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Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics

High-resolution mass spectrometry (HRMS) was coupled with ultra-high-performance liquid chromatography (UHPLC) to simultaneously quantify trehalose and trehalose 6-phosphate without derivatization or sample preparation. The use of full scan mode and exact mass analysis also makes it possible to carr...

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Autores principales: Tao, Ye, Rossez, Yannick, Bortolus, Clovis, Duma, Luminita, Dubar, Faustine, Merlier, Franck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145281/
https://www.ncbi.nlm.nih.gov/pubmed/37110679
http://dx.doi.org/10.3390/molecules28083443
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author Tao, Ye
Rossez, Yannick
Bortolus, Clovis
Duma, Luminita
Dubar, Faustine
Merlier, Franck
author_facet Tao, Ye
Rossez, Yannick
Bortolus, Clovis
Duma, Luminita
Dubar, Faustine
Merlier, Franck
author_sort Tao, Ye
collection PubMed
description High-resolution mass spectrometry (HRMS) was coupled with ultra-high-performance liquid chromatography (UHPLC) to simultaneously quantify trehalose and trehalose 6-phosphate without derivatization or sample preparation. The use of full scan mode and exact mass analysis also makes it possible to carry out metabolomic analyses as well as semi-quantification. In addition, the use of different clusters in negative mode makes it possible to compensate for deficiencies in linearity and inerrant saturation at time-of-flight detectors. The method has been approved and validated for different matrices, yeasts, and bacteria, and has shown differentiation between bacteria as a function of growth temperatures.
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spelling pubmed-101452812023-04-29 Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics Tao, Ye Rossez, Yannick Bortolus, Clovis Duma, Luminita Dubar, Faustine Merlier, Franck Molecules Article High-resolution mass spectrometry (HRMS) was coupled with ultra-high-performance liquid chromatography (UHPLC) to simultaneously quantify trehalose and trehalose 6-phosphate without derivatization or sample preparation. The use of full scan mode and exact mass analysis also makes it possible to carry out metabolomic analyses as well as semi-quantification. In addition, the use of different clusters in negative mode makes it possible to compensate for deficiencies in linearity and inerrant saturation at time-of-flight detectors. The method has been approved and validated for different matrices, yeasts, and bacteria, and has shown differentiation between bacteria as a function of growth temperatures. MDPI 2023-04-13 /pmc/articles/PMC10145281/ /pubmed/37110679 http://dx.doi.org/10.3390/molecules28083443 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tao, Ye
Rossez, Yannick
Bortolus, Clovis
Duma, Luminita
Dubar, Faustine
Merlier, Franck
Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics
title Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics
title_full Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics
title_fullStr Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics
title_full_unstemmed Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics
title_short Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics
title_sort simultaneous quantification of trehalose and trehalose 6-phosphate by hydrophilic interaction chromatography/electrospray accurate mass spectrometry with application in non-targeted metabolomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145281/
https://www.ncbi.nlm.nih.gov/pubmed/37110679
http://dx.doi.org/10.3390/molecules28083443
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