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Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov

For more than a century, lichens have been used as an example of dual-partner symbiosis. Recently, this has been challenged by the discovery of various basidiomycetous yeasts that coexist in multiple lichen species, among which Cladonia lichens from Europe and the United States were discovered to be...

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Autores principales: Nguyen, Ngoc-Hung, Nguyen, Phuong-Thao, Otake, Hitomi, Nagata, Ayana, Hirano, Nobuharu, Imanishi-Shimizu, Yumi, Shimizu, Kiminori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145395/
https://www.ncbi.nlm.nih.gov/pubmed/37108927
http://dx.doi.org/10.3390/jof9040473
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author Nguyen, Ngoc-Hung
Nguyen, Phuong-Thao
Otake, Hitomi
Nagata, Ayana
Hirano, Nobuharu
Imanishi-Shimizu, Yumi
Shimizu, Kiminori
author_facet Nguyen, Ngoc-Hung
Nguyen, Phuong-Thao
Otake, Hitomi
Nagata, Ayana
Hirano, Nobuharu
Imanishi-Shimizu, Yumi
Shimizu, Kiminori
author_sort Nguyen, Ngoc-Hung
collection PubMed
description For more than a century, lichens have been used as an example of dual-partner symbiosis. Recently, this has been challenged by the discovery of various basidiomycetous yeasts that coexist in multiple lichen species, among which Cladonia lichens from Europe and the United States were discovered to be highly specifically associated with the basidiomycetous yeast of the family Microsporomycetaceae. To verify this highly specific relationship, we investigated the diversity of basidiomycetous yeasts associated with Cladonia rei, a widely distributed lichen in Japan, by applying two approaches: yeast isolation from the lichen thalli and meta-barcoding analysis. We obtained 42 cultures of Cystobasidiomycetous yeast which were grouped into six lineages within the family Microsporomycetaceae. Unexpectedly, although the cystobasidiomycetes-specific primer was used, not only the cystobasidiomycetous yeasts but species from other classes were also detected via the meta-barcoding dataset; in particular, pucciniomycetous yeasts were found at a high frequency in some samples. Further, Halobasidium xiangyangense, which was detected in every sample with high abundance, is highly likely a generalist epiphytic fungus that has the ability to associate with C. rei. In the pucciniomycetous group, most of the detected species belong to the scale insect-associated yeast Septobasidium genus. In conclusion, even though Microsporomyces species are not the only yeast group associated with Cladonia lichen, our study demonstrated that the thalli of Cladonia rei lichen could be a suitable habit for them.
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spelling pubmed-101453952023-04-29 Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov Nguyen, Ngoc-Hung Nguyen, Phuong-Thao Otake, Hitomi Nagata, Ayana Hirano, Nobuharu Imanishi-Shimizu, Yumi Shimizu, Kiminori J Fungi (Basel) Article For more than a century, lichens have been used as an example of dual-partner symbiosis. Recently, this has been challenged by the discovery of various basidiomycetous yeasts that coexist in multiple lichen species, among which Cladonia lichens from Europe and the United States were discovered to be highly specifically associated with the basidiomycetous yeast of the family Microsporomycetaceae. To verify this highly specific relationship, we investigated the diversity of basidiomycetous yeasts associated with Cladonia rei, a widely distributed lichen in Japan, by applying two approaches: yeast isolation from the lichen thalli and meta-barcoding analysis. We obtained 42 cultures of Cystobasidiomycetous yeast which were grouped into six lineages within the family Microsporomycetaceae. Unexpectedly, although the cystobasidiomycetes-specific primer was used, not only the cystobasidiomycetous yeasts but species from other classes were also detected via the meta-barcoding dataset; in particular, pucciniomycetous yeasts were found at a high frequency in some samples. Further, Halobasidium xiangyangense, which was detected in every sample with high abundance, is highly likely a generalist epiphytic fungus that has the ability to associate with C. rei. In the pucciniomycetous group, most of the detected species belong to the scale insect-associated yeast Septobasidium genus. In conclusion, even though Microsporomyces species are not the only yeast group associated with Cladonia lichen, our study demonstrated that the thalli of Cladonia rei lichen could be a suitable habit for them. MDPI 2023-04-14 /pmc/articles/PMC10145395/ /pubmed/37108927 http://dx.doi.org/10.3390/jof9040473 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nguyen, Ngoc-Hung
Nguyen, Phuong-Thao
Otake, Hitomi
Nagata, Ayana
Hirano, Nobuharu
Imanishi-Shimizu, Yumi
Shimizu, Kiminori
Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov
title Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov
title_full Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov
title_fullStr Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov
title_full_unstemmed Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov
title_short Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov
title_sort biodiversity of basidiomycetous yeasts associated with cladonia rei lichen in japan, with a description of microsporomyces cladoniophilus sp. nov
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145395/
https://www.ncbi.nlm.nih.gov/pubmed/37108927
http://dx.doi.org/10.3390/jof9040473
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