Cargando…

Reduction of SARS-CoV-2 salivary viral load with pre-procedural mouth rinses: a randomised, controlled, clinical trial

Introduction The purpose of this study was to test the short-term efficacy of four commercial mouthwashes versus water in reducing SARS-CoV-2 viral load in the oral cavity over clinically relevant time points. Methods In total, 32 subjects that were proven SARS-CoV-2-positive via polymerase chain re...

Descripción completa

Detalles Bibliográficos
Autores principales: Farmaha, Jaspreet Kaur, James, Jeffrey N., Frazier, Kyle, Sahajpal, Nikhil Shri, Mondal, Ashis K., Bloomquist, Doan Tam, Kolhe, Ravindra, Looney, Stephen W., Bloomquist, Ryan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141803/
https://www.ncbi.nlm.nih.gov/pubmed/37117367
http://dx.doi.org/10.1038/s41415-023-5741-9
_version_ 1785033462644736000
author Farmaha, Jaspreet Kaur
James, Jeffrey N.
Frazier, Kyle
Sahajpal, Nikhil Shri
Mondal, Ashis K.
Bloomquist, Doan Tam
Kolhe, Ravindra
Looney, Stephen W.
Bloomquist, Ryan
author_facet Farmaha, Jaspreet Kaur
James, Jeffrey N.
Frazier, Kyle
Sahajpal, Nikhil Shri
Mondal, Ashis K.
Bloomquist, Doan Tam
Kolhe, Ravindra
Looney, Stephen W.
Bloomquist, Ryan
author_sort Farmaha, Jaspreet Kaur
collection PubMed
description Introduction The purpose of this study was to test the short-term efficacy of four commercial mouthwashes versus water in reducing SARS-CoV-2 viral load in the oral cavity over clinically relevant time points. Methods In total, 32 subjects that were proven SARS-CoV-2-positive via polymerase chain reaction (PCR)-based diagnostic test were recruited and randomised into five parallel arms. Cycle threshold (Ct) values were compared in saliva samples between the groups, as well as within the groups at baseline (pre-rinse), zero hours, one hour and two hours post-rinse, using SARS-CoV-2 reverse transcription-PCR analysis. Results We observed a significant increase in Ct values in saliva samples collected immediately after rinsing with all the four mouthwashes - 0.12% chlorhexidine gluconate, 1.5% hydrogen peroxide, 1% povidone iodine, or Listerine - compared to water. A sustained increase in Ct values for up to two hours was only observed in the Listerine and chlorohexidine gluconate groups. We were not able to sufficiently power this clinical trial, so the results remain notional but encouraging and supportive of findings in other emerging mouthwash studies on COVID-19, warranting additional investigations. Conclusions Our evidence suggests that in a clinical setting, prophylactic rinses with Listerine or chlorhexidine gluconate can potentially reduce SARS-CoV-2 viral load in the oral cavity for up to two hours. While limited in statistical power due to the difficulty in obtaining this data, we advocate for pre-procedural mouthwashing, like handwashing, as an economical and safe additional precaution to help mitigate the transmission of SARS-CoV-2 from a potentially infected patient to providers.
format Online
Article
Text
id pubmed-10141803
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-101418032023-05-01 Reduction of SARS-CoV-2 salivary viral load with pre-procedural mouth rinses: a randomised, controlled, clinical trial Farmaha, Jaspreet Kaur James, Jeffrey N. Frazier, Kyle Sahajpal, Nikhil Shri Mondal, Ashis K. Bloomquist, Doan Tam Kolhe, Ravindra Looney, Stephen W. Bloomquist, Ryan Br Dent J Research Introduction The purpose of this study was to test the short-term efficacy of four commercial mouthwashes versus water in reducing SARS-CoV-2 viral load in the oral cavity over clinically relevant time points. Methods In total, 32 subjects that were proven SARS-CoV-2-positive via polymerase chain reaction (PCR)-based diagnostic test were recruited and randomised into five parallel arms. Cycle threshold (Ct) values were compared in saliva