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Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia
A wide array of bacteria and fungi are known for their association with pests that impact the health of the olive tree. The latter presents the most economically important cultivation in Tunisia. The microbial diversity associated with olive orchards in Tunisia remains unknown and undetermined. This...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145363/ https://www.ncbi.nlm.nih.gov/pubmed/37110509 http://dx.doi.org/10.3390/microorganisms11041086 |
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author | Gharsallah, Houda Ksentini, Ines Frikha-Gargouri, Olfa Hadj Taieb, Karama Ben Gharsa, Haifa Schuster, Christina Chatti-kolsi, Amel Triki, Mohamed Ali Ksantini, Mohieddine Leclerque, Andreas |
author_facet | Gharsallah, Houda Ksentini, Ines Frikha-Gargouri, Olfa Hadj Taieb, Karama Ben Gharsa, Haifa Schuster, Christina Chatti-kolsi, Amel Triki, Mohamed Ali Ksantini, Mohieddine Leclerque, Andreas |
author_sort | Gharsallah, Houda |
collection | PubMed |
description | A wide array of bacteria and fungi are known for their association with pests that impact the health of the olive tree. The latter presents the most economically important cultivation in Tunisia. The microbial diversity associated with olive orchards in Tunisia remains unknown and undetermined. This study investigated microbial diversity to elucidate the microbial interactions that lead to olive disease, and the bio-prospects for potential microbial biocontrol agents associated with insect pests of economic relevance for olive cultivation in the Mediterranean area. Bacterial and fungal isolation was made from soil and olive tree pests. A total of 215 bacterial and fungal strains were randomly isolated from eight different biotopes situated in Sfax (Tunisia), with different management practices. 16S rRNA and ITS gene sequencing were used to identify the microbial community. The majority of the isolated bacteria, Staphylococcus, Bacillus, Alcaligenes, and Providencia, are typical of the olive ecosystem and the most common fungi are Penicillium, Aspergillus, and Cladosporium. The different olive orchards depicted distinct communities, and exhibited dissimilar amounts of bacteria and fungi with distinct ecological functions that could be considered as promising resources in biological control. |
format | Online Article Text |
id | pubmed-10145363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101453632023-04-29 Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia Gharsallah, Houda Ksentini, Ines Frikha-Gargouri, Olfa Hadj Taieb, Karama Ben Gharsa, Haifa Schuster, Christina Chatti-kolsi, Amel Triki, Mohamed Ali Ksantini, Mohieddine Leclerque, Andreas Microorganisms Article A wide array of bacteria and fungi are known for their association with pests that impact the health of the olive tree. The latter presents the most economically important cultivation in Tunisia. The microbial diversity associated with olive orchards in Tunisia remains unknown and undetermined. This study investigated microbial diversity to elucidate the microbial interactions that lead to olive disease, and the bio-prospects for potential microbial biocontrol agents associated with insect pests of economic relevance for olive cultivation in the Mediterranean area. Bacterial and fungal isolation was made from soil and olive tree pests. A total of 215 bacterial and fungal strains were randomly isolated from eight different biotopes situated in Sfax (Tunisia), with different management practices. 16S rRNA and ITS gene sequencing were used to identify the microbial community. The majority of the isolated bacteria, Staphylococcus, Bacillus, Alcaligenes, and Providencia, are typical of the olive ecosystem and the most common fungi are Penicillium, Aspergillus, and Cladosporium. The different olive orchards depicted distinct communities, and exhibited dissimilar amounts of bacteria and fungi with distinct ecological functions that could be considered as promising resources in biological control. MDPI 2023-04-21 /pmc/articles/PMC10145363/ /pubmed/37110509 http://dx.doi.org/10.3390/microorganisms11041086 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gharsallah, Houda Ksentini, Ines Frikha-Gargouri, Olfa Hadj Taieb, Karama Ben Gharsa, Haifa Schuster, Christina Chatti-kolsi, Amel Triki, Mohamed Ali Ksantini, Mohieddine Leclerque, Andreas Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia |
title | Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia |
title_full | Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia |
title_fullStr | Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia |
title_full_unstemmed | Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia |
title_short | Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia |
title_sort | exploring bacterial and fungal biodiversity in eight mediterranean olive orchards (olea europaea l.) in tunisia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145363/ https://www.ncbi.nlm.nih.gov/pubmed/37110509 http://dx.doi.org/10.3390/microorganisms11041086 |
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