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Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K

A novel porous polyoxometalate (POM)-based composite (Co(4)PW–PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co(4)PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was con...

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Autores principales: Yang, Jiaxuan, Chu, Ning, Chen, Xuwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142915/
https://www.ncbi.nlm.nih.gov/pubmed/37110541
http://dx.doi.org/10.3390/molecules28083307
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author Yang, Jiaxuan
Chu, Ning
Chen, Xuwei
author_facet Yang, Jiaxuan
Chu, Ning
Chen, Xuwei
author_sort Yang, Jiaxuan
collection PubMed
description A novel porous polyoxometalate (POM)-based composite (Co(4)PW–PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co(4)PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was confirmed by EDS, SEM, FT-IR, TGA, and so on. The strong covalent coordination and hydrogen-bonding interaction between the highly active Co(2+) of the Co(4)PW and the aspartic acid residues of proteinase K endowed the obtained Co(4)PW–PDDVAC composite with excellent proteinase K adsorption properties. Thermodynamic investigations indicate that the adsorption behavior of proteinase K was consistent with the linear Langmuir isothermal model, giving an adsorption capacity as high as 1428 mg g(−1). The Co(4)PW–PDDVAC composite was applied in the selective isolation of highly active proteinase K from Tritirachium album Limber crude enzyme fluid.
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spelling pubmed-101429152023-04-29 Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K Yang, Jiaxuan Chu, Ning Chen, Xuwei Molecules Article A novel porous polyoxometalate (POM)-based composite (Co(4)PW–PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co(4)PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was confirmed by EDS, SEM, FT-IR, TGA, and so on. The strong covalent coordination and hydrogen-bonding interaction between the highly active Co(2+) of the Co(4)PW and the aspartic acid residues of proteinase K endowed the obtained Co(4)PW–PDDVAC composite with excellent proteinase K adsorption properties. Thermodynamic investigations indicate that the adsorption behavior of proteinase K was consistent with the linear Langmuir isothermal model, giving an adsorption capacity as high as 1428 mg g(−1). The Co(4)PW–PDDVAC composite was applied in the selective isolation of highly active proteinase K from Tritirachium album Limber crude enzyme fluid. MDPI 2023-04-07 /pmc/articles/PMC10142915/ /pubmed/37110541 http://dx.doi.org/10.3390/molecules28083307 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Jiaxuan
Chu, Ning
Chen, Xuwei
Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K
title Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K
title_full Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K
title_fullStr Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K
title_full_unstemmed Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K
title_short Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K
title_sort preparation of polyoxometalate-based composite by solidification of highly active cobalt-containing polytungstate on polymeric ionic liquid for the efficient isolation of proteinase k
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142915/
https://www.ncbi.nlm.nih.gov/pubmed/37110541
http://dx.doi.org/10.3390/molecules28083307
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