Cargando…

Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients

Parkinson’s disease, one of the most common neurodegenerative diseases, may not only affect the motor system, but also the physiology of the gastrointestinal tract. Delayed gastric emptying, impaired motility and altered intestinal bacteria are well-established consequences of the disease, which can...

Descripción completa

Detalles Bibliográficos
Autores principales: de Waal, Tom, Brouwers, Joachim, Berben, Philippe, Flanagan, Talia, Tack, Jan, Vandenberghe, Wim, Vanuytsel, Tim, Augustijns, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145225/
https://www.ncbi.nlm.nih.gov/pubmed/37111729
http://dx.doi.org/10.3390/pharmaceutics15041243
_version_ 1785034282133094400
author de Waal, Tom
Brouwers, Joachim
Berben, Philippe
Flanagan, Talia
Tack, Jan
Vandenberghe, Wim
Vanuytsel, Tim
Augustijns, Patrick
author_facet de Waal, Tom
Brouwers, Joachim
Berben, Philippe
Flanagan, Talia
Tack, Jan
Vandenberghe, Wim
Vanuytsel, Tim
Augustijns, Patrick
author_sort de Waal, Tom
collection PubMed
description Parkinson’s disease, one of the most common neurodegenerative diseases, may not only affect the motor system, but also the physiology of the gastrointestinal tract. Delayed gastric emptying, impaired motility and altered intestinal bacteria are well-established consequences of the disease, which can have a pronounced effect on the absorption of orally administered drugs. In contrast, no studies have been performed into the composition of intestinal fluids. It is not unlikely that Parkinson’s disease also affects the composition of intestinal fluids, a critical factor in the in vitro and in silico simulation of drug dissolution, solubilization and absorption. In the current study, duodenal fluids were aspirated from Parkinson’s disease (PD) patients and age-matched healthy controls (healthy controls, HC) consecutively in fasted and fed conditions. The fluids were then characterized for pH, buffer capacity, osmolality, total protein, phospholipids, bile salts, cholesterol and lipids. In a fasted state, the intestinal fluid composition was highly similar in PD patients and healthy controls. In general, the same was true for fed-state fluids, apart from a slightly slower and less pronounced initial change in factors directly affected by the meal (i.e., buffer capacity, osmolality, total protein and lipids) in PD patients. The absence of a fast initial increase for these factors immediately after meal intake, as was observed in healthy controls, might result from slower gastric emptying in PD patients. Irrespective of the prandial state, a higher relative amount of secondary bile salts was observed in PD patients, potentially indicating altered intestinal bacterial metabolism. Overall, the data from this study indicate that only minor disease-specific adjustments in small intestinal fluid composition should be considered when simulating intestinal drug absorption in PD patients.
format Online
Article
Text
id pubmed-10145225
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-101452252023-04-29 Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients de Waal, Tom Brouwers, Joachim Berben, Philippe Flanagan, Talia Tack, Jan Vandenberghe, Wim Vanuytsel, Tim Augustijns, Patrick Pharmaceutics Article Parkinson’s disease, one of the most common neurodegenerative diseases, may not only affect the motor system, but also the physiology of the gastrointestinal tract. Delayed gastric emptying, impaired motility and altered intestinal bacteria are well-established consequences of the disease, which can have a pronounced effect on the absorption of orally administered drugs. In contrast, no studies have been performed into the composition of intestinal fluids. It is not unlikely that Parkinson’s disease also affects the composition of intestinal fluids, a critical factor in the in vitro and in silico simulation of drug dissolution, solubilization and absorption. In the current study, duodenal fluids were aspirated from Parkinson’s disease (PD) patients and age-matched healthy controls (healthy controls, HC) consecutively in fasted and fed conditions. The fluids were then characterized for pH, buffer capacity, osmolality, total protein, phospholipids, bile salts, cholesterol and lipids. In a fasted state, the intestinal fluid composition was highly similar in PD patients and healthy controls. In general, the same was true for fed-state fluids, apart from a slightly slower and less pronounced initial change in factors directly affected by the meal (i.e., buffer capacity, osmolality, total protein and lipids) in PD patients. The absence of a fast initial increase for these factors immediately after meal intake, as was observed in healthy controls, might result from slower gastric emptying in PD patients. Irrespective of the prandial state, a higher relative amount of secondary bile salts was observed in PD patients, potentially indicating altered intestinal bacterial metabolism. Overall, the data from this study indicate that only minor disease-specific adjustments in small intestinal fluid composition should be considered when simulating intestinal drug absorption in PD patients. MDPI 2023-04-14 /pmc/articles/PMC10145225/ /pubmed/37111729 http://dx.doi.org/10.3390/pharmaceutics15041243 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Waal, Tom
Brouwers, Joachim
Berben, Philippe
Flanagan, Talia
Tack, Jan
Vandenberghe, Wim
Vanuytsel, Tim
Augustijns, Patrick
Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients
title Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients
title_full Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients
title_fullStr Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients
title_full_unstemmed Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients
title_short Characterization of Aspirated Duodenal Fluids from Parkinson’s Disease Patients
title_sort characterization of aspirated duodenal fluids from parkinson’s disease patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145225/
https://www.ncbi.nlm.nih.gov/pubmed/37111729
http://dx.doi.org/10.3390/pharmaceutics15041243
work_keys_str_mv AT dewaaltom characterizationofaspiratedduodenalfluidsfromparkinsonsdiseasepatients
AT brouwersjoachim characterizationofaspiratedduodenalfluidsfromparkinsonsdiseasepatients
AT berbenphilippe characterizationofaspiratedduodenalfluidsfromparkinsonsdiseasepatients
AT flanagantalia characterizationofaspiratedduodenalfluidsfromparkinsonsdiseasepatients
AT tackjan characterizationofaspiratedduodenalfluidsfromparkinsonsdiseasepatients
AT vandenberghewim characterizationofaspiratedduodenalfluidsfromparkinsonsdiseasepatients
AT vanuytseltim characterizationofaspiratedduodenalfluidsfromparkinsonsdiseasepatients
AT augustijnspatrick characterizationofaspiratedduodenalfluidsfromparkinsonsdiseasepatients