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The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B

Staphylococcal enterotoxin B (SEB) is a protein produced by Staphylococcus aureus, which is toxic to humans. It is well known for its ability to stimulate the exacerbated activation of proinflammatory CD4+ T cells (Th1 profile), and in vitro studies have been conducted to understand its mechanism of...

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Autores principales: Chavez-Galan, Leslie, Ruiz, Andy, Ramón-Luing, Lucero A., Escamilla-Gutiérrez, Alejandro, Sánchez-Monciváis, Anahí, Tecuatzi-Cadena, Brenda, Medina-Quero, Karen, Córdova-Espinoza, María Guadalupe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142257/
https://www.ncbi.nlm.nih.gov/pubmed/37110712
http://dx.doi.org/10.3390/molecules28083480
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author Chavez-Galan, Leslie
Ruiz, Andy
Ramón-Luing, Lucero A.
Escamilla-Gutiérrez, Alejandro
Sánchez-Monciváis, Anahí
Tecuatzi-Cadena, Brenda
Medina-Quero, Karen
Córdova-Espinoza, María Guadalupe
author_facet Chavez-Galan, Leslie
Ruiz, Andy
Ramón-Luing, Lucero A.
Escamilla-Gutiérrez, Alejandro
Sánchez-Monciváis, Anahí
Tecuatzi-Cadena, Brenda
Medina-Quero, Karen
Córdova-Espinoza, María Guadalupe
author_sort Chavez-Galan, Leslie
collection PubMed
description Staphylococcal enterotoxin B (SEB) is a protein produced by Staphylococcus aureus, which is toxic to humans. It is well known for its ability to stimulate the exacerbated activation of proinflammatory CD4+ T cells (Th1 profile), and in vitro studies have been conducted to understand its mechanism of action and its potential use as an immune therapy. However, the efficiency of the SEB1741 aptamer in blocking SEB has not been experimentally demonstrated. Methods: Enrichment CD4+ T cells were stimulated with SEB, and as a blocker, we used the SEB1741 aptamer, which was previously synthesised by an “in silico” analysis, showing high affinity and specificity to SEB. The efficiency of the SEB1741 aptamer in blocking CD4+ T cell activation was compared with that of an anti-SEB monoclonal antibody. Flow cytometry and Bio-Plex were used to evaluate the T-cell function. Results: In vitro, SEB induced the activation of CD4+ T cells and favoured a Th1 profile; however, the SEB1741 aptamer was highly efficient in decreasing the frequency of CD4+ T cells positive to ki-67 and CD69 cells, this means that proliferation and activation of CD4+ T cells was decreased. Moreover, the production of interleukin 2 (IL-2) and interferon-gamma (IFN-γ) was affected, suggesting that the Th1 profile is not present when the SEB1441 aptamer is used. Thus, the SEB1741 function was similar to that of anti-SEB. Conclusions: The SEB1741 aptamer is a valuable tool for blocking CD4+ T cell activation and the subsequent release of proinflammatory cytokines by SEB stimulation.
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spelling pubmed-101422572023-04-29 The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B Chavez-Galan, Leslie Ruiz, Andy Ramón-Luing, Lucero A. Escamilla-Gutiérrez, Alejandro Sánchez-Monciváis, Anahí Tecuatzi-Cadena, Brenda Medina-Quero, Karen Córdova-Espinoza, María Guadalupe Molecules Article Staphylococcal enterotoxin B (SEB) is a protein produced by Staphylococcus aureus, which is toxic to humans. It is well known for its ability to stimulate the exacerbated activation of proinflammatory CD4+ T cells (Th1 profile), and in vitro studies have been conducted to understand its mechanism of action and its potential use as an immune therapy. However, the efficiency of the SEB1741 aptamer in blocking SEB has not been experimentally demonstrated. Methods: Enrichment CD4+ T cells were stimulated with SEB, and as a blocker, we used the SEB1741 aptamer, which was previously synthesised by an “in silico” analysis, showing high affinity and specificity to SEB. The efficiency of the SEB1741 aptamer in blocking CD4+ T cell activation was compared with that of an anti-SEB monoclonal antibody. Flow cytometry and Bio-Plex were used to evaluate the T-cell function. Results: In vitro, SEB induced the activation of CD4+ T cells and favoured a Th1 profile; however, the SEB1741 aptamer was highly efficient in decreasing the frequency of CD4+ T cells positive to ki-67 and CD69 cells, this means that proliferation and activation of CD4+ T cells was decreased. Moreover, the production of interleukin 2 (IL-2) and interferon-gamma (IFN-γ) was affected, suggesting that the Th1 profile is not present when the SEB1441 aptamer is used. Thus, the SEB1741 function was similar to that of anti-SEB. Conclusions: The SEB1741 aptamer is a valuable tool for blocking CD4+ T cell activation and the subsequent release of proinflammatory cytokines by SEB stimulation. MDPI 2023-04-14 /pmc/articles/PMC10142257/ /pubmed/37110712 http://dx.doi.org/10.3390/molecules28083480 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chavez-Galan, Leslie
Ruiz, Andy
Ramón-Luing, Lucero A.
Escamilla-Gutiérrez, Alejandro
Sánchez-Monciváis, Anahí
Tecuatzi-Cadena, Brenda
Medina-Quero, Karen
Córdova-Espinoza, María Guadalupe
The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B
title The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B
title_full The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B
title_fullStr The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B
title_full_unstemmed The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B
title_short The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B
title_sort seb1741 aptamer is an efficient tool for blocking cd4+ t cell activation induced by staphylococcal enterotoxin b
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142257/
https://www.ncbi.nlm.nih.gov/pubmed/37110712
http://dx.doi.org/10.3390/molecules28083480
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