Cargando…

Chitosan-Coated Solid Lipid Nanoparticles as an Efficient Avenue for Boosted Biological Activities of Aloe perryi: Antioxidant, Antibacterial, and Anticancer Potential

Aloe perryi (ALP) is an herb that has several biological activities such as antioxidant, antibacterial, and antitumor effects and is frequently used to treat a wide range of illnesses. The activity of many compounds is augmented by loading them in nanocarriers. In this study, ALP-loaded nanosystems...

Descripción completa

Detalles Bibliográficos
Autores principales: Aldayel, Tahany Saleh, M. Badran, Mohamed, H. Alomrani, Abdullah, AlFaris, Nora A., Z. Altamimi, Jozaa, S. Alqahtani, Ali, A. Nasr, Fahd, Ghaffar, Safina, Orfali, Raha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145204/
https://www.ncbi.nlm.nih.gov/pubmed/37110803
http://dx.doi.org/10.3390/molecules28083569
_version_ 1785034277014994944
author Aldayel, Tahany Saleh
M. Badran, Mohamed
H. Alomrani, Abdullah
AlFaris, Nora A.
Z. Altamimi, Jozaa
S. Alqahtani, Ali
A. Nasr, Fahd
Ghaffar, Safina
Orfali, Raha
author_facet Aldayel, Tahany Saleh
M. Badran, Mohamed
H. Alomrani, Abdullah
AlFaris, Nora A.
Z. Altamimi, Jozaa
S. Alqahtani, Ali
A. Nasr, Fahd
Ghaffar, Safina
Orfali, Raha
author_sort Aldayel, Tahany Saleh
collection PubMed
description Aloe perryi (ALP) is an herb that has several biological activities such as antioxidant, antibacterial, and antitumor effects and is frequently used to treat a wide range of illnesses. The activity of many compounds is augmented by loading them in nanocarriers. In this study, ALP-loaded nanosystems were developed to improve their biological activity. Among different nanocarriers, solid lipid nanoparticles (ALP-SLNs), chitosan nanoparticles (ALP-CSNPs), and CS-coated SLNs (C-ALP-SLNs) were explored. The particle size, polydispersity index (PDI), zeta potential, encapsulation efficiency, and release profile were evaluated. Scanning electron microscopy was used to see the nanoparticles’ morphology. Moreover, the possible biological properties of ALP were assessed and evaluated. ALP extract contained 187 mg GAE/g extract and 33 mg QE/g extract in terms of total phenolic and flavonoid content, respectively. The ALP-SLNs-F1 and ALP-SLNs-F2 showed particle sizes of 168.7 ± 3.1 and 138.4 ± 9.5 nm and the zeta potential values of −12.4 ± 0.6, and −15.8 ± 2.4 mV, respectively. However, C-ALP-SLNs-F1 and C-ALP-SLNs-F2 had particle sizes of 185.3 ± 5.5 and 173.6 ± 11.3 nm with zeta potential values of 11.3 ± 1.4 and 13.6 ± 1.1 mV, respectively. The particle size and zeta potential of ALP-CSNPs were 214.8 ± 6.6 nm and 27.8 ± 3.4 mV, respectively. All nanoparticles exhibited PDI < 0.3, indicating homogenous dispersions. The obtained formulations had EE% and DL% in the ranges of 65–82% and 2.8–5.2%, respectively. After 48 h, the in vitro ALP release rates from ALP-SLNs-F1, ALP-SLNs-F2, C-ALP-SLNs-F1, C-ALP-SLNs-F2, and ALP-CSNPs were 86%, 91%, 78%, 84%, and 74%, respectively. They were relatively stable with a minor particle size increase after one month of storage. C-ALP-SLNs-F2 exhibited the greatest antioxidant activity against DPPH radicals at 73.27%. C-ALP-SLNs-F2 demonstrated higher antibacterial activity based on MIC values of 25, 50, and 50 µg/mL for P. aeruginosa, S. aureus, and E. coli, respectively. In addition, C-ALP-SLNs-F2 showed potential anticancer activity against A549, LoVo, and MCF-7 cell lines with IC50 values of 11.42 ± 1.16, 16.97 ± 1.93, and 8.25 ± 0.44, respectively. The results indicate that C-ALP-SLNs-F2 may be promising nanocarriers for enhancing ALP-based medicines.
format Online
Article
Text
id pubmed-10145204
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101452042023-04-29 Chitosan-Coated Solid Lipid Nanoparticles as an Efficient Avenue for Boosted Biological Activities of Aloe perryi: Antioxidant, Antibacterial, and Anticancer Potential Aldayel, Tahany Saleh M. Badran, Mohamed H. Alomrani, Abdullah AlFaris, Nora A. Z. Altamimi, Jozaa S. Alqahtani, Ali A. Nasr, Fahd Ghaffar, Safina Orfali, Raha Molecules Article Aloe perryi (ALP) is an herb that has several biological activities such as antioxidant, antibacterial, and antitumor effects and is frequently used to treat a wide range of illnesses. The activity of many compounds is augmented by loading them in nanocarriers. In this study, ALP-loaded nanosystems were developed to improve their biological activity. Among different nanocarriers, solid lipid nanoparticles (ALP-SLNs), chitosan nanoparticles (ALP-CSNPs), and CS-coated SLNs (C-ALP-SLNs) were explored. The particle size, polydispersity index (PDI), zeta potential, encapsulation efficiency, and release profile were evaluated. Scanning electron microscopy was used to see the nanoparticles’ morphology. Moreover, the