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Portable Surface Plasmon Resonance Detector for COVID-19 Infection

Methods based on nucleic acid detection are currently the most commonly used technique in COVID-19 diagnostics. Although generally considered adequate, these methods are characterised by quite a long time-to-result and the necessity to prepare the material taken from the examined person—RNA isolatio...

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Autores principales: Trzaskowski, Maciej, Mazurkiewicz-Pisarek, Anna, Trzciński, Jakub Waldemar, Drozd, Marcin, Podgórski, Rafał, Zabost, Anna, Augustynowicz-Kopeć, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144119/
https://www.ncbi.nlm.nih.gov/pubmed/37112287
http://dx.doi.org/10.3390/s23083946
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author Trzaskowski, Maciej
Mazurkiewicz-Pisarek, Anna
Trzciński, Jakub Waldemar
Drozd, Marcin
Podgórski, Rafał
Zabost, Anna
Augustynowicz-Kopeć, Ewa
author_facet Trzaskowski, Maciej
Mazurkiewicz-Pisarek, Anna
Trzciński, Jakub Waldemar
Drozd, Marcin
Podgórski, Rafał
Zabost, Anna
Augustynowicz-Kopeć, Ewa
author_sort Trzaskowski, Maciej
collection PubMed
description Methods based on nucleic acid detection are currently the most commonly used technique in COVID-19 diagnostics. Although generally considered adequate, these methods are characterised by quite a long time-to-result and the necessity to prepare the material taken from the examined person—RNA isolation. For this reason, new detection methods are being sought, especially those characterised by the high speed of the analysis process from the moment of sampling to the result. Currently, serological methods of detecting antibodies against the virus in the patient’s blood plasma have attracted much attention. Although they are less precise in determining the current infection, such methods shorten the analysis time to several minutes, making it possible to consider them a promising method for screening tests in people with suspected infection. The described study investigated the feasibility of a surface plasmon resonance (SPR)-based detection system for on-site COVID-19 diagnostics. A simple-to-use portable device was proposed for the fast detection of anti-SARS-CoV-2 antibodies in human plasma. SARS-CoV-2-positive and -negative patient blood plasma samples were investigated and compared with the ELISA test. The receptor-binding domain (RBD) of spike protein from SARS-CoV-2 was selected as a binding molecule for the study. Then, the process of antibody detection using this peptide was examined under laboratory conditions on a commercially available SPR device. The portable device was prepared and tested on plasma samples from humans. The results were compared with those obtained in the same patients using the reference diagnostic method. The detection system is effective in the detection of anti-SARS-CoV-2 with the detection limit of 40 ng/mL. It was shown that it is a portable device that can correctly examine human plasma samples within a 10 min timeframe.
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spelling pubmed-101441192023-04-29 Portable Surface Plasmon Resonance Detector for COVID-19 Infection Trzaskowski, Maciej Mazurkiewicz-Pisarek, Anna Trzciński, Jakub Waldemar Drozd, Marcin Podgórski, Rafał Zabost, Anna Augustynowicz-Kopeć, Ewa Sensors (Basel) Article Methods based on nucleic acid detection are currently the most commonly used technique in COVID-19 diagnostics. Although generally considered adequate, these methods are characterised by quite a long time-to-result and the necessity to prepare the material taken from the examined person—RNA isolation. For this reason, new detection methods are being sought, especially those characterised by the high speed of the analysis process from the moment of sampling to the result. Currently, serological methods of detecting antibodies against the virus in the patient’s blood plasma have attracted much attention. Although they are less precise in determining the current infection, such methods shorten the analysis time to several minutes, making it possible to consider them a promising method for screening tests in people with suspected infection. The described study investigated the feasibility of a surface plasmon resonance (SPR)-based detection system for on-site COVID-19 diagnostics. A simple-to-use portable device was proposed for the fast detection of anti-SARS-CoV-2 antibodies in human plasma. SARS-CoV-2-positive and -negative patient blood plasma samples were investigated and compared with the ELISA test. The receptor-binding domain (RBD) of spike protein from SARS-CoV-2 was selected as a binding molecule for the study. Then, the process of antibody detection using this peptide was examined under laboratory conditions on a commercially available SPR device. The portable device was prepared and tested on plasma samples from humans. The results were compared with those obtained in the same patients using the reference diagnostic method. The detection system is effective in the detection of anti-SARS-CoV-2 with the detection limit of 40 ng/mL. It was shown that it is a portable device that can correctly examine human plasma samples within a 10 min timeframe. MDPI 2023-04-13 /pmc/articles/PMC10144119/ /pubmed/37112287 http://dx.doi.org/10.3390/s23083946 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trzaskowski, Maciej
Mazurkiewicz-Pisarek, Anna
Trzciński, Jakub Waldemar
Drozd, Marcin
Podgórski, Rafał
Zabost, Anna
Augustynowicz-Kopeć, Ewa
Portable Surface Plasmon Resonance Detector for COVID-19 Infection
title Portable Surface Plasmon Resonance Detector for COVID-19 Infection
title_full Portable Surface Plasmon Resonance Detector for COVID-19 Infection
title_fullStr Portable Surface Plasmon Resonance Detector for COVID-19 Infection
title_full_unstemmed Portable Surface Plasmon Resonance Detector for COVID-19 Infection
title_short Portable Surface Plasmon Resonance Detector for COVID-19 Infection
title_sort portable surface plasmon resonance detector for covid-19 infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144119/
https://www.ncbi.nlm.nih.gov/pubmed/37112287
http://dx.doi.org/10.3390/s23083946
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