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Stability and Thrombogenicity Analysis of Collagen/Carbon Nanotube Nanocomposite Coatings Using a Reversible Microfluidic Device

Currently, the development of stable and antithrombogenic coatings for cardiovascular implants is socially important. This is especially important for coatings exposed to high shear stress from flowing blood, such as those on ventricular assist devices. A method of layer-by-layer formation of nanoco...

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Autores principales: Popovich, Kristina D., Vagner, Sergey A., Murashko, Denis T., Ten, Galina N., Ryabkin, Dmitry I., Savelyev, Mikhail S., Kitsyuk, Evgeny P., Gerasimenko, Ekaterina A., Edelbekova, Polina, Konovalov, Anton N., Telyshev, Dmitry V., Selishchev, Sergey V., Gerasimenko, Alexander Yu.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144663/
https://www.ncbi.nlm.nih.gov/pubmed/37103830
http://dx.doi.org/10.3390/membranes13040403
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author Popovich, Kristina D.
Vagner, Sergey A.
Murashko, Denis T.
Ten, Galina N.
Ryabkin, Dmitry I.
Savelyev, Mikhail S.
Kitsyuk, Evgeny P.
Gerasimenko, Ekaterina A.
Edelbekova, Polina
Konovalov, Anton N.
Telyshev, Dmitry V.
Selishchev, Sergey V.
Gerasimenko, Alexander Yu.
author_facet Popovich, Kristina D.
Vagner, Sergey A.
Murashko, Denis T.
Ten, Galina N.
Ryabkin, Dmitry I.
Savelyev, Mikhail S.
Kitsyuk, Evgeny P.
Gerasimenko, Ekaterina A.
Edelbekova, Polina
Konovalov, Anton N.
Telyshev, Dmitry V.
Selishchev, Sergey V.
Gerasimenko, Alexander Yu.
author_sort Popovich, Kristina D.
collection PubMed
description Currently, the development of stable and antithrombogenic coatings for cardiovascular implants is socially important. This is especially important for coatings exposed to high shear stress from flowing blood, such as those on ventricular assist devices. A method of layer-by-layer formation of nanocomposite coatings based on multi-walled carbon nanotubes (MWCNT) in a collagen matrix is proposed. A reversible microfluidic device with a wide range of flow shear stresses has been developed for hemodynamic experiments. The dependence of the resistance on the presence of a cross-linking agent for collagen chains in the composition of the coating was demonstrated. Optical profilometry determined that collagen/c-MWCNT and collagen/c-MWCNT/glutaraldehyde coatings obtained sufficiently high resistance to high shear stress flow. However, the collagen/c-MWCNT/glutaraldehyde coating was almost twice as resistant to a phosphate-buffered solution flow. A reversible microfluidic device made it possible to assess the level of thrombogenicity of the coatings by the level of blood albumin protein adhesion to the coatings. Raman spectroscopy demonstrated that the adhesion of albumin to collagen/c-MWCNT and collagen/c-MWCNT/glutaraldehyde coatings is 1.7 and 1.4 times lower than the adhesion of protein to a titanium surface, widely used for ventricular assist devices. Scanning electron microscopy and energy dispersive spectroscopy determined that blood protein was least detected on the collagen/c-MWCNT coating, which contained no cross-linking agent, including in comparison with the titanium surface. Thus, a reversible microfluidic device is suitable for preliminary testing of the resistance and thrombogenicity of various coatings and membranes, and nanocomposite coatings based on collagen and c-MWCNT are suitable candidates for the development of cardiovascular devices.
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spelling pubmed-101446632023-04-29 Stability and Thrombogenicity Analysis of Collagen/Carbon Nanotube Nanocomposite Coatings Using a Reversible Microfluidic Device Popovich, Kristina D. Vagner, Sergey A. Murashko, Denis T. Ten, Galina N. Ryabkin, Dmitry I. Savelyev, Mikhail S. Kitsyuk, Evgeny P. Gerasimenko, Ekaterina A. Edelbekova, Polina Konovalov, Anton N. Telyshev, Dmitry V. Selishchev, Sergey V. Gerasimenko, Alexander Yu. Membranes (Basel) Article Currently, the development of stable and antithrombogenic coatings for cardiovascular implants is socially important. This is especially important for coatings exposed to high shear stress from flowing blood, such as those on ventricular assist devices. A method of layer-by-layer formation of nanocomposite coatings based on multi-walled carbon nanotubes (MWCNT) in a collagen matrix is proposed. A reversible microfluidic device with a wide range of flow shear stresses has been developed for hemodynamic experiments. The dependence of the resistance on the presence of a cross-linking agent for collagen chains in the composition of the coating was demonstrated. Optical profilometry determined that collagen/c-MWCNT and collagen/c-MWCNT/glutaraldehyde coatings obtained sufficiently high resistance to high shear stress flow. However, the collagen/c-MWCNT/glutaraldehyde coating was almost twice as resistant to a phosphate-buffered solution flow. A reversible microfluidic device made it possible to assess the level of thrombogenicity of the coatings by the level of blood albumin protein adhesion to the coatings. Raman spectroscopy demonstrated that the adhesion of albumin to collagen/c-MWCNT and collagen/c-MWCNT/glutaraldehyde coatings is 1.7 and 1.4 times lower than the adhesion of protein to a titanium surface, widely used for ventricular assist devices. Scanning electron microscopy and energy dispersive spectroscopy determined that blood protein was least detected on the collagen/c-MWCNT coating, which contained no cross-linking agent, including in comparison with the titanium surface. Thus, a reversible microfluidic device is suitable for preliminary testing of the resistance and thrombogenicity of various coatings and membranes, and nanocomposite coatings based on collagen and c-MWCNT are suitable candidates for the development of cardiovascular devices. MDPI 2023-04-01 /pmc/articles/PMC10144663/ /pubmed/37103830 http://dx.doi.org/10.3390/membranes13040403 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Popovich, Kristina D.
Vagner, Sergey A.
Murashko, Denis T.
Ten, Galina N.
Ryabkin, Dmitry I.
Savelyev, Mikhail S.
Kitsyuk, Evgeny P.
Gerasimenko, Ekaterina A.
Edelbekova, Polina
Konovalov, Anton N.
Telyshev, Dmitry V.
Selishchev, Sergey V.
Gerasimenko, Alexander Yu.
Stability and Thrombogenicity Analysis of Collagen/Carbon Nanotube Nanocomposite Coatings Using a Reversible Microfluidic Device
title Stability and Thrombogenicity Analysis of Collagen/Carbon Nanotube Nanocomposite Coatings Using a Reversible Microfluidic Device
title_full Stability and Thrombogenicity Analysis of Collagen/Carbon Nanotube Nanocomposite Coatings Using a Reversible Microfluidic Device
title_fullStr Stability and Thrombogenicity Analysis of Collagen/Carbon Nanotube Nanocomposite Coatings Using a Reversible Microfluidic Device
title_full_unstemmed Stability and Thrombogenicity Analysis of Collagen/Carbon Nanotube Nanocomposite Coatings Using a Reversible Microfluidic Device
title_short Stability and Thrombogenicity Analysis of Collagen/Carbon Nanotube Nanocomposite Coatings Using a Reversible Microfluidic Device
title_sort stability and thrombogenicity analysis of collagen/carbon nanotube nanocomposite coatings using a reversible microfluidic device
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144663/
https://www.ncbi.nlm.nih.gov/pubmed/37103830
http://dx.doi.org/10.3390/membranes13040403
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