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Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov
Nine novel bacterial strains were isolated from the feces of cats and sheep in 2019 and 2020 in Beijing, China. Cells were 1–3 μm long and ≤0.5 μm wide, Gram-stain negative, microaerobic, motile, oxidase positive, and urease negative. Phylogenetic analyses based on 16S rRNA gene sequences indicated...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145079/ https://www.ncbi.nlm.nih.gov/pubmed/37110394 http://dx.doi.org/10.3390/microorganisms11040971 |
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author | Wang, Hairui Li, Ying Gu, Yixin Zhou, Guilan Chen, Xiaoli Zhang, Xin Shao, Zhujun Zhang, Jianzhong Zhang, Maojun |
author_facet | Wang, Hairui Li, Ying Gu, Yixin Zhou, Guilan Chen, Xiaoli Zhang, Xin Shao, Zhujun Zhang, Jianzhong Zhang, Maojun |
author_sort | Wang, Hairui |
collection | PubMed |
description | Nine novel bacterial strains were isolated from the feces of cats and sheep in 2019 and 2020 in Beijing, China. Cells were 1–3 μm long and ≤0.5 μm wide, Gram-stain negative, microaerobic, motile, oxidase positive, and urease negative. Phylogenetic analyses based on 16S rRNA gene sequences indicated that these nine isolates belong to the genus Campylobacter but formed two robust clades that were clearly separate from the currently recognized species and, respectively, isolated from the cat and sheep. Both these strains shared low 16S rRNA gene sequence similarity, dDDH relatedness, and ANI values with their closest species C. upsaliensis CCUG 14913(T) and C. lanienae NCTC 13004(T), and against each other, which are below the cut-off values generally recognized for isolates of the same species. The genomic DNA G + C contents of type strains XJK22-1(T) and SYS25-1(T) were 34.99 mol% and 32.43 mol%, respectively. Electron microscopy showed that these cells were spiral shaped, with bipolar single flagella. Based on results from genotypic, phenotypic, phylogenetic, and phylogenomic analyses, these nine strains represent two novel species within the genus Campylobacter, for which the names Campylobacter felis sp. nov. (Type strain XJK22-1(T) = GDMCC 1.3684(T) = JCM 35847(T)) and Campylobacter ovis sp. nov. (Type strain SYS25-1(T) = GDMCC 1.3685(T)) are proposed. |
format | Online Article Text |
id | pubmed-10145079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101450792023-04-29 Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov Wang, Hairui Li, Ying Gu, Yixin Zhou, Guilan Chen, Xiaoli Zhang, Xin Shao, Zhujun Zhang, Jianzhong Zhang, Maojun Microorganisms Article Nine novel bacterial strains were isolated from the feces of cats and sheep in 2019 and 2020 in Beijing, China. Cells were 1–3 μm long and ≤0.5 μm wide, Gram-stain negative, microaerobic, motile, oxidase positive, and urease negative. Phylogenetic analyses based on 16S rRNA gene sequences indicated that these nine isolates belong to the genus Campylobacter but formed two robust clades that were clearly separate from the currently recognized species and, respectively, isolated from the cat and sheep. Both these strains shared low 16S rRNA gene sequence similarity, dDDH relatedness, and ANI values with their closest species C. upsaliensis CCUG 14913(T) and C. lanienae NCTC 13004(T), and against each other, which are below the cut-off values generally recognized for isolates of the same species. The genomic DNA G + C contents of type strains XJK22-1(T) and SYS25-1(T) were 34.99 mol% and 32.43 mol%, respectively. Electron microscopy showed that these cells were spiral shaped, with bipolar single flagella. Based on results from genotypic, phenotypic, phylogenetic, and phylogenomic analyses, these nine strains represent two novel species within the genus Campylobacter, for which the names Campylobacter felis sp. nov. (Type strain XJK22-1(T) = GDMCC 1.3684(T) = JCM 35847(T)) and Campylobacter ovis sp. nov. (Type strain SYS25-1(T) = GDMCC 1.3685(T)) are proposed. MDPI 2023-04-08 /pmc/articles/PMC10145079/ /pubmed/37110394 http://dx.doi.org/10.3390/microorganisms11040971 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Hairui Li, Ying Gu, Yixin Zhou, Guilan Chen, Xiaoli Zhang, Xin Shao, Zhujun Zhang, Jianzhong Zhang, Maojun Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov |
title | Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov |
title_full | Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov |
title_fullStr | Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov |
title_full_unstemmed | Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov |
title_short | Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov |
title_sort | isolation and genomic characteristics of cat-borne campylobacter felis sp. nov. and sheep-borne campylobacter ovis sp. nov |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145079/ https://www.ncbi.nlm.nih.gov/pubmed/37110394 http://dx.doi.org/10.3390/microorganisms11040971 |
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