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Evaluation of a Bioabsorbable Scaffold and Interlocked Nail System for Segmental Bone Defect

In the current study, we designed and manufactured a scaffold and fixation system for the reconstruction of long-bone segmental defects in a rabbit tibia model. We used biocompatible and biodegradable materials, polycaprolactone (PCL) and PCL soaked with sodium alginate (PCL-Alg) to manufacture the...

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Autores principales: Khandaker, Morshed, Lane, Reuben, Yeakley, Shannon, Alizereej, Hussein, Nikfarjam, Sadegh, Ait Moussa, Abdellah, Vaughan, Melville B., Haleem, Amgad M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141685/
https://www.ncbi.nlm.nih.gov/pubmed/37103273
http://dx.doi.org/10.3390/jfb14040183
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author Khandaker, Morshed
Lane, Reuben
Yeakley, Shannon
Alizereej, Hussein
Nikfarjam, Sadegh
Ait Moussa, Abdellah
Vaughan, Melville B.
Haleem, Amgad M.
author_facet Khandaker, Morshed
Lane, Reuben
Yeakley, Shannon
Alizereej, Hussein
Nikfarjam, Sadegh
Ait Moussa, Abdellah
Vaughan, Melville B.
Haleem, Amgad M.
author_sort Khandaker, Morshed
collection PubMed
description In the current study, we designed and manufactured a scaffold and fixation system for the reconstruction of long-bone segmental defects in a rabbit tibia model. We used biocompatible and biodegradable materials, polycaprolactone (PCL) and PCL soaked with sodium alginate (PCL-Alg) to manufacture the scaffold, interlocking nail and screws using a phase separation casing method. Degradation and mechanical tests on the PCL and PCL-Alg scaffolds indicated that both were suitable for faster degradation and early weight-bearing capacity. PCL scaffold surface porosity facilitated the infiltration of alginate hydrogel through the scaffold. Cell viability results showed that the number of cells increased on Day 7 and decreased marginally by Day 14. For accurate placement of the scaffold and fixation system, a surgical jig was designed and 3D-printed using biocompatible resin in a stereolithography (SLA) 3D printer, then cured with UV light for increased strength. Our cadaver tests using New Zealand White rabbit confirmed our novel jigs’ potential for accurate placement of the bone scaffold, intramedullary nail and the alignment of the fixation screws in future reconstructive surgeries on rabbit long-bone segmental defects. Additionally, the cadaver tests confirmed that our designed nails and screws were strong enough to carry the surgical insertion force. Therefore, our designed prototype has the potential for further clinical translational study using the rabbit tibia model.
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spelling pubmed-101416852023-04-29 Evaluation of a Bioabsorbable Scaffold and Interlocked Nail System for Segmental Bone Defect Khandaker, Morshed Lane, Reuben Yeakley, Shannon Alizereej, Hussein Nikfarjam, Sadegh Ait Moussa, Abdellah Vaughan, Melville B. Haleem, Amgad M. J Funct Biomater Article In the current study, we designed and manufactured a scaffold and fixation system for the reconstruction of long-bone segmental defects in a rabbit tibia model. We used biocompatible and biodegradable materials, polycaprolactone (PCL) and PCL soaked with sodium alginate (PCL-Alg) to manufacture the scaffold, interlocking nail and screws using a phase separation casing method. Degradation and mechanical tests on the PCL and PCL-Alg scaffolds indicated that both were suitable for faster degradation and early weight-bearing capacity. PCL scaffold surface porosity facilitated the infiltration of alginate hydrogel through the scaffold. Cell viability results showed that the number of cells increased on Day 7 and decreased marginally by Day 14. For accurate placement of the scaffold and fixation system, a surgical jig was designed and 3D-printed using biocompatible resin in a stereolithography (SLA) 3D printer, then cured with UV light for increased strength. Our cadaver tests using New Zealand White rabbit confirmed our novel jigs’ potential for accurate placement of the bone scaffold, intramedullary nail and the alignment of the fixation screws in future reconstructive surgeries on rabbit long-bone segmental defects. Additionally, the cadaver tests confirmed that our designed nails and screws were strong enough to carry the surgical insertion force. Therefore, our designed prototype has the potential for further clinical translational study using the rabbit tibia model. MDPI 2023-03-27 /pmc/articles/PMC10141685/ /pubmed/37103273 http://dx.doi.org/10.3390/jfb14040183 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khandaker, Morshed
Lane, Reuben
Yeakley, Shannon
Alizereej, Hussein
Nikfarjam, Sadegh
Ait Moussa, Abdellah
Vaughan, Melville B.
Haleem, Amgad M.
Evaluation of a Bioabsorbable Scaffold and Interlocked Nail System for Segmental Bone Defect
title Evaluation of a Bioabsorbable Scaffold and Interlocked Nail System for Segmental Bone Defect
title_full Evaluation of a Bioabsorbable Scaffold and Interlocked Nail System for Segmental Bone Defect
title_fullStr Evaluation of a Bioabsorbable Scaffold and Interlocked Nail System for Segmental Bone Defect
title_full_unstemmed Evaluation of a Bioabsorbable Scaffold and Interlocked Nail System for Segmental Bone Defect
title_short Evaluation of a Bioabsorbable Scaffold and Interlocked Nail System for Segmental Bone Defect
title_sort evaluation of a bioabsorbable scaffold and interlocked nail system for segmental bone defect
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141685/
https://www.ncbi.nlm.nih.gov/pubmed/37103273
http://dx.doi.org/10.3390/jfb14040183
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