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Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle

Entomopathogenic fungi can be inhibited by different soil microorganisms, but the effect of a soil microbiota on fungal growth, survival, and infectivity toward insects is insufficiently understood. We investigated the level of fungistasis toward Metarhizium robertsii and Beauveria bassiana in soils...

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Autores principales: Chertkova, Ekaterina, Kabilov, Marsel R., Yaroslavtseva, Olga, Polenogova, Olga, Kosman, Elena, Sidorenko, Darya, Alikina, Tatyana, Noskov, Yury, Krivopalov, Anton, Glupov, Viktor V., Kryukov, Vadim Yu.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141481/
https://www.ncbi.nlm.nih.gov/pubmed/37110366
http://dx.doi.org/10.3390/microorganisms11040943
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author Chertkova, Ekaterina
Kabilov, Marsel R.
Yaroslavtseva, Olga
Polenogova, Olga
Kosman, Elena
Sidorenko, Darya
Alikina, Tatyana
Noskov, Yury
Krivopalov, Anton
Glupov, Viktor V.
Kryukov, Vadim Yu.
author_facet Chertkova, Ekaterina
Kabilov, Marsel R.
Yaroslavtseva, Olga
Polenogova, Olga
Kosman, Elena
Sidorenko, Darya
Alikina, Tatyana
Noskov, Yury
Krivopalov, Anton
Glupov, Viktor V.
Kryukov, Vadim Yu.
author_sort Chertkova, Ekaterina
collection PubMed
description Entomopathogenic fungi can be inhibited by different soil microorganisms, but the effect of a soil microbiota on fungal growth, survival, and infectivity toward insects is insufficiently understood. We investigated the level of fungistasis toward Metarhizium robertsii and Beauveria bassiana in soils of conventional potato fields and kitchen potato gardens. Agar diffusion methods, 16S rDNA metabarcoding, bacterial DNA quantification, and assays of Leptinotarsa decemlineata survival in soils inoculated with fungal conidia were used. Soils of kitchen gardens showed stronger fungistasis toward M. robertsii and B. bassiana and at the same time the highest density of the fungi compared to soils of conventional fields. The fungistasis level depended on the quantity of bacterial DNA and relative abundance of Bacillus, Streptomyces, and some Proteobacteria, whose abundance levels were the highest in kitchen garden soils. Cultivable isolates of bacilli exhibited antagonism to both fungi in vitro. Assays involving inoculation of nonsterile soils with B. bassiana conidia showed trends toward elevated mortality of L. decemlineata in highly fungistatic soils compared to low-fungistasis ones. Introduction of antagonistic bacilli into sterile soil did not significantly change infectivity of B. bassiana toward the insect. The results support the idea that entomopathogenic fungi can infect insects within a hypogean habitat despite high abundance and diversity of soil antagonistic bacteria.
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spelling pubmed-101414812023-04-29 Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle Chertkova, Ekaterina Kabilov, Marsel R. Yaroslavtseva, Olga Polenogova, Olga Kosman, Elena Sidorenko, Darya Alikina, Tatyana Noskov, Yury Krivopalov, Anton Glupov, Viktor V. Kryukov, Vadim Yu. Microorganisms Article Entomopathogenic fungi can be inhibited by different soil microorganisms, but the effect of a soil microbiota on fungal growth, survival, and infectivity toward insects is insufficiently understood. We investigated the level of fungistasis toward Metarhizium robertsii and Beauveria bassiana in soils of conventional potato fields and kitchen potato gardens. Agar diffusion methods, 16S rDNA metabarcoding, bacterial DNA quantification, and assays of Leptinotarsa decemlineata survival in soils inoculated with fungal conidia were used. Soils of kitchen gardens showed stronger fungistasis toward M. robertsii and B. bassiana and at the same time the highest density of the fungi compared to soils of conventional fields. The fungistasis level depended on the quantity of bacterial DNA and relative abundance of Bacillus, Streptomyces, and some Proteobacteria, whose abundance levels were the highest in kitchen garden soils. Cultivable isolates of bacilli exhibited antagonism to both fungi in vitro. Assays involving inoculation of nonsterile soils with B. bassiana conidia showed trends toward elevated mortality of L. decemlineata in highly fungistatic soils compared to low-fungistasis ones. Introduction of antagonistic bacilli into sterile soil did not significantly change infectivity of B. bassiana toward the insect. The results support the idea that entomopathogenic fungi can infect insects within a hypogean habitat despite high abundance and diversity of soil antagonistic bacteria. MDPI 2023-04-04 /pmc/articles/PMC10141481/ /pubmed/37110366 http://dx.doi.org/10.3390/microorganisms11040943 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chertkova, Ekaterina
Kabilov, Marsel R.
Yaroslavtseva, Olga
Polenogova, Olga
Kosman, Elena
Sidorenko, Darya
Alikina, Tatyana
Noskov, Yury
Krivopalov, Anton
Glupov, Viktor V.
Kryukov, Vadim Yu.
Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle
title Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle
title_full Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle
title_fullStr Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle
title_full_unstemmed Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle
title_short Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle
title_sort links between soil bacteriobiomes and fungistasis toward fungi infecting the colorado potato beetle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141481/
https://www.ncbi.nlm.nih.gov/pubmed/37110366
http://dx.doi.org/10.3390/microorganisms11040943
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