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Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites: Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH

The aim of this study was to investigate methylone and its metabolites concentration in oral fluid following controlled increasing doses, focusing on the effect of oral fluid pH. Samples were obtained from a clinical trial where twelve healthy volunteers participated after ingestion of 50, 100, 150...

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Autores principales: Sprega, Giorgia, Di Giorgi, Alessandro, Poyatos, Lourdes, Papaseit, Esther, Pérez-Mañá, Clara, Tini, Anastasio, Pichini, Simona, Busardò, Francesco Paolo, Lo Faro, Alfredo Fabrizio, Farré, Magí
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143603/
https://www.ncbi.nlm.nih.gov/pubmed/37110127
http://dx.doi.org/10.3390/metabo13040468
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author Sprega, Giorgia
Di Giorgi, Alessandro
Poyatos, Lourdes
Papaseit, Esther
Pérez-Mañá, Clara
Tini, Anastasio
Pichini, Simona
Busardò, Francesco Paolo
Lo Faro, Alfredo Fabrizio
Farré, Magí
author_facet Sprega, Giorgia
Di Giorgi, Alessandro
Poyatos, Lourdes
Papaseit, Esther
Pérez-Mañá, Clara
Tini, Anastasio
Pichini, Simona
Busardò, Francesco Paolo
Lo Faro, Alfredo Fabrizio
Farré, Magí
author_sort Sprega, Giorgia
collection PubMed
description The aim of this study was to investigate methylone and its metabolites concentration in oral fluid following controlled increasing doses, focusing on the effect of oral fluid pH. Samples were obtained from a clinical trial where twelve healthy volunteers participated after ingestion of 50, 100, 150 and 200 mg of methylone. Concentration of methylone and its metabolites 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone in oral fluid were measured using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Pharmacokinetic parameters were estimated, and the oral fluid-to-plasma ratio (OF/P) at each time interval was calculated and correlated with the oral fluid pH using data from our previous study in plasma. Methylone was detected at all time intervals after each dose; MDC and HMMC were not detectable after the lowest dose. Oral fluid concentrations of methylone ranged between 88.3–503.8, 85.5–5002.3, 182.8–13,201.8 and 214.6–22,684.6 ng/mL following 50, 100, 150 and 200 mg doses, respectively, peaked between 1.5 and 2.0 h, and were followed by a progressive decrease. Oral fluid pH was demonstrated to be affected by methylone administration. Oral fluid is a valid alternative to plasma for methylone determination for clinical and toxicological studies, allowing for a simple, easy and non-invasive sample collection.
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spelling pubmed-101436032023-04-29 Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites: Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH Sprega, Giorgia Di Giorgi, Alessandro Poyatos, Lourdes Papaseit, Esther Pérez-Mañá, Clara Tini, Anastasio Pichini, Simona Busardò, Francesco Paolo Lo Faro, Alfredo Fabrizio Farré, Magí Metabolites Article The aim of this study was to investigate methylone and its metabolites concentration in oral fluid following controlled increasing doses, focusing on the effect of oral fluid pH. Samples were obtained from a clinical trial where twelve healthy volunteers participated after ingestion of 50, 100, 150 and 200 mg of methylone. Concentration of methylone and its metabolites 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone in oral fluid were measured using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Pharmacokinetic parameters were estimated, and the oral fluid-to-plasma ratio (OF/P) at each time interval was calculated and correlated with the oral fluid pH using data from our previous study in plasma. Methylone was detected at all time intervals after each dose; MDC and HMMC were not detectable after the lowest dose. Oral fluid concentrations of methylone ranged between 88.3–503.8, 85.5–5002.3, 182.8–13,201.8 and 214.6–22,684.6 ng/mL following 50, 100, 150 and 200 mg doses, respectively, peaked between 1.5 and 2.0 h, and were followed by a progressive decrease. Oral fluid pH was demonstrated to be affected by methylone administration. Oral fluid is a valid alternative to plasma for methylone determination for clinical and toxicological studies, allowing for a simple, easy and non-invasive sample collection. MDPI 2023-03-24 /pmc/articles/PMC10143603/ /pubmed/37110127 http://dx.doi.org/10.3390/metabo13040468 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sprega, Giorgia
Di Giorgi, Alessandro
Poyatos, Lourdes
Papaseit, Esther
Pérez-Mañá, Clara
Tini, Anastasio
Pichini, Simona
Busardò, Francesco Paolo
Lo Faro, Alfredo Fabrizio
Farré, Magí
Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites: Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH
title Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites: Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH
title_full Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites: Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH
title_fullStr Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites: Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH
title_full_unstemmed Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites: Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH
title_short Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites: Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH
title_sort usefulness of oral fluid for measurement of methylone and its metabolites: correlation with plasma drug concentrations and the effect of oral fluid ph
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143603/
https://www.ncbi.nlm.nih.gov/pubmed/37110127
http://dx.doi.org/10.3390/metabo13040468
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