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Cell-Penetrating Peptides for Use in Development of Transgenic Plants

Genetically modified plants and crops can contribute to remarkable increase in global food supply, with improved yield and resistance to plant diseases or insect pests. The development of biotechnology introducing exogenous nucleic acids in transgenic plants is important for plant health management....

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Autores principales: Liu, Betty Revon, Chen, Chi-Wei, Huang, Yue-Wern, Lee, Han-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142301/
https://www.ncbi.nlm.nih.gov/pubmed/37110602
http://dx.doi.org/10.3390/molecules28083367
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author Liu, Betty Revon
Chen, Chi-Wei
Huang, Yue-Wern
Lee, Han-Jung
author_facet Liu, Betty Revon
Chen, Chi-Wei
Huang, Yue-Wern
Lee, Han-Jung
author_sort Liu, Betty Revon
collection PubMed
description Genetically modified plants and crops can contribute to remarkable increase in global food supply, with improved yield and resistance to plant diseases or insect pests. The development of biotechnology introducing exogenous nucleic acids in transgenic plants is important for plant health management. Different genetic engineering methods for DNA delivery, such as biolistic methods, Agrobacterium tumefaciens-mediated transformation, and other physicochemical methods have been developed to improve translocation across the plasma membrane and cell wall in plants. Recently, the peptide-based gene delivery system, mediated by cell-penetrating peptides (CPPs), has been regarded as a promising non-viral tool for efficient and stable gene transfection into both animal and plant cells. CPPs are short peptides with diverse sequences and functionalities, capable of agitating plasma membrane and entering cells. Here, we highlight recent research and ideas on diverse types of CPPs, which have been applied in DNA delivery in plants. Various basic, amphipathic, cyclic, and branched CPPs were designed, and modifications of functional groups were performed to enhance DNA interaction and stabilization in transgenesis. CPPs were able to carry cargoes in either a covalent or noncovalent manner and to internalize CPP/cargo complexes into cells by either direct membrane translocation or endocytosis. Importantly, subcellular targets of CPP-mediated nucleic acid delivery were reviewed. CPPs offer transfection strategies and influence transgene expression at subcellular localizations, such as in plastids, mitochondria, and the nucleus. In summary, the technology of CPP-mediated gene delivery provides a potent and useful tool to genetically modified plants and crops of the future.
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spelling pubmed-101423012023-04-29 Cell-Penetrating Peptides for Use in Development of Transgenic Plants Liu, Betty Revon Chen, Chi-Wei Huang, Yue-Wern Lee, Han-Jung Molecules Review Genetically modified plants and crops can contribute to remarkable increase in global food supply, with improved yield and resistance to plant diseases or insect pests. The development of biotechnology introducing exogenous nucleic acids in transgenic plants is important for plant health management. Different genetic engineering methods for DNA delivery, such as biolistic methods, Agrobacterium tumefaciens-mediated transformation, and other physicochemical methods have been developed to improve translocation across the plasma membrane and cell wall in plants. Recently, the peptide-based gene delivery system, mediated by cell-penetrating peptides (CPPs), has been regarded as a promising non-viral tool for efficient and stable gene transfection into both animal and plant cells. CPPs are short peptides with diverse sequences and functionalities, capable of agitating plasma membrane and entering cells. Here, we highlight recent research and ideas on diverse types of CPPs, which have been applied in DNA delivery in plants. Various basic, amphipathic, cyclic, and branched CPPs were designed, and modifications of functional groups were performed to enhance DNA interaction and stabilization in transgenesis. CPPs were able to carry cargoes in either a covalent or noncovalent manner and to internalize CPP/cargo complexes into cells by either direct membrane translocation or endocytosis. Importantly, subcellular targets of CPP-mediated nucleic acid delivery were reviewed. CPPs offer transfection strategies and influence transgene expression at subcellular localizations, such as in plastids, mitochondria, and the nucleus. In summary, the technology of CPP-mediated gene delivery provides a potent and useful tool to genetically modified plants and crops of the future. MDPI 2023-04-11 /pmc/articles/PMC10142301/ /pubmed/37110602 http://dx.doi.org/10.3390/molecules28083367 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Liu, Betty Revon
Chen, Chi-Wei
Huang, Yue-Wern
Lee, Han-Jung
Cell-Penetrating Peptides for Use in Development of Transgenic Plants
title Cell-Penetrating Peptides for Use in Development of Transgenic Plants
title_full Cell-Penetrating Peptides for Use in Development of Transgenic Plants
title_fullStr Cell-Penetrating Peptides for Use in Development of Transgenic Plants
title_full_unstemmed Cell-Penetrating Peptides for Use in Development of Transgenic Plants
title_short Cell-Penetrating Peptides for Use in Development of Transgenic Plants
title_sort cell-penetrating peptides for use in development of transgenic plants
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142301/
https://www.ncbi.nlm.nih.gov/pubmed/37110602
http://dx.doi.org/10.3390/molecules28083367
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