samples between the groups, as well as within the groups at baseline (pre-rinse), zero hours, one hour and two hours post-rinse, using SARS-CoV-2 reverse transcription-PCR analysis. Results We observed a significant increase in Ct values in saliva samples collected immediately after rinsing with all the four mouthwashes - 0.12% chlorhexidine gluconate, 1.5% hydrogen peroxide, 1% povidone iodine, or Listerine - compared to water. A sustained increase in Ct values for up to two hours was only observed in the Listerine and chlorohexidine gluconate groups. We were not able to sufficiently power this clinical trial, so the results remain notional but encouraging and supportive of findings in other emerging mouthwash studies on COVID-19, warranting additional investigations. Conclusions Our evidence suggests that in a clinical setting, prophylactic rinses with Listerine or chlorhexidine gluconate can potentially reduce SARS-CoV-2 viral load in the oral cavity for up to two hours. While limited in statistical power due to the difficulty in obtaining this data, we advocate for pre-procedural mouthwashing, like handwashing, as an economical and safe additional precaution to help mitigate the transmission of SARS-CoV-2 from a potentially infected patient to providers. Nature Publishing Group UK 2023-04-28 2023 /pmc/articles/PMC10141803/ /pubmed/37117367 http://dx.doi.org/10.1038/s41415-023-5741-9 Text en © The Author(s), under exclusive licence to the British Dental Association 2023 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research
Farmaha, Jaspreet Kaur
James, Jeffrey N.
Frazier, Kyle
Sahajpal, Nikhil Shri
Mondal, Ashis K.
Bloomquist, Doan Tam
Kolhe, Ravindra
Looney, Stephen W.
Bloomquist, Ryan
Reduction of SARS-CoV-2 salivary viral load with pre-procedural mouth rinses: a randomised, controlled, clinical trial
title Reduction of SARS-CoV-2 salivary viral load with pre-procedural mouth rinses: a randomised, controlled, clinical trial
title_full Reduction of SARS-CoV-2 salivary viral load with pre-procedural mouth rinses: a randomised, controlled, clinical trial
title_fullStr Reduction of SARS-CoV-2 salivary viral load with pre-procedural mouth rinses: a randomised, controlled, clinical trial
title_full_unstemmed Reduction of SARS-CoV-2 salivary viral load with pre-procedural mouth rinses: a randomised, controlled, clinical trial
title_short Reduction of SARS-CoV-2 salivary viral load with pre-procedural mouth rinses: a randomised, controlled, clinical trial
title_sort reduction of sars-cov-2 salivary viral load with pre-procedural mouth rinses: a randomised, controlled, clinical trial
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141803/
https://www.ncbi.nlm.nih.gov/pubmed/37117367
http://dx.doi.org/10.1038/s41415-023-5741-9
work_keys_str_mv AT farmahajaspreetkaur reductionofsarscov2salivaryviralloadwithpreproceduralmouthrinsesarandomisedcontrolledclinicaltrial
AT jamesjeffreyn reductionofsarscov2salivaryviralloadwithpreproceduralmouthrinsesarandomisedcontrolledclinicaltrial
AT frazierkyle reductionofsarscov2salivaryviralloadwithpreproceduralmouthrinsesarandomisedcontrolledclinicaltrial
AT sahajpalnikhilshri reductionofsarscov2salivaryviralloadwithpreproceduralmouthrinsesarandomisedcontrolledclinicaltrial
AT mondalashisk reductionofsarscov2salivaryviralloadwithpreproceduralmouthrinsesarandomisedcontrolledclinicaltrial
AT bloomquistdoantam reductionofsarscov2salivaryviralloadwithpreproceduralmouthrinsesarandomisedcontrolledclinicaltrial
AT kolheravindra reductionofsarscov2salivaryviralloadwithpreproceduralmouthrinsesarandomisedcontrolledclinicaltrial
AT looneystephenw reductionofsarscov2salivaryviralloadwithpreproceduralmouthrinsesarandomisedcontrolledclinicaltrial
AT bloomquistryan reductionofsarscov2salivaryviralloadwithpreproceduralmouthrinsesarandomisedcontrolledclinicaltrial