possible biological properties of ALP were assessed and evaluated. ALP extract contained 187 mg GAE/g extract and 33 mg QE/g extract in terms of total phenolic and flavonoid content, respectively. The ALP-SLNs-F1 and ALP-SLNs-F2 showed particle sizes of 168.7 ± 3.1 and 138.4 ± 9.5 nm and the zeta potential values of −12.4 ± 0.6, and −15.8 ± 2.4 mV, respectively. However, C-ALP-SLNs-F1 and C-ALP-SLNs-F2 had particle sizes of 185.3 ± 5.5 and 173.6 ± 11.3 nm with zeta potential values of 11.3 ± 1.4 and 13.6 ± 1.1 mV, respectively. The particle size and zeta potential of ALP-CSNPs were 214.8 ± 6.6 nm and 27.8 ± 3.4 mV, respectively. All nanoparticles exhibited PDI < 0.3, indicating homogenous dispersions. The obtained formulations had EE% and DL% in the ranges of 65–82% and 2.8–5.2%, respectively. After 48 h, the in vitro ALP release rates from ALP-SLNs-F1, ALP-SLNs-F2, C-ALP-SLNs-F1, C-ALP-SLNs-F2, and ALP-CSNPs were 86%, 91%, 78%, 84%, and 74%, respectively. They were relatively stable with a minor particle size increase after one month of storage. C-ALP-SLNs-F2 exhibited the greatest antioxidant activity against DPPH radicals at 73.27%. C-ALP-SLNs-F2 demonstrated higher antibacterial activity based on MIC values of 25, 50, and 50 µg/mL for P. aeruginosa, S. aureus, and E. coli, respectively. In addition, C-ALP-SLNs-F2 showed potential anticancer activity against A549, LoVo, and MCF-7 cell lines with IC50 values of 11.42 ± 1.16, 16.97 ± 1.93, and 8.25 ± 0.44, respectively. The results indicate that C-ALP-SLNs-F2 may be promising nanocarriers for enhancing ALP-based medicines. MDPI 2023-04-19 /pmc/articles/PMC10145204/ /pubmed/37110803 http://dx.doi.org/10.3390/molecules28083569 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aldayel, Tahany Saleh
M. Badran, Mohamed
H. Alomrani, Abdullah
AlFaris, Nora A.
Z. Altamimi, Jozaa
S. Alqahtani, Ali
A. Nasr, Fahd
Ghaffar, Safina
Orfali, Raha
Chitosan-Coated Solid Lipid Nanoparticles as an Efficient Avenue for Boosted Biological Activities of Aloe perryi: Antioxidant, Antibacterial, and Anticancer Potential
title Chitosan-Coated Solid Lipid Nanoparticles as an Efficient Avenue for Boosted Biological Activities of Aloe perryi: Antioxidant, Antibacterial, and Anticancer Potential
title_full Chitosan-Coated Solid Lipid Nanoparticles as an Efficient Avenue for Boosted Biological Activities of Aloe perryi: Antioxidant, Antibacterial, and Anticancer Potential
title_fullStr Chitosan-Coated Solid Lipid Nanoparticles as an Efficient Avenue for Boosted Biological Activities of Aloe perryi: Antioxidant, Antibacterial, and Anticancer Potential
title_full_unstemmed Chitosan-Coated Solid Lipid Nanoparticles as an Efficient Avenue for Boosted Biological Activities of Aloe perryi: Antioxidant, Antibacterial, and Anticancer Potential
title_short Chitosan-Coated Solid Lipid Nanoparticles as an Efficient Avenue for Boosted Biological Activities of Aloe perryi: Antioxidant, Antibacterial, and Anticancer Potential
title_sort chitosan-coated solid lipid nanoparticles as an efficient avenue for boosted biological activities of aloe perryi: antioxidant, antibacterial, and anticancer potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145204/
https://www.ncbi.nlm.nih.gov/pubmed/37110803
http://dx.doi.org/10.3390/molecules28083569
work_keys_str_mv AT aldayeltahanysaleh chitosancoatedsolidlipidnanoparticlesasanefficientavenueforboostedbiologicalactivitiesofaloeperryiantioxidantantibacterialandanticancerpotential
AT mbadranmohamed chitosancoatedsolidlipidnanoparticlesasanefficientavenueforboostedbiologicalactivitiesofaloeperryiantioxidantantibacterialandanticancerpotential
AT halomraniabdullah chitosancoatedsolidlipidnanoparticlesasanefficientavenueforboostedbiologicalactivitiesofaloeperryiantioxidantantibacterialandanticancerpotential
AT alfarisnoraa chitosancoatedsolidlipidnanoparticlesasanefficientavenueforboostedbiologicalactivitiesofaloeperryiantioxidantantibacterialandanticancerpotential
AT zaltamimijozaa chitosancoatedsolidlipidnanoparticlesasanefficientavenueforboostedbiologicalactivitiesofaloeperryiantioxidantantibacterialandanticancerpotential
AT salqahtaniali chitosancoatedsolidlipidnanoparticlesasanefficientavenueforboostedbiologicalactivitiesofaloeperryiantioxidantantibacterialandanticancerpotential
AT anasrfahd chitosancoatedsolidlipidnanoparticlesasanefficientavenueforboostedbiologicalactivitiesofaloeperryiantioxidantantibacterialandanticancerpotential
AT ghaffarsafina chitosancoatedsolidlipidnanoparticlesasanefficientavenueforboostedbiologicalactivitiesofaloeperryiantioxidantantibacterialandanticancerpotential
AT orfaliraha chitosancoatedsolidlipidnanoparticlesasanefficientavenueforboostedbiologicalactivitiesofaloeperryiantioxidantantibacterialandanticancerpotential