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Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Neolamarckia cadamba

The WRKY transcription factor family plays important regulatory roles in multiple biological processes in higher plants. They have been identified and functionally characterized in a number of plant species, but very little is known in Neolamarckia cadamba, a ‘miracle tree’ for its fast growth and p...

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Autores principales: Xu, Zuowei, Liu, Yutong, Fang, Huiting, Wen, Yanqiong, Wang, Ying, Zhang, Jianxia, Peng, Changcao, Long, Jianmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142840/
https://www.ncbi.nlm.nih.gov/pubmed/37108700
http://dx.doi.org/10.3390/ijms24087537
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author Xu, Zuowei
Liu, Yutong
Fang, Huiting
Wen, Yanqiong
Wang, Ying
Zhang, Jianxia
Peng, Changcao
Long, Jianmei
author_facet Xu, Zuowei
Liu, Yutong
Fang, Huiting
Wen, Yanqiong
Wang, Ying
Zhang, Jianxia
Peng, Changcao
Long, Jianmei
author_sort Xu, Zuowei
collection PubMed
description The WRKY transcription factor family plays important regulatory roles in multiple biological processes in higher plants. They have been identified and functionally characterized in a number of plant species, but very little is known in Neolamarckia cadamba, a ‘miracle tree’ for its fast growth and potential medicinal resource in Southeast Asia. In this study, a total of 85 WRKY genes were identified in the genome of N. cadamba. They were divided into three groups according to their phylogenetic features, with the support of the characteristics of gene structures and conserved motifs of protein. The NcWRKY genes were unevenly distributed on 22 chromosomes, and there were two pairs of segmentally duplicated events. In addition, a number of putative cis-elements were identified in the promoter regions, of which hormone- and stress-related elements were shared in many NcWRKYs. The transcript levels of NcWRKY were analyzed using the RNA-seq data, revealing distinct expression patterns in various tissues and at different stages of vascular development. Furthermore, 16 and 12 NcWRKY genes were confirmed to respond to various hormone treatments and two different abiotic stress treatments, respectively. Moreover, the content of cadambine, the active metabolite used for the various pharmacological activities found in N. cadamba, significantly increased after Methyl jasmonate treatment. In addition, expression of NcWRKY64/74 was obviously upregulated, suggesting that they may have a potential function of regulating the biosynthesis of cadambine in response to MeJA. Taken together, this study provides clues into the regulatory roles of the WRKY gene family in N. cadamba.
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spelling pubmed-101428402023-04-29 Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Neolamarckia cadamba Xu, Zuowei Liu, Yutong Fang, Huiting Wen, Yanqiong Wang, Ying Zhang, Jianxia Peng, Changcao Long, Jianmei Int J Mol Sci Article The WRKY transcription factor family plays important regulatory roles in multiple biological processes in higher plants. They have been identified and functionally characterized in a number of plant species, but very little is known in Neolamarckia cadamba, a ‘miracle tree’ for its fast growth and potential medicinal resource in Southeast Asia. In this study, a total of 85 WRKY genes were identified in the genome of N. cadamba. They were divided into three groups according to their phylogenetic features, with the support of the characteristics of gene structures and conserved motifs of protein. The NcWRKY genes were unevenly distributed on 22 chromosomes, and there were two pairs of segmentally duplicated events. In addition, a number of putative cis-elements were identified in the promoter regions, of which hormone- and stress-related elements were shared in many NcWRKYs. The transcript levels of NcWRKY were analyzed using the RNA-seq data, revealing distinct expression patterns in various tissues and at different stages of vascular development. Furthermore, 16 and 12 NcWRKY genes were confirmed to respond to various hormone treatments and two different abiotic stress treatments, respectively. Moreover, the content of cadambine, the active metabolite used for the various pharmacological activities found in N. cadamba, significantly increased after Methyl jasmonate treatment. In addition, expression of NcWRKY64/74 was obviously upregulated, suggesting that they may have a potential function of regulating the biosynthesis of cadambine in response to MeJA. Taken together, this study provides clues into the regulatory roles of the WRKY gene family in N. cadamba. MDPI 2023-04-19 /pmc/articles/PMC10142840/ /pubmed/37108700 http://dx.doi.org/10.3390/ijms24087537 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Zuowei
Liu, Yutong
Fang, Huiting
Wen, Yanqiong
Wang, Ying
Zhang, Jianxia
Peng, Changcao
Long, Jianmei
Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Neolamarckia cadamba
title Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Neolamarckia cadamba
title_full Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Neolamarckia cadamba
title_fullStr Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Neolamarckia cadamba
title_full_unstemmed Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Neolamarckia cadamba
title_short Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Neolamarckia cadamba
title_sort genome-wide identification and expression analysis of wrky gene family in neolamarckia cadamba
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142840/
https://www.ncbi.nlm.nih.gov/pubmed/37108700
http://dx.doi.org/10.3390/ijms24087537
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