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Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System

Rapid mutations within SARS-CoV-2 are driving immune escape, highlighting the need for in-depth and routine analysis of memory B cells (MBCs) to complement the important but limited information from neutralizing antibody (nAb) studies. In this study, we collected plasma samples and peripheral blood...

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Autores principales: Tsai, Dong-Yan, Wang, Chun-Hung, Schiro, Perry G., Chen, Nathan, Tseng, Ju-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145117/
https://www.ncbi.nlm.nih.gov/pubmed/37112647
http://dx.doi.org/10.3390/vaccines11040735
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author Tsai, Dong-Yan
Wang, Chun-Hung
Schiro, Perry G.
Chen, Nathan
Tseng, Ju-Yu
author_facet Tsai, Dong-Yan
Wang, Chun-Hung
Schiro, Perry G.
Chen, Nathan
Tseng, Ju-Yu
author_sort Tsai, Dong-Yan
collection PubMed
description Rapid mutations within SARS-CoV-2 are driving immune escape, highlighting the need for in-depth and routine analysis of memory B cells (MBCs) to complement the important but limited information from neutralizing antibody (nAb) studies. In this study, we collected plasma samples and peripheral blood mononuclear cells (PBMCs) from 35 subjects and studied the nAb titers and the number of antigen-specific memory B cells at designated time points before and after vaccination. We developed an assay to use the MiSelect R II System with a single-use microfluidic chip to directly detect the number of spike-receptor-binding domain (RBD)-specific MBCs in PBMCs. Our results show that the number of spike-RBD-specific MBCs detected by the MiSelect R II System is highly correlated with the level of nAbs secreted by stimulated PBMCs, even 6 months after vaccination when nAbs were generally not present in plasma. We also found antigen-specific cells recognizing Omicron spike-RBD were present in PBMCs from booster vaccination of subjects, but with a high variability in the number of B cells. The MiSelect R II System provided a direct, automated, and quantitative method to isolate and analyze subsets of rare cells for tracking cellular immunity in the context of a rapidly mutating virus.
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spelling pubmed-101451172023-04-29 Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System Tsai, Dong-Yan Wang, Chun-Hung Schiro, Perry G. Chen, Nathan Tseng, Ju-Yu Vaccines (Basel) Article Rapid mutations within SARS-CoV-2 are driving immune escape, highlighting the need for in-depth and routine analysis of memory B cells (MBCs) to complement the important but limited information from neutralizing antibody (nAb) studies. In this study, we collected plasma samples and peripheral blood mononuclear cells (PBMCs) from 35 subjects and studied the nAb titers and the number of antigen-specific memory B cells at designated time points before and after vaccination. We developed an assay to use the MiSelect R II System with a single-use microfluidic chip to directly detect the number of spike-receptor-binding domain (RBD)-specific MBCs in PBMCs. Our results show that the number of spike-RBD-specific MBCs detected by the MiSelect R II System is highly correlated with the level of nAbs secreted by stimulated PBMCs, even 6 months after vaccination when nAbs were generally not present in plasma. We also found antigen-specific cells recognizing Omicron spike-RBD were present in PBMCs from booster vaccination of subjects, but with a high variability in the number of B cells. The MiSelect R II System provided a direct, automated, and quantitative method to isolate and analyze subsets of rare cells for tracking cellular immunity in the context of a rapidly mutating virus. MDPI 2023-03-26 /pmc/articles/PMC10145117/ /pubmed/37112647 http://dx.doi.org/10.3390/vaccines11040735 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Dong-Yan
Wang, Chun-Hung
Schiro, Perry G.
Chen, Nathan
Tseng, Ju-Yu
Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System
title Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System
title_full Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System
title_fullStr Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System
title_full_unstemmed Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System
title_short Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System
title_sort tracking b cell memory to sars-cov-2 using rare cell analysis system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145117/
https://www.ncbi.nlm.nih.gov/pubmed/37112647
http://dx.doi.org/10.3390/vaccines11040735
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