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Unbalanced Growth, the DNA Replication Cycle and Discovery of Repair Replication

This article recounts my graduate research at Yale University (1954–1958) on unbalanced growth in Eschericia coli during thymine deprivation or following ultraviolet (UV) irradiation, with early evidence for the repair of UV-induced DNA damage. Follow-up studies in Copenhagen (1958–1960) in the labo...

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Detalles Bibliográficos
Autor principal: Hanawalt, Philip C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142232/
https://www.ncbi.nlm.nih.gov/pubmed/37109581
http://dx.doi.org/10.3390/life13041052
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author Hanawalt, Philip C.
author_facet Hanawalt, Philip C.
author_sort Hanawalt, Philip C.
collection PubMed
description This article recounts my graduate research at Yale University (1954–1958) on unbalanced growth in Eschericia coli during thymine deprivation or following ultraviolet (UV) irradiation, with early evidence for the repair of UV-induced DNA damage. Follow-up studies in Copenhagen (1958–1960) in the laboratory of Ole Maaløe led to my discovery that the DNA replication cycle can be synchronized by inhibiting protein and RNA syntheses and that an RNA synthesis step is essential for initiation of the cycle, but not for its completion. This work set the stage for my subsequent research at Stanford University, where the repair replication of damaged DNA was documented, to provide compelling evidence for an excision-repair pathway. That universal pathway validates the requirement for the redundant information in the complementary strands of duplex DNA to ensure genomic stability.
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spelling pubmed-101422322023-04-29 Unbalanced Growth, the DNA Replication Cycle and Discovery of Repair Replication Hanawalt, Philip C. Life (Basel) Perspective This article recounts my graduate research at Yale University (1954–1958) on unbalanced growth in Eschericia coli during thymine deprivation or following ultraviolet (UV) irradiation, with early evidence for the repair of UV-induced DNA damage. Follow-up studies in Copenhagen (1958–1960) in the laboratory of Ole Maaløe led to my discovery that the DNA replication cycle can be synchronized by inhibiting protein and RNA syntheses and that an RNA synthesis step is essential for initiation of the cycle, but not for its completion. This work set the stage for my subsequent research at Stanford University, where the repair replication of damaged DNA was documented, to provide compelling evidence for an excision-repair pathway. That universal pathway validates the requirement for the redundant information in the complementary strands of duplex DNA to ensure genomic stability. MDPI 2023-04-20 /pmc/articles/PMC10142232/ /pubmed/37109581 http://dx.doi.org/10.3390/life13041052 Text en © 2023 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Perspective
Hanawalt, Philip C.
Unbalanced Growth, the DNA Replication Cycle and Discovery of Repair Replication
title Unbalanced Growth, the DNA Replication Cycle and Discovery of Repair Replication
title_full Unbalanced Growth, the DNA Replication Cycle and Discovery of Repair Replication
title_fullStr Unbalanced Growth, the DNA Replication Cycle and Discovery of Repair Replication
title_full_unstemmed Unbalanced Growth, the DNA Replication Cycle and Discovery of Repair Replication
title_short Unbalanced Growth, the DNA Replication Cycle and Discovery of Repair Replication
title_sort unbalanced growth, the dna replication cycle and discovery of repair replication
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142232/
https://www.ncbi.nlm.nih.gov/pubmed/37109581
http://dx.doi.org/10.3390/life13041052
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