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Laboratory Evaluation and Field Testing of Dengue NS1 and IgM/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal

In Senegal, the burden of dengue is increasing and expanding. As case management and traditional diagnostic techniques can be difficult to implement, rapid diagnostic tests (RDTs) deployed at point of care are ideal for investigating active outbreaks. The aim of this study was to evaluate the diagno...

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Autores principales: Ndiaye, Oumar, Woolston, Kevin, Gaye, Aboubacry, Loucoubar, Cheikh, Cocozza, Michael, Fall, Cheikh, Dia, Fatou, Adams, Emily R., Samb, Marième, Camara, Diogop, Sadio, Bacary Djilocalisse, Diagne, Cheikh T., Weidmann, Manfred, Faye, Oumar, Fitchett, Joseph R. A., Sall, Amadou Alpha, Diagne, Cheikh Tidiane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143717/
https://www.ncbi.nlm.nih.gov/pubmed/37112887
http://dx.doi.org/10.3390/v15040904
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author Ndiaye, Oumar
Woolston, Kevin
Gaye, Aboubacry
Loucoubar, Cheikh
Cocozza, Michael
Fall, Cheikh
Dia, Fatou
Adams, Emily R.
Samb, Marième
Camara, Diogop
Sadio, Bacary Djilocalisse
Diagne, Cheikh T.
Weidmann, Manfred
Faye, Oumar
Fitchett, Joseph R. A.
Sall, Amadou Alpha
Diagne, Cheikh Tidiane
author_facet Ndiaye, Oumar
Woolston, Kevin
Gaye, Aboubacry
Loucoubar, Cheikh
Cocozza, Michael
Fall, Cheikh
Dia, Fatou
Adams, Emily R.
Samb, Marième
Camara, Diogop
Sadio, Bacary Djilocalisse
Diagne, Cheikh T.
Weidmann, Manfred
Faye, Oumar
Fitchett, Joseph R. A.
Sall, Amadou Alpha
Diagne, Cheikh Tidiane
author_sort Ndiaye, Oumar
collection PubMed
description In Senegal, the burden of dengue is increasing and expanding. As case management and traditional diagnostic techniques can be difficult to implement, rapid diagnostic tests (RDTs) deployed at point of care are ideal for investigating active outbreaks. The aim of this study was to evaluate the diagnostic performance of the Dengue NS1 and Dengue IgM/IgG RDTs on the serum/plasma samples in a laboratory setting and in the field. During laboratory evaluation, performance of the NS1 RDT was assessed using NS1 ELISA as the gold standard. Sensitivity and specificity were 88% [75–95%] and 100% [97–100%], respectively. Performance of the IgM/IG RDT was assessed using the IgM Antibody Capture (MAC) ELISA, indirect IgG, and PRNT as gold standards. The IgM and IgG test lines respectively displayed sensitivities of 94% [83–99%] and 70% [59–79%] and specificities of 91% [84–95%] and 91% [79–98%]. In the field, the Dengue NS1 RDT sensitivity and specificity was 82% [60–95%] and 75% [53–90%], respectively. The IgM and IgG test lines displayed sensitivities of 86% [42–100%] and 78% [64–88%], specificities of 85% [76–92%] and 55% [36–73%], respectively. These results demonstrate that RDTs are ideal for use in a context of high prevalence or outbreak setting and can be implemented in the absence of a confirmatory test for acute and convalescent patients.
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spelling pubmed-101437172023-04-29 Laboratory Evaluation and Field Testing of Dengue NS1 and IgM/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal Ndiaye, Oumar Woolston, Kevin Gaye, Aboubacry Loucoubar, Cheikh Cocozza, Michael Fall, Cheikh Dia, Fatou Adams, Emily R. Samb, Marième Camara, Diogop Sadio, Bacary Djilocalisse Diagne, Cheikh T. Weidmann, Manfred Faye, Oumar Fitchett, Joseph R. A. Sall, Amadou Alpha Diagne, Cheikh Tidiane Viruses Article In Senegal, the burden of dengue is increasing and expanding. As case management and traditional diagnostic techniques can be difficult to implement, rapid diagnostic tests (RDTs) deployed at point of care are ideal for investigating active outbreaks. The aim of this study was to evaluate the diagnostic performance of the Dengue NS1 and Dengue IgM/IgG RDTs on the serum/plasma samples in a laboratory setting and in the field. During laboratory evaluation, performance of the NS1 RDT was assessed using NS1 ELISA as the gold standard. Sensitivity and specificity were 88% [75–95%] and 100% [97–100%], respectively. Performance of the IgM/IG RDT was assessed using the IgM Antibody Capture (MAC) ELISA, indirect IgG, and PRNT as gold standards. The IgM and IgG test lines respectively displayed sensitivities of 94% [83–99%] and 70% [59–79%] and specificities of 91% [84–95%] and 91% [79–98%]. In the field, the Dengue NS1 RDT sensitivity and specificity was 82% [60–95%] and 75% [53–90%], respectively. The IgM and IgG test lines displayed sensitivities of 86% [42–100%] and 78% [64–88%], specificities of 85% [76–92%] and 55% [36–73%], respectively. These results demonstrate that RDTs are ideal for use in a context of high prevalence or outbreak setting and can be implemented in the absence of a confirmatory test for acute and convalescent patients. MDPI 2023-03-31 /pmc/articles/PMC10143717/ /pubmed/37112887 http://dx.doi.org/10.3390/v15040904 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ndiaye, Oumar
Woolston, Kevin
Gaye, Aboubacry
Loucoubar, Cheikh
Cocozza, Michael
Fall, Cheikh
Dia, Fatou
Adams, Emily R.
Samb, Marième
Camara, Diogop
Sadio, Bacary Djilocalisse
Diagne, Cheikh T.
Weidmann, Manfred
Faye, Oumar
Fitchett, Joseph R. A.
Sall, Amadou Alpha
Diagne, Cheikh Tidiane
Laboratory Evaluation and Field Testing of Dengue NS1 and IgM/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal
title Laboratory Evaluation and Field Testing of Dengue NS1 and IgM/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal
title_full Laboratory Evaluation and Field Testing of Dengue NS1 and IgM/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal
title_fullStr Laboratory Evaluation and Field Testing of Dengue NS1 and IgM/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal
title_full_unstemmed Laboratory Evaluation and Field Testing of Dengue NS1 and IgM/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal
title_short Laboratory Evaluation and Field Testing of Dengue NS1 and IgM/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal
title_sort laboratory evaluation and field testing of dengue ns1 and igm/igg rapid diagnostic tests in an epidemic context in senegal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143717/
https://www.ncbi.nlm.nih.gov/pubmed/37112887
http://dx.doi.org/10.3390/v15040904
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