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Gene Expression of Inflammatory Cytokines in Major Organs by Extracorporeal Circulation

(1) Background: Extracorporeal circulation (ECC) is indispensable for cardiac surgery. Despite the fact that ECC causes non-physiological damage to blood components, its pathophysiology has not been fully elucidated. In our previous study, we constructed a rat ECC system and observed a systemic infl...

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Autores principales: Abe, Takuya, Hanawa, Haruo, Fujii, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145324/
https://www.ncbi.nlm.nih.gov/pubmed/37109150
http://dx.doi.org/10.3390/jcm12082813
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author Abe, Takuya
Hanawa, Haruo
Fujii, Yutaka
author_facet Abe, Takuya
Hanawa, Haruo
Fujii, Yutaka
author_sort Abe, Takuya
collection PubMed
description (1) Background: Extracorporeal circulation (ECC) is indispensable for cardiac surgery. Despite the fact that ECC causes non-physiological damage to blood components, its pathophysiology has not been fully elucidated. In our previous study, we constructed a rat ECC system and observed a systemic inflammatory response during and after blood tests assessing ECC, while the damage per organ localization caused by ECC was not examined. In this study, we used a rat model to assess the gene expression of inflammatory cytokines in major organs during ECC. (2) Methods: The ECC system consisted of a membranous oxygenator, tubing line, and a small roller pump. Rats were divided into a SHAM (which received surgical preparation only, without ECC) group and an ECC group. Proinflammatory cytokines were measured using real-time PCR in major organs after ECC to evaluate local inflammatory responses in the organs. (3) Results: Interleukin (IL)-6 levels were significantly elevated in the ECC group compared to the SHAM group, especially in the heart and lungs. (4) Conclusions: This study suggests that ECC promotes organ damage and the inflammatory response, but the degree of gene expression of proinflammatory cytokines varies from organ to organ, suggesting that it does not uniformly cause organ damage.
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spelling pubmed-101453242023-04-29 Gene Expression of Inflammatory Cytokines in Major Organs by Extracorporeal Circulation Abe, Takuya Hanawa, Haruo Fujii, Yutaka J Clin Med Brief Report (1) Background: Extracorporeal circulation (ECC) is indispensable for cardiac surgery. Despite the fact that ECC causes non-physiological damage to blood components, its pathophysiology has not been fully elucidated. In our previous study, we constructed a rat ECC system and observed a systemic inflammatory response during and after blood tests assessing ECC, while the damage per organ localization caused by ECC was not examined. In this study, we used a rat model to assess the gene expression of inflammatory cytokines in major organs during ECC. (2) Methods: The ECC system consisted of a membranous oxygenator, tubing line, and a small roller pump. Rats were divided into a SHAM (which received surgical preparation only, without ECC) group and an ECC group. Proinflammatory cytokines were measured using real-time PCR in major organs after ECC to evaluate local inflammatory responses in the organs. (3) Results: Interleukin (IL)-6 levels were significantly elevated in the ECC group compared to the SHAM group, especially in the heart and lungs. (4) Conclusions: This study suggests that ECC promotes organ damage and the inflammatory response, but the degree of gene expression of proinflammatory cytokines varies from organ to organ, suggesting that it does not uniformly cause organ damage. MDPI 2023-04-11 /pmc/articles/PMC10145324/ /pubmed/37109150 http://dx.doi.org/10.3390/jcm12082813 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Abe, Takuya
Hanawa, Haruo
Fujii, Yutaka
Gene Expression of Inflammatory Cytokines in Major Organs by Extracorporeal Circulation
title Gene Expression of Inflammatory Cytokines in Major Organs by Extracorporeal Circulation
title_full Gene Expression of Inflammatory Cytokines in Major Organs by Extracorporeal Circulation
title_fullStr Gene Expression of Inflammatory Cytokines in Major Organs by Extracorporeal Circulation
title_full_unstemmed Gene Expression of Inflammatory Cytokines in Major Organs by Extracorporeal Circulation
title_short Gene Expression of Inflammatory Cytokines in Major Organs by Extracorporeal Circulation
title_sort gene expression of inflammatory cytokines in major organs by extracorporeal circulation
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145324/
https://www.ncbi.nlm.nih.gov/pubmed/37109150
http://dx.doi.org/10.3390/jcm12082813
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