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Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K
A novel porous polyoxometalate (POM)-based composite (Co(4)PW–PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co(4)PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was con...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142915/ https://www.ncbi.nlm.nih.gov/pubmed/37110541 http://dx.doi.org/10.3390/molecules28083307 |
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author | Yang, Jiaxuan Chu, Ning Chen, Xuwei |
author_facet | Yang, Jiaxuan Chu, Ning Chen, Xuwei |
author_sort | Yang, Jiaxuan |
collection | PubMed |
description | A novel porous polyoxometalate (POM)-based composite (Co(4)PW–PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co(4)PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was confirmed by EDS, SEM, FT-IR, TGA, and so on. The strong covalent coordination and hydrogen-bonding interaction between the highly active Co(2+) of the Co(4)PW and the aspartic acid residues of proteinase K endowed the obtained Co(4)PW–PDDVAC composite with excellent proteinase K adsorption properties. Thermodynamic investigations indicate that the adsorption behavior of proteinase K was consistent with the linear Langmuir isothermal model, giving an adsorption capacity as high as 1428 mg g(−1). The Co(4)PW–PDDVAC composite was applied in the selective isolation of highly active proteinase K from Tritirachium album Limber crude enzyme fluid. |
format | Online Article Text |
id | pubmed-10142915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101429152023-04-29 Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K Yang, Jiaxuan Chu, Ning Chen, Xuwei Molecules Article A novel porous polyoxometalate (POM)-based composite (Co(4)PW–PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co(4)PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was confirmed by EDS, SEM, FT-IR, TGA, and so on. The strong covalent coordination and hydrogen-bonding interaction between the highly active Co(2+) of the Co(4)PW and the aspartic acid residues of proteinase K endowed the obtained Co(4)PW–PDDVAC composite with excellent proteinase K adsorption properties. Thermodynamic investigations indicate that the adsorption behavior of proteinase K was consistent with the linear Langmuir isothermal model, giving an adsorption capacity as high as 1428 mg g(−1). The Co(4)PW–PDDVAC composite was applied in the selective isolation of highly active proteinase K from Tritirachium album Limber crude enzyme fluid. MDPI 2023-04-07 /pmc/articles/PMC10142915/ /pubmed/37110541 http://dx.doi.org/10.3390/molecules28083307 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Jiaxuan Chu, Ning Chen, Xuwei Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
title | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
title_full | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
title_fullStr | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
title_full_unstemmed | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
title_short | Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K |
title_sort | preparation of polyoxometalate-based composite by solidification of highly active cobalt-containing polytungstate on polymeric ionic liquid for the efficient isolation of proteinase k |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142915/ https://www.ncbi.nlm.nih.gov/pubmed/37110541 http://dx.doi.org/10.3390/molecules28083307 |
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