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Reaction of Cornu aspersum Immune System against Different Aelurostrongylus abstrusus Developmental Stages

Cornu aspersum, the land snail, is recognized as a suitable intermediate host of Aelurostrongylus abstrusus; however, there is little information both on larval development as well as on the intermediate host’s immune system reaction to the parasite. The aim of the study was to assess the histologic...

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Autores principales: Napoli, Ettore, Sfacteria, Alessandra, Rifici, Claudia, Mazzullo, Giuseppe, Gaglio, Gabriella, Brianti, Emanuele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144845/
https://www.ncbi.nlm.nih.gov/pubmed/37111428
http://dx.doi.org/10.3390/pathogens12040542
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author Napoli, Ettore
Sfacteria, Alessandra
Rifici, Claudia
Mazzullo, Giuseppe
Gaglio, Gabriella
Brianti, Emanuele
author_facet Napoli, Ettore
Sfacteria, Alessandra
Rifici, Claudia
Mazzullo, Giuseppe
Gaglio, Gabriella
Brianti, Emanuele
author_sort Napoli, Ettore
collection PubMed
description Cornu aspersum, the land snail, is recognized as a suitable intermediate host of Aelurostrongylus abstrusus; however, there is little information both on larval development as well as on the intermediate host’s immune system reaction to the parasite. The aim of the study was to assess the histological reaction of C. aspersum’s immune system against A. abstrusus. Sixty-five snails were provided by a snail farm. Five of them were digested to assess the absence of natural parasitic infections. The remaining sixty were divided into five groups. Three groups of snails were infected with A. abstrusus using by-contact or injection methods; one group was injected only with saline solution and one group was left untreated as the control. The snails of group A were sacrificed and digested on study days 2, 10, and 18; snails of the other groups were collected and examined for histopathological analysis on study days 2, 10, and 18. On study day 2, in the infected snails, several free L1s were observed along with the absence of immune system reactions. On day 10, the L2s elicited an intense reaction in the internal layer of the muscular foot. On day 18, all L3s partially encapsulated by the snail’s immune system were observed in the outermost part of the muscular foot, which is near and among the goblet cells. This last finding suggests that L3s could be shed with the snail’s mucus and spread in the environment, representing an alternative route of transmission for this feline lungworm.
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spelling pubmed-101448452023-04-29 Reaction of Cornu aspersum Immune System against Different Aelurostrongylus abstrusus Developmental Stages Napoli, Ettore Sfacteria, Alessandra Rifici, Claudia Mazzullo, Giuseppe Gaglio, Gabriella Brianti, Emanuele Pathogens Article Cornu aspersum, the land snail, is recognized as a suitable intermediate host of Aelurostrongylus abstrusus; however, there is little information both on larval development as well as on the intermediate host’s immune system reaction to the parasite. The aim of the study was to assess the histological reaction of C. aspersum’s immune system against A. abstrusus. Sixty-five snails were provided by a snail farm. Five of them were digested to assess the absence of natural parasitic infections. The remaining sixty were divided into five groups. Three groups of snails were infected with A. abstrusus using by-contact or injection methods; one group was injected only with saline solution and one group was left untreated as the control. The snails of group A were sacrificed and digested on study days 2, 10, and 18; snails of the other groups were collected and examined for histopathological analysis on study days 2, 10, and 18. On study day 2, in the infected snails, several free L1s were observed along with the absence of immune system reactions. On day 10, the L2s elicited an intense reaction in the internal layer of the muscular foot. On day 18, all L3s partially encapsulated by the snail’s immune system were observed in the outermost part of the muscular foot, which is near and among the goblet cells. This last finding suggests that L3s could be shed with the snail’s mucus and spread in the environment, representing an alternative route of transmission for this feline lungworm. MDPI 2023-03-31 /pmc/articles/PMC10144845/ /pubmed/37111428 http://dx.doi.org/10.3390/pathogens12040542 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Napoli, Ettore
Sfacteria, Alessandra
Rifici, Claudia
Mazzullo, Giuseppe
Gaglio, Gabriella
Brianti, Emanuele
Reaction of Cornu aspersum Immune System against Different Aelurostrongylus abstrusus Developmental Stages
title Reaction of Cornu aspersum Immune System against Different Aelurostrongylus abstrusus Developmental Stages
title_full Reaction of Cornu aspersum Immune System against Different Aelurostrongylus abstrusus Developmental Stages
title_fullStr Reaction of Cornu aspersum Immune System against Different Aelurostrongylus abstrusus Developmental Stages
title_full_unstemmed Reaction of Cornu aspersum Immune System against Different Aelurostrongylus abstrusus Developmental Stages
title_short Reaction of Cornu aspersum Immune System against Different Aelurostrongylus abstrusus Developmental Stages
title_sort reaction of cornu aspersum immune system against different aelurostrongylus abstrusus developmental stages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144845/
https://www.ncbi.nlm.nih.gov/pubmed/37111428
http://dx.doi.org/10.3390/pathogens12040542
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