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Structural Analysis of Bacillus subtilis Sigma Factors
Bacteria use an array of sigma factors to regulate gene expression during different stages of their life cycles. Full-length, atomic-level structures of sigma factors have been challenging to obtain experimentally as a result of their many regions of intrinsic disorder. AlphaFold has now supplied pl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141391/ https://www.ncbi.nlm.nih.gov/pubmed/37110501 http://dx.doi.org/10.3390/microorganisms11041077 |
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author | Collins, Katherine M. Evans, Nicola J. Torpey, James H. Harris, Jonathon M. Haynes, Bethany A. Camp, Amy H. Isaacson, Rivka L. |
author_facet | Collins, Katherine M. Evans, Nicola J. Torpey, James H. Harris, Jonathon M. Haynes, Bethany A. Camp, Amy H. Isaacson, Rivka L. |
author_sort | Collins, Katherine M. |
collection | PubMed |
description | Bacteria use an array of sigma factors to regulate gene expression during different stages of their life cycles. Full-length, atomic-level structures of sigma factors have been challenging to obtain experimentally as a result of their many regions of intrinsic disorder. AlphaFold has now supplied plausible full-length models for most sigma factors. Here we discuss the current understanding of the structures and functions of sigma factors in the model organism, Bacillus subtilis, and present an X-ray crystal structure of a region of B. subtilis SigE, a sigma factor that plays a critical role in the developmental process of spore formation. |
format | Online Article Text |
id | pubmed-10141391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101413912023-04-29 Structural Analysis of Bacillus subtilis Sigma Factors Collins, Katherine M. Evans, Nicola J. Torpey, James H. Harris, Jonathon M. Haynes, Bethany A. Camp, Amy H. Isaacson, Rivka L. Microorganisms Article Bacteria use an array of sigma factors to regulate gene expression during different stages of their life cycles. Full-length, atomic-level structures of sigma factors have been challenging to obtain experimentally as a result of their many regions of intrinsic disorder. AlphaFold has now supplied plausible full-length models for most sigma factors. Here we discuss the current understanding of the structures and functions of sigma factors in the model organism, Bacillus subtilis, and present an X-ray crystal structure of a region of B. subtilis SigE, a sigma factor that plays a critical role in the developmental process of spore formation. MDPI 2023-04-20 /pmc/articles/PMC10141391/ /pubmed/37110501 http://dx.doi.org/10.3390/microorganisms11041077 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Collins, Katherine M. Evans, Nicola J. Torpey, James H. Harris, Jonathon M. Haynes, Bethany A. Camp, Amy H. Isaacson, Rivka L. Structural Analysis of Bacillus subtilis Sigma Factors |
title | Structural Analysis of Bacillus subtilis Sigma Factors |
title_full | Structural Analysis of Bacillus subtilis Sigma Factors |
title_fullStr | Structural Analysis of Bacillus subtilis Sigma Factors |
title_full_unstemmed | Structural Analysis of Bacillus subtilis Sigma Factors |
title_short | Structural Analysis of Bacillus subtilis Sigma Factors |
title_sort | structural analysis of bacillus subtilis sigma factors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141391/ https://www.ncbi.nlm.nih.gov/pubmed/37110501 http://dx.doi.org/10.3390/microorganisms11041077 |
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