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Development of a Multicomponent Microbiological Soil Inoculant and Its Performance in Sweet Potato Cultivation
The cultivation and consumption of sweet potato (Ipomoea batatas) are increasing globally. As the usage of chemical fertilizers and pest control agents during its cultivation may lead to soil, water and air pollution, there is an emerging need for environment-friendly, biological solutions enabling...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143537/ https://www.ncbi.nlm.nih.gov/pubmed/37110337 http://dx.doi.org/10.3390/microorganisms11040914 |
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author | Nagy, Viktor Dávid Zhumakayev, Anuar Vörös, Mónika Bordé, Ádám Szarvas, Adrienn Szűcs, Attila Kocsubé, Sándor Jakab, Péter Monostori, Tamás Škrbić, Biljana D. Mohai, Edina Hatvani, Lóránt Vágvölgyi, Csaba Kredics, László |
author_facet | Nagy, Viktor Dávid Zhumakayev, Anuar Vörös, Mónika Bordé, Ádám Szarvas, Adrienn Szűcs, Attila Kocsubé, Sándor Jakab, Péter Monostori, Tamás Škrbić, Biljana D. Mohai, Edina Hatvani, Lóránt Vágvölgyi, Csaba Kredics, László |
author_sort | Nagy, Viktor Dávid |
collection | PubMed |
description | The cultivation and consumption of sweet potato (Ipomoea batatas) are increasing globally. As the usage of chemical fertilizers and pest control agents during its cultivation may lead to soil, water and air pollution, there is an emerging need for environment-friendly, biological solutions enabling increased amounts of healthy crop and efficient disease management. Microbiological agents for agricultural purposes gained increasing importance in the past few decades. Our goal was to develop an agricultural soil inoculant from multiple microorganisms and test its application potential in sweet potato cultivation. Two Trichoderma strains were selected: Trichoderma ghanense strain SZMC 25217 based on its extracellular enzyme activities for the biodegradation of plant residues, and Trichoderma afroharzianum strain SZMC 25231 for biocontrol purposes against fungal plant pathogens. The Bacillus velezensis strain SZMC 24986 proved to be the best growth inhibitor of most of the nine tested strains of fungal species known as plant pathogens, therefore it was also selected for biocontrol purposes against fungal plant pathogens. Arthrobacter globiformis strain SZMC 25081, showing the fastest growth on nitrogen-free medium, was selected as a component with possible nitrogen-fixing potential. A Pseudomonas resinovorans strain, SZMC 25872, was selected for its ability to produce indole-3-acetic acid, which is among the important traits of potential plant growth-promoting rhizobacteria (PGPR). A series of experiments were performed to test the selected strains for their tolerance to abiotic stress factors such as pH, temperature, water activity and fungicides, influencing the survivability in agricultural environments. The selected strains were used to treat sweet potato in two separate field experiments. Yield increase was observed for the plants treated with the selected microbial consortium (synthetic community) in comparison with the control group in both cases. Our results suggest that the developed microbial inoculant has the potential to be used in sweet potato plantations. To the best of our knowledge, this is the first report about the successful application of a fungal-bacterial consortium in sweet potato cultivation. |
format | Online Article Text |
id | pubmed-10143537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101435372023-04-29 Development of a Multicomponent Microbiological Soil Inoculant and Its Performance in Sweet Potato Cultivation Nagy, Viktor Dávid Zhumakayev, Anuar Vörös, Mónika Bordé, Ádám Szarvas, Adrienn Szűcs, Attila Kocsubé, Sándor Jakab, Péter Monostori, Tamás Škrbić, Biljana D. Mohai, Edina Hatvani, Lóránt Vágvölgyi, Csaba Kredics, László Microorganisms Article The cultivation and consumption of sweet potato (Ipomoea batatas) are increasing globally. As the usage of chemical fertilizers and pest control agents during its cultivation may lead to soil, water and air pollution, there is an emerging need for environment-friendly, biological solutions enabling increased amounts of healthy crop and efficient disease management. Microbiological agents for agricultural purposes gained increasing importance in the past few decades. Our goal was to develop an agricultural soil inoculant from multiple microorganisms and test its application potential in sweet potato cultivation. Two Trichoderma strains were selected: Trichoderma ghanense strain SZMC 25217 based on its extracellular enzyme activities for the biodegradation of plant residues, and Trichoderma afroharzianum strain SZMC 25231 for biocontrol purposes against fungal plant pathogens. The Bacillus velezensis strain SZMC 24986 proved to be the best growth inhibitor of most of the nine tested strains of fungal species known as plant pathogens, therefore it was also selected for biocontrol purposes against fungal plant pathogens. Arthrobacter globiformis strain SZMC 25081, showing the fastest growth on nitrogen-free medium, was selected as a component with possible nitrogen-fixing potential. A Pseudomonas resinovorans strain, SZMC 25872, was selected for its ability to produce indole-3-acetic acid, which is among the important traits of potential plant growth-promoting rhizobacteria (PGPR). A series of experiments were performed to test the selected strains for their tolerance to abiotic stress factors such as pH, temperature, water activity and fungicides, influencing the survivability in agricultural environments. The selected strains were used to treat sweet potato in two separate field experiments. Yield increase was observed for the plants treated with the selected microbial consortium (synthetic community) in comparison with the control group in both cases. Our results suggest that the developed microbial inoculant has the potential to be used in sweet potato plantations. To the best of our knowledge, this is the first report about the successful application of a fungal-bacterial consortium in sweet potato cultivation. MDPI 2023-03-31 /pmc/articles/PMC10143537/ /pubmed/37110337 http://dx.doi.org/10.3390/microorganisms11040914 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nagy, Viktor Dávid Zhumakayev, Anuar Vörös, Mónika Bordé, Ádám Szarvas, Adrienn Szűcs, Attila Kocsubé, Sándor Jakab, Péter Monostori, Tamás Škrbić, Biljana D. Mohai, Edina Hatvani, Lóránt Vágvölgyi, Csaba Kredics, László Development of a Multicomponent Microbiological Soil Inoculant and Its Performance in Sweet Potato Cultivation |
title | Development of a Multicomponent Microbiological Soil Inoculant and Its Performance in Sweet Potato Cultivation |
title_full | Development of a Multicomponent Microbiological Soil Inoculant and Its Performance in Sweet Potato Cultivation |
title_fullStr | Development of a Multicomponent Microbiological Soil Inoculant and Its Performance in Sweet Potato Cultivation |
title_full_unstemmed | Development of a Multicomponent Microbiological Soil Inoculant and Its Performance in Sweet Potato Cultivation |
title_short | Development of a Multicomponent Microbiological Soil Inoculant and Its Performance in Sweet Potato Cultivation |
title_sort | development of a multicomponent microbiological soil inoculant and its performance in sweet potato cultivation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143537/ https://www.ncbi.nlm.nih.gov/pubmed/37110337 http://dx.doi.org/10.3390/microorganisms11040914 |
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