteriophage Infection
title_full_unstemmed Anionic Synthetic Polymers Prevent Bacteriophage Infection
title_short Anionic Synthetic Polymers Prevent Bacteriophage Infection
title_sort anionic synthetic polymers prevent bacteriophage infection
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141250/
https://www.ncbi.nlm.nih.gov/pubmed/37067192
http://dx.doi.org/10.1021/jacs.3c01874
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