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Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia

A wide array of bacteria and fungi are known for their association with pests that impact the health of the olive tree. The latter presents the most economically important cultivation in Tunisia. The microbial diversity associated with olive orchards in Tunisia remains unknown and undetermined. This...

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Autores principales: Gharsallah, Houda, Ksentini, Ines, Frikha-Gargouri, Olfa, Hadj Taieb, Karama, Ben Gharsa, Haifa, Schuster, Christina, Chatti-kolsi, Amel, Triki, Mohamed Ali, Ksantini, Mohieddine, Leclerque, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145363/
https://www.ncbi.nlm.nih.gov/pubmed/37110509
http://dx.doi.org/10.3390/microorganisms11041086
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author Gharsallah, Houda
Ksentini, Ines
Frikha-Gargouri, Olfa
Hadj Taieb, Karama
Ben Gharsa, Haifa
Schuster, Christina
Chatti-kolsi, Amel
Triki, Mohamed Ali
Ksantini, Mohieddine
Leclerque, Andreas
author_facet Gharsallah, Houda
Ksentini, Ines
Frikha-Gargouri, Olfa
Hadj Taieb, Karama
Ben Gharsa, Haifa
Schuster, Christina
Chatti-kolsi, Amel
Triki, Mohamed Ali
Ksantini, Mohieddine
Leclerque, Andreas
author_sort Gharsallah, Houda
collection PubMed
description A wide array of bacteria and fungi are known for their association with pests that impact the health of the olive tree. The latter presents the most economically important cultivation in Tunisia. The microbial diversity associated with olive orchards in Tunisia remains unknown and undetermined. This study investigated microbial diversity to elucidate the microbial interactions that lead to olive disease, and the bio-prospects for potential microbial biocontrol agents associated with insect pests of economic relevance for olive cultivation in the Mediterranean area. Bacterial and fungal isolation was made from soil and olive tree pests. A total of 215 bacterial and fungal strains were randomly isolated from eight different biotopes situated in Sfax (Tunisia), with different management practices. 16S rRNA and ITS gene sequencing were used to identify the microbial community. The majority of the isolated bacteria, Staphylococcus, Bacillus, Alcaligenes, and Providencia, are typical of the olive ecosystem and the most common fungi are Penicillium, Aspergillus, and Cladosporium. The different olive orchards depicted distinct communities, and exhibited dissimilar amounts of bacteria and fungi with distinct ecological functions that could be considered as promising resources in biological control.
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spelling pubmed-101453632023-04-29 Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia Gharsallah, Houda Ksentini, Ines Frikha-Gargouri, Olfa Hadj Taieb, Karama Ben Gharsa, Haifa Schuster, Christina Chatti-kolsi, Amel Triki, Mohamed Ali Ksantini, Mohieddine Leclerque, Andreas Microorganisms Article A wide array of bacteria and fungi are known for their association with pests that impact the health of the olive tree. The latter presents the most economically important cultivation in Tunisia. The microbial diversity associated with olive orchards in Tunisia remains unknown and undetermined. This study investigated microbial diversity to elucidate the microbial interactions that lead to olive disease, and the bio-prospects for potential microbial biocontrol agents associated with insect pests of economic relevance for olive cultivation in the Mediterranean area. Bacterial and fungal isolation was made from soil and olive tree pests. A total of 215 bacterial and fungal strains were randomly isolated from eight different biotopes situated in Sfax (Tunisia), with different management practices. 16S rRNA and ITS gene sequencing were used to identify the microbial community. The majority of the isolated bacteria, Staphylococcus, Bacillus, Alcaligenes, and Providencia, are typical of the olive ecosystem and the most common fungi are Penicillium, Aspergillus, and Cladosporium. The different olive orchards depicted distinct communities, and exhibited dissimilar amounts of bacteria and fungi with distinct ecological functions that could be considered as promising resources in biological control. MDPI 2023-04-21 /pmc/articles/PMC10145363/ /pubmed/37110509 http://dx.doi.org/10.3390/microorganisms11041086 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gharsallah, Houda
Ksentini, Ines
Frikha-Gargouri, Olfa
Hadj Taieb, Karama
Ben Gharsa, Haifa
Schuster, Christina
Chatti-kolsi, Amel
Triki, Mohamed Ali
Ksantini, Mohieddine
Leclerque, Andreas
Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia
title Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia
title_full Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia
title_fullStr Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia
title_full_unstemmed Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia
title_short Exploring Bacterial and Fungal Biodiversity in Eight Mediterranean Olive Orchards (Olea europaea L.) in Tunisia
title_sort exploring bacterial and fungal biodiversity in eight mediterranean olive orchards (olea europaea l.) in tunisia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145363/
https://www.ncbi.nlm.nih.gov/pubmed/37110509
http://dx.doi.org/10.3390/microorganisms11041086
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