Cargando…
Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov
For more than a century, lichens have been used as an example of dual-partner symbiosis. Recently, this has been challenged by the discovery of various basidiomycetous yeasts that coexist in multiple lichen species, among which Cladonia lichens from Europe and the United States were discovered to be...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145395/ https://www.ncbi.nlm.nih.gov/pubmed/37108927 http://dx.doi.org/10.3390/jof9040473 |
_version_ | 1785034323211059200 |
---|---|
author | Nguyen, Ngoc-Hung Nguyen, Phuong-Thao Otake, Hitomi Nagata, Ayana Hirano, Nobuharu Imanishi-Shimizu, Yumi Shimizu, Kiminori |
author_facet | Nguyen, Ngoc-Hung Nguyen, Phuong-Thao Otake, Hitomi Nagata, Ayana Hirano, Nobuharu Imanishi-Shimizu, Yumi Shimizu, Kiminori |
author_sort | Nguyen, Ngoc-Hung |
collection | PubMed |
description | For more than a century, lichens have been used as an example of dual-partner symbiosis. Recently, this has been challenged by the discovery of various basidiomycetous yeasts that coexist in multiple lichen species, among which Cladonia lichens from Europe and the United States were discovered to be highly specifically associated with the basidiomycetous yeast of the family Microsporomycetaceae. To verify this highly specific relationship, we investigated the diversity of basidiomycetous yeasts associated with Cladonia rei, a widely distributed lichen in Japan, by applying two approaches: yeast isolation from the lichen thalli and meta-barcoding analysis. We obtained 42 cultures of Cystobasidiomycetous yeast which were grouped into six lineages within the family Microsporomycetaceae. Unexpectedly, although the cystobasidiomycetes-specific primer was used, not only the cystobasidiomycetous yeasts but species from other classes were also detected via the meta-barcoding dataset; in particular, pucciniomycetous yeasts were found at a high frequency in some samples. Further, Halobasidium xiangyangense, which was detected in every sample with high abundance, is highly likely a generalist epiphytic fungus that has the ability to associate with C. rei. In the pucciniomycetous group, most of the detected species belong to the scale insect-associated yeast Septobasidium genus. In conclusion, even though Microsporomyces species are not the only yeast group associated with Cladonia lichen, our study demonstrated that the thalli of Cladonia rei lichen could be a suitable habit for them. |
format | Online Article Text |
id | pubmed-10145395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101453952023-04-29 Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov Nguyen, Ngoc-Hung Nguyen, Phuong-Thao Otake, Hitomi Nagata, Ayana Hirano, Nobuharu Imanishi-Shimizu, Yumi Shimizu, Kiminori J Fungi (Basel) Article For more than a century, lichens have been used as an example of dual-partner symbiosis. Recently, this has been challenged by the discovery of various basidiomycetous yeasts that coexist in multiple lichen species, among which Cladonia lichens from Europe and the United States were discovered to be highly specifically associated with the basidiomycetous yeast of the family Microsporomycetaceae. To verify this highly specific relationship, we investigated the diversity of basidiomycetous yeasts associated with Cladonia rei, a widely distributed lichen in Japan, by applying two approaches: yeast isolation from the lichen thalli and meta-barcoding analysis. We obtained 42 cultures of Cystobasidiomycetous yeast which were grouped into six lineages within the family Microsporomycetaceae. Unexpectedly, although the cystobasidiomycetes-specific primer was used, not only the cystobasidiomycetous yeasts but species from other classes were also detected via the meta-barcoding dataset; in particular, pucciniomycetous yeasts were found at a high frequency in some samples. Further, Halobasidium xiangyangense, which was detected in every sample with high abundance, is highly likely a generalist epiphytic fungus that has the ability to associate with C. rei. In the pucciniomycetous group, most of the detected species belong to the scale insect-associated yeast Septobasidium genus. In conclusion, even though Microsporomyces species are not the only yeast group associated with Cladonia lichen, our study demonstrated that the thalli of Cladonia rei lichen could be a suitable habit for them. MDPI 2023-04-14 /pmc/articles/PMC10145395/ /pubmed/37108927 http://dx.doi.org/10.3390/jof9040473 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nguyen, Ngoc-Hung Nguyen, Phuong-Thao Otake, Hitomi Nagata, Ayana Hirano, Nobuharu Imanishi-Shimizu, Yumi Shimizu, Kiminori Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov |
title | Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov |
title_full | Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov |
title_fullStr | Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov |
title_full_unstemmed | Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov |
title_short | Biodiversity of Basidiomycetous Yeasts Associated with Cladonia rei Lichen in Japan, with a Description of Microsporomyces cladoniophilus sp. nov |
title_sort | biodiversity of basidiomycetous yeasts associated with cladonia rei lichen in japan, with a description of microsporomyces cladoniophilus sp. nov |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145395/ https://www.ncbi.nlm.nih.gov/pubmed/37108927 http://dx.doi.org/10.3390/jof9040473 |
work_keys_str_mv | AT nguyenngochung biodiversityofbasidiomycetousyeastsassociatedwithcladoniareilicheninjapanwithadescriptionofmicrosporomycescladoniophilusspnov AT nguyenphuongthao biodiversityofbasidiomycetousyeastsassociatedwithcladoniareilicheninjapanwithadescriptionofmicrosporomycescladoniophilusspnov AT otakehitomi biodiversityofbasidiomycetousyeastsassociatedwithcladoniareilicheninjapanwithadescriptionofmicrosporomycescladoniophilusspnov AT nagataayana biodiversityofbasidiomycetousyeastsassociatedwithcladoniareilicheninjapanwithadescriptionofmicrosporomycescladoniophilusspnov AT hiranonobuharu biodiversityofbasidiomycetousyeastsassociatedwithcladoniareilicheninjapanwithadescriptionofmicrosporomycescladoniophilusspnov AT imanishishimizuyumi biodiversityofbasidiomycetousyeastsassociatedwithcladoniareilicheninjapanwithadescriptionofmicrosporomycescladoniophilusspnov AT shimizukiminori biodiversityofbasidiomycetousyeastsassociatedwithcladoniareilicheninjapanwithadescriptionofmicrosporomycescladoniophilusspnov |