Cargando…
Proteomics of Vespa velutina nigrithorax Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis
Health risks caused by stings from Vespa velutina nigrithorax (VV), also known as the yellow-legged Asian hornet, have become a public concern, but little is known about its venom composition. This study presents the proteome profile of the VV’s venom sac (VS) based on Sequential Window Acquisition...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144020/ https://www.ncbi.nlm.nih.gov/pubmed/37104204 http://dx.doi.org/10.3390/toxins15040266 |
_version_ | 1785034002766233600 |
---|---|
author | Alonso-Sampedro, Manuela Feás, Xesús Bravo, Susana Belén Chantada-Vázquez, María Pilar Vidal, Carmen |
author_facet | Alonso-Sampedro, Manuela Feás, Xesús Bravo, Susana Belén Chantada-Vázquez, María Pilar Vidal, Carmen |
author_sort | Alonso-Sampedro, Manuela |
collection | PubMed |
description | Health risks caused by stings from Vespa velutina nigrithorax (VV), also known as the yellow-legged Asian hornet, have become a public concern, but little is known about its venom composition. This study presents the proteome profile of the VV’s venom sac (VS) based on Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS). The study also performed proteomic quantitative analysis and examined the biological pathways and molecular functions of the proteins in the VS of VV gynes (i.e., future queens [SQ]) and workers [SW]. The total protein content per VS was significantly higher in the SW than in the SQ (274 ± 54 µg/sac vs. 175 ± 22 µg/sac; p = 0.02). We quantified a total of 228 proteins in the VS, belonging to 7 different classes: Insecta (n = 191); Amphibia and Reptilia (n = 20); Bacilli, γ-Proteobacteria and Pisoniviricetes (n = 12); and Arachnida (n = 5). Among the 228 identified proteins, 66 showed significant differential expression between SQ and SW. The potential allergens hyaluronidase A, venom antigen 5 and phospholipase A1 were significantly downregulated in the SQ venom. |
format | Online Article Text |
id | pubmed-10144020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101440202023-04-29 Proteomics of Vespa velutina nigrithorax Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis Alonso-Sampedro, Manuela Feás, Xesús Bravo, Susana Belén Chantada-Vázquez, María Pilar Vidal, Carmen Toxins (Basel) Article Health risks caused by stings from Vespa velutina nigrithorax (VV), also known as the yellow-legged Asian hornet, have become a public concern, but little is known about its venom composition. This study presents the proteome profile of the VV’s venom sac (VS) based on Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS). The study also performed proteomic quantitative analysis and examined the biological pathways and molecular functions of the proteins in the VS of VV gynes (i.e., future queens [SQ]) and workers [SW]. The total protein content per VS was significantly higher in the SW than in the SQ (274 ± 54 µg/sac vs. 175 ± 22 µg/sac; p = 0.02). We quantified a total of 228 proteins in the VS, belonging to 7 different classes: Insecta (n = 191); Amphibia and Reptilia (n = 20); Bacilli, γ-Proteobacteria and Pisoniviricetes (n = 12); and Arachnida (n = 5). Among the 228 identified proteins, 66 showed significant differential expression between SQ and SW. The potential allergens hyaluronidase A, venom antigen 5 and phospholipase A1 were significantly downregulated in the SQ venom. MDPI 2023-04-03 /pmc/articles/PMC10144020/ /pubmed/37104204 http://dx.doi.org/10.3390/toxins15040266 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alonso-Sampedro, Manuela Feás, Xesús Bravo, Susana Belén Chantada-Vázquez, María Pilar Vidal, Carmen Proteomics of Vespa velutina nigrithorax Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
title | Proteomics of Vespa velutina nigrithorax Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
title_full | Proteomics of Vespa velutina nigrithorax Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
title_fullStr | Proteomics of Vespa velutina nigrithorax Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
title_full_unstemmed | Proteomics of Vespa velutina nigrithorax Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
title_short | Proteomics of Vespa velutina nigrithorax Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis |
title_sort | proteomics of vespa velutina nigrithorax venom sac queens and workers: a quantitative swath-ms analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144020/ https://www.ncbi.nlm.nih.gov/pubmed/37104204 http://dx.doi.org/10.3390/toxins15040266 |
work_keys_str_mv | AT alonsosampedromanuela proteomicsofvespavelutinanigrithoraxvenomsacqueensandworkersaquantitativeswathmsanalysis AT feasxesus proteomicsofvespavelutinanigrithoraxvenomsacqueensandworkersaquantitativeswathmsanalysis AT bravosusanabelen proteomicsofvespavelutinanigrithoraxvenomsacqueensandworkersaquantitativeswathmsanalysis AT chantadavazquezmariapilar proteomicsofvespavelutinanigrithoraxvenomsacqueensandworkersaquantitativeswathmsanalysis AT vidalcarmen proteomicsofvespavelutinanigrithoraxvenomsacqueensandworkersaquantitativeswathmsanalysis |