Hepatic Coccidiosis in Wild Rabbits in Greece: Parasite Detection on Liver Imprints and the Associated Biochemical Profile

SIMPLE SUMMARY: Hepatic coccidiosis is a severe rabbit parasitic disease caused by Eimeria stiedae. It may be fatal for rabbits and leads to important economic losses. The disease is well described in domestic rabbits, but little is known about infection with E. stiedae in wild rabbits. In this study, we investigated (a) whether wild rabbits from the island of Lemnos, Greece, where this species is overpopulated, are infected with E. stiedae and (b) what the effects are of this infection on their liver function. Based on our findings, 13.3% of the wild rabbits included in this study were infected with E. stiedae. Moreover, we observed several alterations in the liver variables in infected individuals compared to the non-infected ones, which were indicative of the negative effects of E. stiedae infection on the liver function of wild rabbits. This study adds to the current knowledge on the pathogens affecting wild rabbits and those circulating in this population on the island of Lemnos, Greece, and shows that E. stiedae infection impairs liver function in wild rabbits. ABSTRACT: (1) Background: Rabbit hepatic coccidiosis, caused by Eimeria stiedae, is a devastating disease with high morbidity and mortality rates. The disease is well described in rabbits, but little is known about E. stiedae infection in wild rabbits. In this study, we investigated the presence of E. stiedae infection in wild rabbits from the island of Lemnos, Greece, where this species is overpopulated, and the effects of infection on common hepatic biomarkers. (2) Methods: We used liver impression smears to detect the coccidian oocysts, and we defined the liver biochemical profile of the infected individuals. (3) Results: Overall, 13.3% of the liver imprints examined were positive for the presence of coccidial oocysts. The activities of liver enzymes, that is, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and glutamyltransferase (GGT), as well as globulins (GLOB), were increased while the concentrations of albumins (ALB), total proteins (TP) and the albumin to globulin (A/G) ratio were decreased in the infected individuals compared to the non-infected ones. (4) Conclusions: This study adds to the current knowledge on the pathogens affecting wild rabbits and those circulating in this population on the island of Lemnos, Greece. Moreover, we showed that E. stiedae infection exerts pathological effects on the hepatocyte integrity and liver function of wild rabbits, as reflected by the abnormal values of liver injury and dysfunction biomarkers.