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Construction of high coverage whole-genome sequencing libraries from single colon crypts without DNA extraction or whole-genome amplification

OBJECTIVE: Comprehensive and reliable genome-wide variant analysis of a small number of cells has been challenging due to genome coverage bias, PCR over-cycling, and the requirement of expensive technologies. To comprehensively identify genome alterations in single colon crypts that reflect genome h...

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Detalles Bibliográficos
Autores principales:	Manojlovic, Zarko, Wlodarczyk, Jordan, Okitsu, Cindy, Jin, Yuxin, Van Den Berg, David, Lieber, Michael R., Hsieh, Chih-Lin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Research Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142246/ https://www.ncbi.nlm.nih.gov/pubmed/37106434 http://dx.doi.org/10.1186/s13104-023-06333-y

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