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Addressing the Joint Impact of Temperature and pH on Vibrio harveyi Adaptation in the Time of Climate Change

Global warming and acidification of the global ocean are two important manifestations of the ongoing climate change. To characterize their joint impact on Vibrio adaptation and fitness, we analyzed the temperature-dependent adaptation of Vibrio harveyi at different pHs (7.0, 7.5, 8.0, 8.3 and 8.5) t...

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Autores principales: Gundogdu, Kaan, Orus Iturriza, Ander, Orruño, Maite, Montánchez, Itxaso, Eguiraun, Harkaitz, Martinez, Iciar, Arana, Inés, Kaberdin, Vladimir R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142252/
https://www.ncbi.nlm.nih.gov/pubmed/37110498
http://dx.doi.org/10.3390/microorganisms11041075
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author Gundogdu, Kaan
Orus Iturriza, Ander
Orruño, Maite
Montánchez, Itxaso
Eguiraun, Harkaitz
Martinez, Iciar
Arana, Inés
Kaberdin, Vladimir R.
author_facet Gundogdu, Kaan
Orus Iturriza, Ander
Orruño, Maite
Montánchez, Itxaso
Eguiraun, Harkaitz
Martinez, Iciar
Arana, Inés
Kaberdin, Vladimir R.
author_sort Gundogdu, Kaan
collection PubMed
description Global warming and acidification of the global ocean are two important manifestations of the ongoing climate change. To characterize their joint impact on Vibrio adaptation and fitness, we analyzed the temperature-dependent adaptation of Vibrio harveyi at different pHs (7.0, 7.5, 8.0, 8.3 and 8.5) that mimic the pH of the world ocean in the past, present and future. Comparison of V. harveyi growth at 20, 25 and 30 °C show that higher temperature per se facilitates the logarithmic growth of V. harveyi in nutrient-rich environments in a pH-dependent manner. Further survival tests carried out in artificial seawater for 35 days revealed that cell culturability declined significantly upon incubation at 25 °C and 30 °C but not at 20 °C. Moreover, although acidification displayed a negative impact on cell culturability at 25 °C, it appeared to play a minor role at 30 °C, suggesting that elevated temperature, rather than pH, was the key player in the observed reduction of cell culturability. In addition, analyses of the stressed cell morphology and size distribution by epifluorescent microscopy indicates that V. harveyi likely exploits different adaptation strategies (e.g., acquisition of coccoid-like morphology) whose roles might differ depending on the temperature–pH combination.
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spelling pubmed-101422522023-04-29 Addressing the Joint Impact of Temperature and pH on Vibrio harveyi Adaptation in the Time of Climate Change Gundogdu, Kaan Orus Iturriza, Ander Orruño, Maite Montánchez, Itxaso Eguiraun, Harkaitz Martinez, Iciar Arana, Inés Kaberdin, Vladimir R. Microorganisms Article Global warming and acidification of the global ocean are two important manifestations of the ongoing climate change. To characterize their joint impact on Vibrio adaptation and fitness, we analyzed the temperature-dependent adaptation of Vibrio harveyi at different pHs (7.0, 7.5, 8.0, 8.3 and 8.5) that mimic the pH of the world ocean in the past, present and future. Comparison of V. harveyi growth at 20, 25 and 30 °C show that higher temperature per se facilitates the logarithmic growth of V. harveyi in nutrient-rich environments in a pH-dependent manner. Further survival tests carried out in artificial seawater for 35 days revealed that cell culturability declined significantly upon incubation at 25 °C and 30 °C but not at 20 °C. Moreover, although acidification displayed a negative impact on cell culturability at 25 °C, it appeared to play a minor role at 30 °C, suggesting that elevated temperature, rather than pH, was the key player in the observed reduction of cell culturability. In addition, analyses of the stressed cell morphology and size distribution by epifluorescent microscopy indicates that V. harveyi likely exploits different adaptation strategies (e.g., acquisition of coccoid-like morphology) whose roles might differ depending on the temperature–pH combination. MDPI 2023-04-20 /pmc/articles/PMC10142252/ /pubmed/37110498 http://dx.doi.org/10.3390/microorganisms11041075 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gundogdu, Kaan
Orus Iturriza, Ander
Orruño, Maite
Montánchez, Itxaso
Eguiraun, Harkaitz
Martinez, Iciar
Arana, Inés
Kaberdin, Vladimir R.
Addressing the Joint Impact of Temperature and pH on Vibrio harveyi Adaptation in the Time of Climate Change
title Addressing the Joint Impact of Temperature and pH on Vibrio harveyi Adaptation in the Time of Climate Change
title_full Addressing the Joint Impact of Temperature and pH on Vibrio harveyi Adaptation in the Time of Climate Change
title_fullStr Addressing the Joint Impact of Temperature and pH on Vibrio harveyi Adaptation in the Time of Climate Change
title_full_unstemmed Addressing the Joint Impact of Temperature and pH on Vibrio harveyi Adaptation in the Time of Climate Change
title_short Addressing the Joint Impact of Temperature and pH on Vibrio harveyi Adaptation in the Time of Climate Change
title_sort addressing the joint impact of temperature and ph on vibrio harveyi adaptation in the time of climate change
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142252/
https://www.ncbi.nlm.nih.gov/pubmed/37110498
http://dx.doi.org/10.3390/microorganisms11041075
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