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Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods

Malaria is an infectious and parasitic disease caused by protozoa of the genus Plasmodium, which affects millions of people in tropical and subtropical areas. Recently, there have been multiple reports of drug resistance in Plasmodium populations, leading to the search for potential new active compo...

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Autores principales: de Assis, Francisco Flávio Vieira, de Almeida Junior, José Sousa, Moraes, Tânia Mara Pires, Varotti, Fernando de Pilla, Moraes, Camila Castilho, Sartoratto, Adilson, Moraes, Waldiney Pires, Minervino, Antonio Humberto Hamad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145024/
https://www.ncbi.nlm.nih.gov/pubmed/37111647
http://dx.doi.org/10.3390/pharmaceutics15041162
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author de Assis, Francisco Flávio Vieira
de Almeida Junior, José Sousa
Moraes, Tânia Mara Pires
Varotti, Fernando de Pilla
Moraes, Camila Castilho
Sartoratto, Adilson
Moraes, Waldiney Pires
Minervino, Antonio Humberto Hamad
author_facet de Assis, Francisco Flávio Vieira
de Almeida Junior, José Sousa
Moraes, Tânia Mara Pires
Varotti, Fernando de Pilla
Moraes, Camila Castilho
Sartoratto, Adilson
Moraes, Waldiney Pires
Minervino, Antonio Humberto Hamad
author_sort de Assis, Francisco Flávio Vieira
collection PubMed
description Malaria is an infectious and parasitic disease caused by protozoa of the genus Plasmodium, which affects millions of people in tropical and subtropical areas. Recently, there have been multiple reports of drug resistance in Plasmodium populations, leading to the search for potential new active compounds against the parasite. Thus, we aimed to evaluate the in vitro antiplasmodial activity and cytotoxicity of the hydroalcoholic extract of Jucá (Libidibia ferrea) in serial concentrations. Jucá was used in the form of a freeze-dried hydroalcoholic extract. For the cytotoxicity assay, the(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) method with the WI-26VA4 human cell line was used. For the antiplasmodial activity, Plasmodium falciparum synchronized cultures were treated with serial concentrations (0.2 to 50 μg/mL) of the Jucá extract. In terms of the chemical composition of the Jucá extract, gas chromatography coupled to mass spectrometry measurements revealed the main compounds as ellagic acid, valoneic acid dilactone, gallotannin, and gallic acid. The Jucá hydroalcoholic extract did not show cytotoxic activity per MTT, with an IC(50) value greater than 100 µg/mL. Regarding the antiplasmodial activity, the Jucá extract presented an IC(50) of 11.10 µg/mL with a selective index of nine. Because of its antiplasmodial activity at the tested concentrations and low toxicity, the Jucá extract is presented as a candidate for herbal medicine in the treatment of malaria. To the best of our knowledge, this is the first report of antiplasmodial activity in Jucá.
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spelling pubmed-101450242023-04-29 Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods de Assis, Francisco Flávio Vieira de Almeida Junior, José Sousa Moraes, Tânia Mara Pires Varotti, Fernando de Pilla Moraes, Camila Castilho Sartoratto, Adilson Moraes, Waldiney Pires Minervino, Antonio Humberto Hamad Pharmaceutics Article Malaria is an infectious and parasitic disease caused by protozoa of the genus Plasmodium, which affects millions of people in tropical and subtropical areas. Recently, there have been multiple reports of drug resistance in Plasmodium populations, leading to the search for potential new active compounds against the parasite. Thus, we aimed to evaluate the in vitro antiplasmodial activity and cytotoxicity of the hydroalcoholic extract of Jucá (Libidibia ferrea) in serial concentrations. Jucá was used in the form of a freeze-dried hydroalcoholic extract. For the cytotoxicity assay, the(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) method with the WI-26VA4 human cell line was used. For the antiplasmodial activity, Plasmodium falciparum synchronized cultures were treated with serial concentrations (0.2 to 50 μg/mL) of the Jucá extract. In terms of the chemical composition of the Jucá extract, gas chromatography coupled to mass spectrometry measurements revealed the main compounds as ellagic acid, valoneic acid dilactone, gallotannin, and gallic acid. The Jucá hydroalcoholic extract did not show cytotoxic activity per MTT, with an IC(50) value greater than 100 µg/mL. Regarding the antiplasmodial activity, the Jucá extract presented an IC(50) of 11.10 µg/mL with a selective index of nine. Because of its antiplasmodial activity at the tested concentrations and low toxicity, the Jucá extract is presented as a candidate for herbal medicine in the treatment of malaria. To the best of our knowledge, this is the first report of antiplasmodial activity in Jucá. MDPI 2023-04-06 /pmc/articles/PMC10145024/ /pubmed/37111647 http://dx.doi.org/10.3390/pharmaceutics15041162 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Assis, Francisco Flávio Vieira
de Almeida Junior, José Sousa
Moraes, Tânia Mara Pires
Varotti, Fernando de Pilla
Moraes, Camila Castilho
Sartoratto, Adilson
Moraes, Waldiney Pires
Minervino, Antonio Humberto Hamad
Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods
title Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods
title_full Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods
title_fullStr Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods
title_full_unstemmed Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods
title_short Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (Libidibia ferrea) Pods
title_sort antiplasmodial activity of hydroalcoholic extract from jucá (libidibia ferrea) pods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145024/
https://www.ncbi.nlm.nih.gov/pubmed/37111647
http://dx.doi.org/10.3390/pharmaceutics15041162
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