Protocol to Assess the Biological Activity of Insulin Glargine, Insulin Lispro, and Insulin Aspart In Vitro

Insulin is a hormone produced by β-cells of the pancreas and controls the amount of sugar in the blood. Since its discovery over 100 years ago, insulin has been used as a life-saving treatment for people with diabetes. Historically, the biological activity or bioidentity of insulin products has been...

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Autores principales: Garige, Mamatha, Ghosh, Susmita, Roelofs, Brian, Rao, V. Ashutosh, Sourbier, Carole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144404/
https://www.ncbi.nlm.nih.gov/pubmed/37104015
http://dx.doi.org/10.3390/mps6020033
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author Garige, Mamatha
Ghosh, Susmita
Roelofs, Brian
Rao, V. Ashutosh
Sourbier, Carole
author_facet Garige, Mamatha
Ghosh, Susmita
Roelofs, Brian
Rao, V. Ashutosh
Sourbier, Carole
author_sort Garige, Mamatha
collection PubMed
description Insulin is a hormone produced by β-cells of the pancreas and controls the amount of sugar in the blood. Since its discovery over 100 years ago, insulin has been used as a life-saving treatment for people with diabetes. Historically, the biological activity or bioidentity of insulin products has been assessed using an in vivo model. However, reduction in animal experiments is a goal for many worldwide, and there is a need to develop in vitro bioassays to reliably test the biological activity of insulin products. This article describes an in vitro cell-based method to assess the biological activity of insulin glargine, insulin aspart, and insulin lispro in a step-by-step manner.
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spelling pubmed-101444042023-04-29 Protocol to Assess the Biological Activity of Insulin Glargine, Insulin Lispro, and Insulin Aspart In Vitro Garige, Mamatha Ghosh, Susmita Roelofs, Brian Rao, V. Ashutosh Sourbier, Carole Methods Protoc Protocol Insulin is a hormone produced by β-cells of the pancreas and controls the amount of sugar in the blood. Since its discovery over 100 years ago, insulin has been used as a life-saving treatment for people with diabetes. Historically, the biological activity or bioidentity of insulin products has been assessed using an in vivo model. However, reduction in animal experiments is a goal for many worldwide, and there is a need to develop in vitro bioassays to reliably test the biological activity of insulin products. This article describes an in vitro cell-based method to assess the biological activity of insulin glargine, insulin aspart, and insulin lispro in a step-by-step manner. MDPI 2023-03-24 /pmc/articles/PMC10144404/ /pubmed/37104015 http://dx.doi.org/10.3390/mps6020033 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Garige, Mamatha
Ghosh, Susmita
Roelofs, Brian
Rao, V. Ashutosh
Sourbier, Carole
Protocol to Assess the Biological Activity of Insulin Glargine, Insulin Lispro, and Insulin Aspart In Vitro
title Protocol to Assess the Biological Activity of Insulin Glargine, Insulin Lispro, and Insulin Aspart In Vitro
title_full Protocol to Assess the Biological Activity of Insulin Glargine, Insulin Lispro, and Insulin Aspart In Vitro
title_fullStr Protocol to Assess the Biological Activity of Insulin Glargine, Insulin Lispro, and Insulin Aspart In Vitro
title_full_unstemmed Protocol to Assess the Biological Activity of Insulin Glargine, Insulin Lispro, and Insulin Aspart In Vitro
title_short Protocol to Assess the Biological Activity of Insulin Glargine, Insulin Lispro, and Insulin Aspart In Vitro
title_sort protocol to assess the biological activity of insulin glargine, insulin lispro, and insulin aspart in vitro
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144404/
https://www.ncbi.nlm.nih.gov/pubmed/37104015
http://dx.doi.org/10.3390/mps6020033
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