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Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos
Difenoconazole is a type of triazole fungicide that is widely used in the treatment of plant diseases. Triazole fungicides have been shown in several studies to impair the development of the nervous system in zebrafish embryos. There is still little known about difenoconazole-induced neurotoxicity i...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142703/ https://www.ncbi.nlm.nih.gov/pubmed/37112580 http://dx.doi.org/10.3390/toxics11040353 |
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author | Yang, Qing Deng, Ping Xing, Dan Liu, Haoling Shi, Fang Hu, Lian Zou, Xi Nie, Hongyan Zuo, Junli Zhuang, Zimeng Pan, Meiqi Chen, Juan Li, Guangyu |
author_facet | Yang, Qing Deng, Ping Xing, Dan Liu, Haoling Shi, Fang Hu, Lian Zou, Xi Nie, Hongyan Zuo, Junli Zhuang, Zimeng Pan, Meiqi Chen, Juan Li, Guangyu |
author_sort | Yang, Qing |
collection | PubMed |
description | Difenoconazole is a type of triazole fungicide that is widely used in the treatment of plant diseases. Triazole fungicides have been shown in several studies to impair the development of the nervous system in zebrafish embryos. There is still little known about difenoconazole-induced neurotoxicity in fish. In this study, zebrafish embryos were exposed to 0.25, 0.5, and 1 mg/L of difenoconazole solution until 120 h post-fertilization (hpf). The difenoconazole-exposed groups showed concentration-dependent inhibitory tendencies in heart rate and body length. Malformation rate and spontaneous movement of zebrafish embryos increased, and the locomotor activity decreased in the highest exposure group. The content of dopamine and acetylcholine was reduced significantly in difenoconazole treatment groups. The activity of acetylcholinesterase (AChE) was also increased after treatment with difenoconazole. Furthermore, the expression of genes involved in neurodevelopment was remarkably altered, which corresponded with the alterations of neurotransmitter content and AChE activity. These results indicated that difenoconazole might affect the development of the nervous system through influencing neurotransmitter levels, enzyme activity, and the expression of neural-related genes, ultimately leading to abnormal locomotor activity in the early stages of zebrafish. |
format | Online Article Text |
id | pubmed-10142703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101427032023-04-29 Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos Yang, Qing Deng, Ping Xing, Dan Liu, Haoling Shi, Fang Hu, Lian Zou, Xi Nie, Hongyan Zuo, Junli Zhuang, Zimeng Pan, Meiqi Chen, Juan Li, Guangyu Toxics Article Difenoconazole is a type of triazole fungicide that is widely used in the treatment of plant diseases. Triazole fungicides have been shown in several studies to impair the development of the nervous system in zebrafish embryos. There is still little known about difenoconazole-induced neurotoxicity in fish. In this study, zebrafish embryos were exposed to 0.25, 0.5, and 1 mg/L of difenoconazole solution until 120 h post-fertilization (hpf). The difenoconazole-exposed groups showed concentration-dependent inhibitory tendencies in heart rate and body length. Malformation rate and spontaneous movement of zebrafish embryos increased, and the locomotor activity decreased in the highest exposure group. The content of dopamine and acetylcholine was reduced significantly in difenoconazole treatment groups. The activity of acetylcholinesterase (AChE) was also increased after treatment with difenoconazole. Furthermore, the expression of genes involved in neurodevelopment was remarkably altered, which corresponded with the alterations of neurotransmitter content and AChE activity. These results indicated that difenoconazole might affect the development of the nervous system through influencing neurotransmitter levels, enzyme activity, and the expression of neural-related genes, ultimately leading to abnormal locomotor activity in the early stages of zebrafish. MDPI 2023-04-08 /pmc/articles/PMC10142703/ /pubmed/37112580 http://dx.doi.org/10.3390/toxics11040353 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Qing Deng, Ping Xing, Dan Liu, Haoling Shi, Fang Hu, Lian Zou, Xi Nie, Hongyan Zuo, Junli Zhuang, Zimeng Pan, Meiqi Chen, Juan Li, Guangyu Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos |
title | Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos |
title_full | Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos |
title_fullStr | Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos |
title_full_unstemmed | Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos |
title_short | Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos |
title_sort | developmental neurotoxicity of difenoconazole in zebrafish embryos |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142703/ https://www.ncbi.nlm.nih.gov/pubmed/37112580 http://dx.doi.org/10.3390/toxics11040353 |
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