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Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients
Parkinson’s disease, one of the most common neurodegenerative diseases, may not only affect the motor system, but also the physiology of the gastrointestinal tract. Delayed gastric emptying, impaired motility and altered intestinal bacteria are well-established consequences of the disease, which can...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145225/ https://www.ncbi.nlm.nih.gov/pubmed/37111729 http://dx.doi.org/10.3390/pharmaceutics15041243 |
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author | de Waal, Tom Brouwers, Joachim Berben, Philippe Flanagan, Talia Tack, Jan Vandenberghe, Wim Vanuytsel, Tim Augustijns, Patrick |
author_facet | de Waal, Tom Brouwers, Joachim Berben, Philippe Flanagan, Talia Tack, Jan Vandenberghe, Wim Vanuytsel, Tim Augustijns, Patrick |
author_sort | de Waal, Tom |
collection | PubMed |
description | Parkinson’s disease, one of the most common neurodegenerative diseases, may not only affect the motor system, but also the physiology of the gastrointestinal tract. Delayed gastric emptying, impaired motility and altered intestinal bacteria are well-established consequences of the disease, which can have a pronounced effect on the absorption of orally administered drugs. In contrast, no studies have been performed into the composition of intestinal fluids. It is not unlikely that Parkinson’s disease also affects the composition of intestinal fluids, a critical factor in the in vitro and in silico simulation of drug dissolution, solubilization and absorption. In the current study, duodenal fluids were aspirated from Parkinson’s disease (PD) patients and age-matched healthy controls (healthy controls, HC) consecutively in fasted and fed conditions. The fluids were then characterized for pH, buffer capacity, osmolality, total protein, phospholipids, bile salts, cholesterol and lipids. In a fasted state, the intestinal fluid composition was highly similar in PD patients and healthy controls. In general, the same was true for fed-state fluids, apart from a slightly slower and less pronounced initial change in factors directly affected by the meal (i.e., buffer capacity, osmolality, total protein and lipids) in PD patients. The absence of a fast initial increase for these factors immediately after meal intake, as was observed in healthy controls, might result from slower gastric emptying in PD patients. Irrespective of the prandial state, a higher relative amount of secondary bile salts was observed in PD patients, potentially indicating altered intestinal bacterial metabolism. Overall, the data from this study indicate that only minor disease-specific adjustments in small intestinal fluid composition should be considered when simulating intestinal drug absorption in PD patients. |
format | Online Article Text |
id | pubmed-10145225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101452252023-04-29 Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients de Waal, Tom Brouwers, Joachim Berben, Philippe Flanagan, Talia Tack, Jan Vandenberghe, Wim Vanuytsel, Tim Augustijns, Patrick Pharmaceutics Article Parkinson’s disease, one of the most common neurodegenerative diseases, may not only affect the motor system, but also the physiology of the gastrointestinal tract. Delayed gastric emptying, impaired motility and altered intestinal bacteria are well-established consequences of the disease, which can have a pronounced effect on the absorption of orally administered drugs. In contrast, no studies have been performed into the composition of intestinal fluids. It is not unlikely that Parkinson’s disease also affects the composition of intestinal fluids, a critical factor in the in vitro and in silico simulation of drug dissolution, solubilization and absorption. In the current study, duodenal fluids were aspirated from Parkinson’s disease (PD) patients and age-matched healthy controls (healthy controls, HC) consecutively in fasted and fed conditions. The fluids were then characterized for pH, buffer capacity, osmolality, total protein, phospholipids, bile salts, cholesterol and lipids. In a fasted state, the intestinal fluid composition was highly similar in PD patients and healthy controls. In general, the same was true for fed-state fluids, apart from a slightly slower and less pronounced initial change in factors directly affected by the meal (i.e., buffer capacity, osmolality, total protein and lipids) in PD patients. The absence of a fast initial increase for these factors immediately after meal intake, as was observed in healthy controls, might result from slower gastric emptying in PD patients. Irrespective of the prandial state, a higher relative amount of secondary bile salts was observed in PD patients, potentially indicating altered intestinal bacterial metabolism. Overall, the data from this study indicate that only minor disease-specific adjustments in small intestinal fluid composition should be considered when simulating intestinal drug absorption in PD patients. MDPI 2023-04-14 /pmc/articles/PMC10145225/ /pubmed/37111729 http://dx.doi.org/10.3390/pharmaceutics15041243 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Waal, Tom Brouwers, Joachim Berben, Philippe Flanagan, Talia Tack, Jan Vandenberghe, Wim Vanuytsel, Tim Augustijns, Patrick Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients |
title | Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients |
title_full | Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients |
title_fullStr | Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients |
title_full_unstemmed | Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients |
title_short | Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients |
title_sort | characterization of aspirated duodenal fluids from parkinson’s disease patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145225/ https://www.ncbi.nlm.nih.gov/pubmed/37111729 http://dx.doi.org/10.3390/pharmaceutics15041243 |
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