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Poly(vinyl alcohol)/Silk Fibroin/Ag-NPs Composite Nanofibers as a Substrate for MG-63 Cells’ Growth

Nanofiber scaffolds of polyvinyl alcohol, silk fibroin from Bombyx mori cocoons, and silver nanoparticles were developed as a substrate for MG-63 growth. The fiber morphology, mechanical properties, thermal degradation, chemical composition, and water contact angle were investigated. In vitro tests...

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Autores principales: Mejía Suaza, Monica L., Leos Rivera, Jennifer C., Rodríguez Padilla, Maria C., Moncada Acevedo, Maria E., Ossa Orozco, Claudia P., Zarate Triviño, Diana G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144439/
https://www.ncbi.nlm.nih.gov/pubmed/37111985
http://dx.doi.org/10.3390/polym15081838
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author Mejía Suaza, Monica L.
Leos Rivera, Jennifer C.
Rodríguez Padilla, Maria C.
Moncada Acevedo, Maria E.
Ossa Orozco, Claudia P.
Zarate Triviño, Diana G.
author_facet Mejía Suaza, Monica L.
Leos Rivera, Jennifer C.
Rodríguez Padilla, Maria C.
Moncada Acevedo, Maria E.
Ossa Orozco, Claudia P.
Zarate Triviño, Diana G.
author_sort Mejía Suaza, Monica L.
collection PubMed
description Nanofiber scaffolds of polyvinyl alcohol, silk fibroin from Bombyx mori cocoons, and silver nanoparticles were developed as a substrate for MG-63 growth. The fiber morphology, mechanical properties, thermal degradation, chemical composition, and water contact angle were investigated. In vitro tests were performed by the cell viability MTS test of MG-63 cells on electrospun PVA scaffolds, mineralization was analyzed by alizarin red, and the alkaline phosphatase (ALP) assay was evaluated. At higher PVA concentrations, Young’s modulus (E) increased. The addition of fibroin and silver nanoparticles improved the thermal stability of PVA scaffolds. FTIR spectra indicated characteristic absorption peaks related to the chemical structures of PVA, fibroin, and Ag-NPs, demonstrating good interactions between them. The contact angle of the PVA scaffolds decreased with the incorporation of fibroin and showed hydrophilic characteristics. In all concentrations, MG-63 cells on PVA/fibroin/Ag-NPs scaffolds had higher cell viability than PVA pristine. On day ten of culture, PVA18/SF/Ag-NPs showed the highest mineralization, observed by the alizarin red test. PVA10/SF/Ag-NPs presented the highest alkaline phosphatase activity after an incubation time of 37 h. The achievements indicate the potential of the nanofibers of PVA18/SF/Ag-NPs as a possible substitute for bone tissue engineering (BTE).
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spelling pubmed-101444392023-04-29 Poly(vinyl alcohol)/Silk Fibroin/Ag-NPs Composite Nanofibers as a Substrate for MG-63 Cells’ Growth Mejía Suaza, Monica L. Leos Rivera, Jennifer C. Rodríguez Padilla, Maria C. Moncada Acevedo, Maria E. Ossa Orozco, Claudia P. Zarate Triviño, Diana G. Polymers (Basel) Article Nanofiber scaffolds of polyvinyl alcohol, silk fibroin from Bombyx mori cocoons, and silver nanoparticles were developed as a substrate for MG-63 growth. The fiber morphology, mechanical properties, thermal degradation, chemical composition, and water contact angle were investigated. In vitro tests were performed by the cell viability MTS test of MG-63 cells on electrospun PVA scaffolds, mineralization was analyzed by alizarin red, and the alkaline phosphatase (ALP) assay was evaluated. At higher PVA concentrations, Young’s modulus (E) increased. The addition of fibroin and silver nanoparticles improved the thermal stability of PVA scaffolds. FTIR spectra indicated characteristic absorption peaks related to the chemical structures of PVA, fibroin, and Ag-NPs, demonstrating good interactions between them. The contact angle of the PVA scaffolds decreased with the incorporation of fibroin and showed hydrophilic characteristics. In all concentrations, MG-63 cells on PVA/fibroin/Ag-NPs scaffolds had higher cell viability than PVA pristine. On day ten of culture, PVA18/SF/Ag-NPs showed the highest mineralization, observed by the alizarin red test. PVA10/SF/Ag-NPs presented the highest alkaline phosphatase activity after an incubation time of 37 h. The achievements indicate the potential of the nanofibers of PVA18/SF/Ag-NPs as a possible substitute for bone tissue engineering (BTE). MDPI 2023-04-11 /pmc/articles/PMC10144439/ /pubmed/37111985 http://dx.doi.org/10.3390/polym15081838 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mejía Suaza, Monica L.
Leos Rivera, Jennifer C.
Rodríguez Padilla, Maria C.
Moncada Acevedo, Maria E.
Ossa Orozco, Claudia P.
Zarate Triviño, Diana G.
Poly(vinyl alcohol)/Silk Fibroin/Ag-NPs Composite Nanofibers as a Substrate for MG-63 Cells’ Growth
title Poly(vinyl alcohol)/Silk Fibroin/Ag-NPs Composite Nanofibers as a Substrate for MG-63 Cells’ Growth
title_full Poly(vinyl alcohol)/Silk Fibroin/Ag-NPs Composite Nanofibers as a Substrate for MG-63 Cells’ Growth
title_fullStr Poly(vinyl alcohol)/Silk Fibroin/Ag-NPs Composite Nanofibers as a Substrate for MG-63 Cells’ Growth
title_full_unstemmed Poly(vinyl alcohol)/Silk Fibroin/Ag-NPs Composite Nanofibers as a Substrate for MG-63 Cells’ Growth
title_short Poly(vinyl alcohol)/Silk Fibroin/Ag-NPs Composite Nanofibers as a Substrate for MG-63 Cells’ Growth
title_sort poly(vinyl alcohol)/silk fibroin/ag-nps composite nanofibers as a substrate for mg-63 cells’ growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144439/
https://www.ncbi.nlm.nih.gov/pubmed/37111985
http://dx.doi.org/10.3390/polym15081838
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