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A Wall Fragment of Cutibacterium acnes Preserves Junctional Integrity Altered by Staphylococcus aureus in an Ex Vivo Porcine Skin Model
(1) Background alteration of the skin microbiota, dysbiosis, causes skin barrier impairment resulting in disease development. Staphylococcus aureus, the main pathogen associated with dysbiosis, secretes several virulence factors, including α-toxin that damages tight junctions and compromises the int...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145065/ https://www.ncbi.nlm.nih.gov/pubmed/37111709 http://dx.doi.org/10.3390/pharmaceutics15041224 |
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author | Magnifico, Irene Perna, Angelica Cutuli, Marco Alfio Medoro, Alessandro Pietrangelo, Laura Guarnieri, Antonio Foderà, Emanuele Passarella, Daniela Venditti, Noemi Vergalito, Franca Petronio Petronio, Giulio Di Marco, Roberto |
author_facet | Magnifico, Irene Perna, Angelica Cutuli, Marco Alfio Medoro, Alessandro Pietrangelo, Laura Guarnieri, Antonio Foderà, Emanuele Passarella, Daniela Venditti, Noemi Vergalito, Franca Petronio Petronio, Giulio Di Marco, Roberto |
author_sort | Magnifico, Irene |
collection | PubMed |
description | (1) Background alteration of the skin microbiota, dysbiosis, causes skin barrier impairment resulting in disease development. Staphylococcus aureus, the main pathogen associated with dysbiosis, secretes several virulence factors, including α-toxin that damages tight junctions and compromises the integrity of the skin barrier. The use of members of the resident microbiota to restore the skin barrier, bacteriotherapy, represents a safe treatment for skin conditions among innovative options. The aim of this study is the evaluation of a wall fragment derived from a patented strain of Cutibacterium acnes DSM28251 (c40) alone and conjugated to a mucopolysaccharide carrier (HAc40) in counteracting S. aureus pathogenic action on two tight junction proteins (Claudin-1 and ZO-1) in an ex vivo porcine skin infection model. Methods: skin biopsies were infected with live S. aureus strains ATCC29213 and DSM20491. Tissue was pre-incubated or co-incubated with c40 and HAc40. (3) Results: c40 and HAc40 prevent and counteract Claudin-1 and Zo-1 damage (4) Conclusions: c40 and the functional ingredient HAc40 represent a potential non-pharmacological treatment of skin diseases associated with cutaneous dysbiosis of S. aureus. These findings offer numerous avenues for new research. |
format | Online Article Text |
id | pubmed-10145065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101450652023-04-29 A Wall Fragment of Cutibacterium acnes Preserves Junctional Integrity Altered by Staphylococcus aureus in an Ex Vivo Porcine Skin Model Magnifico, Irene Perna, Angelica Cutuli, Marco Alfio Medoro, Alessandro Pietrangelo, Laura Guarnieri, Antonio Foderà, Emanuele Passarella, Daniela Venditti, Noemi Vergalito, Franca Petronio Petronio, Giulio Di Marco, Roberto Pharmaceutics Article (1) Background alteration of the skin microbiota, dysbiosis, causes skin barrier impairment resulting in disease development. Staphylococcus aureus, the main pathogen associated with dysbiosis, secretes several virulence factors, including α-toxin that damages tight junctions and compromises the integrity of the skin barrier. The use of members of the resident microbiota to restore the skin barrier, bacteriotherapy, represents a safe treatment for skin conditions among innovative options. The aim of this study is the evaluation of a wall fragment derived from a patented strain of Cutibacterium acnes DSM28251 (c40) alone and conjugated to a mucopolysaccharide carrier (HAc40) in counteracting S. aureus pathogenic action on two tight junction proteins (Claudin-1 and ZO-1) in an ex vivo porcine skin infection model. Methods: skin biopsies were infected with live S. aureus strains ATCC29213 and DSM20491. Tissue was pre-incubated or co-incubated with c40 and HAc40. (3) Results: c40 and HAc40 prevent and counteract Claudin-1 and Zo-1 damage (4) Conclusions: c40 and the functional ingredient HAc40 represent a potential non-pharmacological treatment of skin diseases associated with cutaneous dysbiosis of S. aureus. These findings offer numerous avenues for new research. MDPI 2023-04-12 /pmc/articles/PMC10145065/ /pubmed/37111709 http://dx.doi.org/10.3390/pharmaceutics15041224 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Magnifico, Irene Perna, Angelica Cutuli, Marco Alfio Medoro, Alessandro Pietrangelo, Laura Guarnieri, Antonio Foderà, Emanuele Passarella, Daniela Venditti, Noemi Vergalito, Franca Petronio Petronio, Giulio Di Marco, Roberto A Wall Fragment of Cutibacterium acnes Preserves Junctional Integrity Altered by Staphylococcus aureus in an Ex Vivo Porcine Skin Model |
title | A Wall Fragment of Cutibacterium acnes Preserves Junctional Integrity Altered by Staphylococcus aureus in an Ex Vivo Porcine Skin Model |
title_full | A Wall Fragment of Cutibacterium acnes Preserves Junctional Integrity Altered by Staphylococcus aureus in an Ex Vivo Porcine Skin Model |
title_fullStr | A Wall Fragment of Cutibacterium acnes Preserves Junctional Integrity Altered by Staphylococcus aureus in an Ex Vivo Porcine Skin Model |
title_full_unstemmed | A Wall Fragment of Cutibacterium acnes Preserves Junctional Integrity Altered by Staphylococcus aureus in an Ex Vivo Porcine Skin Model |
title_short | A Wall Fragment of Cutibacterium acnes Preserves Junctional Integrity Altered by Staphylococcus aureus in an Ex Vivo Porcine Skin Model |
title_sort | wall fragment of cutibacterium acnes preserves junctional integrity altered by staphylococcus aureus in an ex vivo porcine skin model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145065/ https://www.ncbi.nlm.nih.gov/pubmed/37111709 http://dx.doi.org/10.3390/pharmaceutics15041224 |
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