Cargando…

Novel Bovine Serum Albumin-Decorated–Nanostructured Lipid Carriers Able to Modulate Apoptosis and Cell-Cycle Response in Ovarian, Breast, and Colon Tumoral Cells

A novel nanoscale approach was developed for the improved cellular internalization of hybrid bovine serum albumin–lipid nanocarriers loaded with piperine (NLC-Pip–BSA) in different tumor cells. The effect of the BSA-targeted–NLC-Pip and untargeted-NLC-Pip on the viability, proliferation, and levels...

Descripción completa

Detalles Bibliográficos
Autores principales: Tincu, Robert, Mihaila, Mirela, Bostan, Marinela, Teodorescu, Florina, Istrati, Daniela, Badea, Nicoleta, Lacatusu, Ioana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144507/
https://www.ncbi.nlm.nih.gov/pubmed/37111611
http://dx.doi.org/10.3390/pharmaceutics15041125
_version_ 1785034116495835136
author Tincu, Robert
Mihaila, Mirela
Bostan, Marinela
Teodorescu, Florina
Istrati, Daniela
Badea, Nicoleta
Lacatusu, Ioana
author_facet Tincu, Robert
Mihaila, Mirela
Bostan, Marinela
Teodorescu, Florina
Istrati, Daniela
Badea, Nicoleta
Lacatusu, Ioana
author_sort Tincu, Robert
collection PubMed
description A novel nanoscale approach was developed for the improved cellular internalization of hybrid bovine serum albumin–lipid nanocarriers loaded with piperine (NLC-Pip–BSA) in different tumor cells. The effect of the BSA-targeted–NLC-Pip and untargeted-NLC-Pip on the viability, proliferation, and levels of cell-cycle damage and apoptosis in the colon (LoVo), ovarian (SKOV3) and breast (MCF7) adenocarcinoma cell lines was comparatively discussed. NLCs were characterized concerning particle size, morphology, zeta potential, phytochemical encapsulation efficiency, ATR-FTIR, and fluorescence spectroscopy. The results showed that NLC-Pip–BSA showed a mean size below 140 nm, a zeta potential of −60 mV, and an entrapment efficiency of 81.94% for NLC-Pip and 80.45% for NLC-Pip–BSA. Fluorescence spectroscopy confirmed the coating of the NLC with the albumin. By MTS and RTCA assays, NLC-Pip–BSA showed a more pronounced response against the LoVo colon cell line and MCF-7 breast tumor cell lines than against the ovarian SKOV-3 cell line. Flow cytometry assay demonstrated that the targeted NLC-Pip had more cytotoxicity and improved apoptosis than the untargeted ones in MCF-7 tumor cells (p < 0.05). NLC-Pip caused a significant increase in MCF-7 breast tumor cell apoptosis of ~8X, while NLC-Pip–BSA has shown an 11-fold increase in apoptosis.
format Online
Article
Text
id pubmed-10144507
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101445072023-04-29 Novel Bovine Serum Albumin-Decorated–Nanostructured Lipid Carriers Able to Modulate Apoptosis and Cell-Cycle Response in Ovarian, Breast, and Colon Tumoral Cells Tincu, Robert Mihaila, Mirela Bostan, Marinela Teodorescu, Florina Istrati, Daniela Badea, Nicoleta Lacatusu, Ioana Pharmaceutics Article A novel nanoscale approach was developed for the improved cellular internalization of hybrid bovine serum albumin–lipid nanocarriers loaded with piperine (NLC-Pip–BSA) in different tumor cells. The effect of the BSA-targeted–NLC-Pip and untargeted-NLC-Pip on the viability, proliferation, and levels of cell-cycle damage and apoptosis in the colon (LoVo), ovarian (SKOV3) and breast (MCF7) adenocarcinoma cell lines was comparatively discussed. NLCs were characterized concerning particle size, morphology, zeta potential, phytochemical encapsulation efficiency, ATR-FTIR, and