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Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Amaranthus hypochondriacus)

Two major future challenges are an increase in global earth temperature and a growing world population, which threaten agricultural productivity and nutritional food security. Underutilized crops have the potential to become future climate crops due to their high climate-resilience and nutritional q...

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Autores principales: Goel, Komal, Kundu, Pravesh, Gahlaut, Vijay, Sharma, Paras, Kumar, Ayush, Thakur, Shiwali, Verma, Vipasha, Bhargava, Bhavya, Chandora, Rahul, Zinta, Gaurav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141669/
https://www.ncbi.nlm.nih.gov/pubmed/37123843
http://dx.doi.org/10.3389/fpls.2023.1151057
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author Goel, Komal
Kundu, Pravesh
Gahlaut, Vijay
Sharma, Paras
Kumar, Ayush
Thakur, Shiwali
Verma, Vipasha
Bhargava, Bhavya
Chandora, Rahul
Zinta, Gaurav
author_facet Goel, Komal
Kundu, Pravesh
Gahlaut, Vijay
Sharma, Paras
Kumar, Ayush
Thakur, Shiwali
Verma, Vipasha
Bhargava, Bhavya
Chandora, Rahul
Zinta, Gaurav
author_sort Goel, Komal
collection PubMed
description Two major future challenges are an increase in global earth temperature and a growing world population, which threaten agricultural productivity and nutritional food security. Underutilized crops have the potential to become future climate crops due to their high climate-resilience and nutritional quality. In this context, C4 pseudocereals such as grain amaranths are very important as C4 crops are more heat tolerant than C3 crops. However, the thermal sensitivity of grain amaranths remains unexplored. Here, Amaranthus hypochondriacus was exposed to heat stress at the vegetative and reproductive stages to capture heat stress and recovery responses. Heat Shock Factors (Hsfs) form the central module to impart heat tolerance, thus we sought to identify and characterize Hsf genes. Chlorophyll content and chlorophyll fluorescence (Fv/Fm) reduced significantly during heat stress, while malondialdehyde (MDA) content increased, suggesting that heat exposure caused stress in the plants. The genome-wide analysis led to the identification of thirteen AhHsfs, which were classified into A, B and C classes. Gene expression profiling at the tissue and developmental scales resolution under heat stress revealed the transient upregulation of most of the Hsfs in the leaf and inflorescence tissues, which reverted back to control levels at the recovery time point. However, a few Hsfs somewhat sustained their upregulation during recovery phase. The study reported the identification, physical location, gene/motif structure, promoter analysis and phylogenetic relationships of Hsfs in Amaranthus hypochondriacus. Also, the genes identified may be crucial for future gene functional studies and develop thermotolerant cultivars.
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spelling pubmed-101416692023-04-29 Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Amaranthus hypochondriacus) Goel, Komal Kundu, Pravesh Gahlaut, Vijay Sharma, Paras Kumar, Ayush Thakur, Shiwali Verma, Vipasha Bhargava, Bhavya Chandora, Rahul Zinta, Gaurav Front Plant Sci Plant Science Two major future challenges are an increase in global earth temperature and a growing world population, which threaten agricultural productivity and nutritional food security. Underutilized crops have the potential to become future climate crops due to their high climate-resilience and nutritional quality. In this context, C4 pseudocereals such as grain amaranths are very important as C4 crops are more heat tolerant than C3 crops. However, the thermal sensitivity of grain amaranths remains unexplored. Here, Amaranthus hypochondriacus was exposed to heat stress at the vegetative and reproductive stages to capture heat stress and recovery responses. Heat Shock Factors (Hsfs) form the central module to impart heat tolerance, thus we sought to identify and characterize Hsf genes. Chlorophyll content and chlorophyll fluorescence (Fv/Fm) reduced significantly during heat stress, while malondialdehyde (MDA) content increased, suggesting that heat exposure caused stress in the plants. The genome-wide analysis led to the identification of thirteen AhHsfs, which were classified into A, B and C classes. Gene expression profiling at the tissue and developmental scales resolution under heat stress revealed the transient upregulation of most of the Hsfs in the leaf and inflorescence tissues, which reverted back to control levels at the recovery time point. However, a few Hsfs somewhat sustained their upregulation during recovery phase. The study reported the identification, physical location, gene/motif structure, promoter analysis and phylogenetic relationships of Hsfs in Amaranthus hypochondriacus. Also, the genes identified may be crucial for future gene functional studies and develop thermotolerant cultivars. Frontiers Media S.A. 2023-04-11 /pmc/articles/PMC10141669/ /pubmed/37123843 http://dx.doi.org/10.3389/fpls.2023.1151057 Text en Copyright © 2023 Goel, Kundu, Gahlaut, Sharma, Kumar, Thakur, Verma, Bhargava, Chandora and Zinta https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Goel, Komal
Kundu, Pravesh
Gahlaut, Vijay
Sharma, Paras
Kumar, Ayush
Thakur, Shiwali
Verma, Vipasha
Bhargava, Bhavya
Chandora, Rahul
Zinta, Gaurav
Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Amaranthus hypochondriacus)
title Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Amaranthus hypochondriacus)
title_full Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Amaranthus hypochondriacus)
title_fullStr Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Amaranthus hypochondriacus)
title_full_unstemmed Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Amaranthus hypochondriacus)
title_short Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Amaranthus hypochondriacus)
title_sort functional divergence of heat shock factors (hsfs) during heat stress and recovery at the tissue and developmental scales in c4 grain amaranth (amaranthus hypochondriacus)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141669/
https://www.ncbi.nlm.nih.gov/pubmed/37123843
http://dx.doi.org/10.3389/fpls.2023.1151057
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