First Isolation and Genome Sequence Analysis of West Nile Virus in Mosquitoes in Brazil

West Nile virus is a flavivirus transmitted by mosquitoes, mainly of the genus Culex. In Brazil, serological studies have already indicated the circulation of the virus since 2003, with the first human case detected in 2014. The objective of the present paper is to report the first isolation of WNV...

Descripción completa

Detalles Bibliográficos
Autores principales: Neto, Joaquim Pinto Nunes, Reis, Lúcia Aline Moura, Freitas, Maria Nazaré Oliveira, do Nascimento, Bruna Laís Sena, das Chagas, Liliane Leal, da Costa, Hernan Hermes Monteiro, Rodrigues, Jéssica Cecília Pinheiro, Braga, Camila Margalho, da Silva, Eliana Vieira Pinto, Silva, Sandro Patroca, Martins, Lívia Caricio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143329/
https://www.ncbi.nlm.nih.gov/pubmed/37104362
http://dx.doi.org/10.3390/tropicalmed8040237
Descripción
Sumario:West Nile virus is a flavivirus transmitted by mosquitoes, mainly of the genus Culex. In Brazil, serological studies have already indicated the circulation of the virus since 2003, with the first human case detected in 2014. The objective of the present paper is to report the first isolation of WNV in a Culex (Melanoconion) mosquito. Arthropods were collected by protected human attraction and CDC light bait, and taxonomically identified and analyzed by viral isolation, complement fixation and genomic sequencing tests. WNV was isolated from samples of Culex (Melanoconion) mosquitoes, and the sequencing analysis demonstrated that the isolated strain belonged to lineage 1a. The finding of the present study presents the first evidence of the isolation and genome sequencing of WNV in arthropods in Brazil.

Ejemplares similares