fluorescence spectroscopy. The results showed that NLC-Pip–BSA showed a mean size below 140 nm, a zeta potential of −60 mV, and an entrapment efficiency of 81.94% for NLC-Pip and 80.45% for NLC-Pip–BSA. Fluorescence spectroscopy confirmed the coating of the NLC with the albumin. By MTS and RTCA assays, NLC-Pip–BSA showed a more pronounced response against the LoVo colon cell line and MCF-7 breast tumor cell lines than against the ovarian SKOV-3 cell line. Flow cytometry assay demonstrated that the targeted NLC-Pip had more cytotoxicity and improved apoptosis than the untargeted ones in MCF-7 tumor cells (p < 0.05). NLC-Pip caused a significant increase in MCF-7 breast tumor cell apoptosis of ~8X, while NLC-Pip–BSA has shown an 11-fold increase in apoptosis. MDPI 2023-04-02 /pmc/articles/PMC10144507/ /pubmed/37111611 http://dx.doi.org/10.3390/pharmaceutics15041125 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tincu, Robert
Mihaila, Mirela
Bostan, Marinela
Teodorescu, Florina
Istrati, Daniela
Badea, Nicoleta
Lacatusu, Ioana
Novel Bovine Serum Albumin-Decorated–Nanostructured Lipid Carriers Able to Modulate Apoptosis and Cell-Cycle Response in Ovarian, Breast, and Colon Tumoral Cells
title Novel Bovine Serum Albumin-Decorated–Nanostructured Lipid Carriers Able to Modulate Apoptosis and Cell-Cycle Response in Ovarian, Breast, and Colon Tumoral Cells
title_full Novel Bovine Serum Albumin-Decorated–Nanostructured Lipid Carriers Able to Modulate Apoptosis and Cell-Cycle Response in Ovarian, Breast, and Colon Tumoral Cells
title_fullStr Novel Bovine Serum Albumin-Decorated–Nanostructured Lipid Carriers Able to Modulate Apoptosis and Cell-Cycle Response in Ovarian, Breast, and Colon Tumoral Cells
title_full_unstemmed Novel Bovine Serum Albumin-Decorated–Nanostructured Lipid Carriers Able to Modulate Apoptosis and Cell-Cycle Response in Ovarian, Breast, and Colon Tumoral Cells
title_short Novel Bovine Serum Albumin-Decorated–Nanostructured Lipid Carriers Able to Modulate Apoptosis and Cell-Cycle Response in Ovarian, Breast, and Colon Tumoral Cells
title_sort novel bovine serum albumin-decorated–nanostructured lipid carriers able to modulate apoptosis and cell-cycle response in ovarian, breast, and colon tumoral cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144507/
https://www.ncbi.nlm.nih.gov/pubmed/37111611
http://dx.doi.org/10.3390/pharmaceutics15041125
work_keys_str_mv AT tincurobert novelbovineserumalbumindecoratednanostructuredlipidcarriersabletomodulateapoptosisandcellcycleresponseinovarianbreastandcolontumoralcells
AT mihailamirela novelbovineserumalbumindecoratednanostructuredlipidcarriersabletomodulateapoptosisandcellcycleresponseinovarianbreastandcolontumoralcells
AT bostanmarinela novelbovineserumalbumindecoratednanostructuredlipidcarriersabletomodulateapoptosisandcellcycleresponseinovarianbreastandcolontumoralcells
AT teodorescuflorina novelbovineserumalbumindecoratednanostructuredlipidcarriersabletomodulateapoptosisandcellcycleresponseinovarianbreastandcolontumoralcells
AT istratidaniela novelbovineserumalbumindecoratednanostructuredlipidcarriersabletomodulateapoptosisandcellcycleresponseinovarianbreastandcolontumoralcells
AT badeanicoleta novelbovineserumalbumindecoratednanostructuredlipidcarriersabletomodulateapoptosisandcellcycleresponseinovarianbreastandcolontumoralcells
AT lacatusuioana novelbovineserumalbumindecoratednanostructuredlipidcarriersabletomodulateapoptosisandcellcycleresponseinovarianbreastandcolontumoralcells