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Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species

The Aspergillus genus, the etiological agent of aspergillosis, is an important food contaminant and mycotoxin producer. Plant extracts and essential oils are a source of bioactive substances with antimicrobial potential that can be used instead of synthetic food preservatives. Species from the Laura...

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Autores principales: Pinto, Leonardo de Assunção, Machado, Francisco Paiva, Esteves, Ricardo, Farias, Victor Moebus, Köptcke, Felipe Braz Nielsen, Ricci-Junior, Eduardo, Rocha, Leandro, Keller, Luiz Antonio Moura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142328/
https://www.ncbi.nlm.nih.gov/pubmed/37110671
http://dx.doi.org/10.3390/molecules28083437
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author Pinto, Leonardo de Assunção
Machado, Francisco Paiva
Esteves, Ricardo
Farias, Victor Moebus
Köptcke, Felipe Braz Nielsen
Ricci-Junior, Eduardo
Rocha, Leandro
Keller, Luiz Antonio Moura
author_facet Pinto, Leonardo de Assunção
Machado, Francisco Paiva
Esteves, Ricardo
Farias, Victor Moebus
Köptcke, Felipe Braz Nielsen
Ricci-Junior, Eduardo
Rocha, Leandro
Keller, Luiz Antonio Moura
author_sort Pinto, Leonardo de Assunção
collection PubMed
description The Aspergillus genus, the etiological agent of aspergillosis, is an important food contaminant and mycotoxin producer. Plant extracts and essential oils are a source of bioactive substances with antimicrobial potential that can be used instead of synthetic food preservatives. Species from the Lauraceae family and the Ocotea genus have been used as traditional medicinal herbs. Their essential oils can be nanoemulsified to enhance their stability and bioavailability and increase their use. Therefore, this study sought to prepare and characterize both nanoemulsion and essential oil from the Ocotea indecora’s leaves, a native and endemic species from the Mata Atlântica forest in Brazil, and evaluate the activity against Aspergillus flavus RC 2054, Aspergillus parasiticus NRRL 2999, and Aspergillus westerdjikiae NRRL 3174. The products were added to Sabouraud Dextrose Agar at concentrations of 256, 512, 1024, 2048, and 4096 µg/mL. The strains were inoculated and incubated for up to 96 h with two daily measurements. The results did not show fungicidal activity under these conditions. A fungistatic effect, however, was observed. The nanoemulsion decreased the fungistatic concentration of the essential oil more than ten times, mainly in A. westerdjikiae. There were no significant changes in aflatoxin production.
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spelling pubmed-101423282023-04-29 Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species Pinto, Leonardo de Assunção Machado, Francisco Paiva Esteves, Ricardo Farias, Victor Moebus Köptcke, Felipe Braz Nielsen Ricci-Junior, Eduardo Rocha, Leandro Keller, Luiz Antonio Moura Molecules Article The Aspergillus genus, the etiological agent of aspergillosis, is an important food contaminant and mycotoxin producer. Plant extracts and essential oils are a source of bioactive substances with antimicrobial potential that can be used instead of synthetic food preservatives. Species from the Lauraceae family and the Ocotea genus have been used as traditional medicinal herbs. Their essential oils can be nanoemulsified to enhance their stability and bioavailability and increase their use. Therefore, this study sought to prepare and characterize both nanoemulsion and essential oil from the Ocotea indecora’s leaves, a native and endemic species from the Mata Atlântica forest in Brazil, and evaluate the activity against Aspergillus flavus RC 2054, Aspergillus parasiticus NRRL 2999, and Aspergillus westerdjikiae NRRL 3174. The products were added to Sabouraud Dextrose Agar at concentrations of 256, 512, 1024, 2048, and 4096 µg/mL. The strains were inoculated and incubated for up to 96 h with two daily measurements. The results did not show fungicidal activity under these conditions. A fungistatic effect, however, was observed. The nanoemulsion decreased the fungistatic concentration of the essential oil more than ten times, mainly in A. westerdjikiae. There were no significant changes in aflatoxin production. MDPI 2023-04-13 /pmc/articles/PMC10142328/ /pubmed/37110671 http://dx.doi.org/10.3390/molecules28083437 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pinto, Leonardo de Assunção
Machado, Francisco Paiva
Esteves, Ricardo
Farias, Victor Moebus
Köptcke, Felipe Braz Nielsen
Ricci-Junior, Eduardo
Rocha, Leandro
Keller, Luiz Antonio Moura
Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species
title Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species
title_full Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species
title_fullStr Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species
title_full_unstemmed Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species
title_short Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species
title_sort characterization and inhibitory effects of essential oil and nanoemulsion from ocotea indecora (shott) mez in aspergillus species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142328/
https://www.ncbi.nlm.nih.gov/pubmed/37110671
http://dx.doi.org/10.3390/molecules28083437
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