Cargando…
Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of Schistosoma japonicum and Schistosomiasis Japonica
The prevalence and infectious intensity of schistosomiasis japonica has decreased significantly in China in the past few decades. However, more accurate and sensitive diagnostic methods are urgently required for the further control, surveillance, and final elimination of the disease. In this study,...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145555/ https://www.ncbi.nlm.nih.gov/pubmed/37110482 http://dx.doi.org/10.3390/microorganisms11041059 |
_version_ | 1785034361767198720 |
---|---|
author | Hong, Yang Guo, Qinghong Zhou, Xue Tang, Liying Chen, Cheng Shang, Zheng Zhou, Kerou Zhang, Zhizhong Liu, Jinming Lin, Jiaojiao Xu, Bin Chen, Jun-Hu Fu, Zhiqiang Hu, Wei |
author_facet | Hong, Yang Guo, Qinghong Zhou, Xue Tang, Liying Chen, Cheng Shang, Zheng Zhou, Kerou Zhang, Zhizhong Liu, Jinming Lin, Jiaojiao Xu, Bin Chen, Jun-Hu Fu, Zhiqiang Hu, Wei |
author_sort | Hong, Yang |
collection | PubMed |
description | The prevalence and infectious intensity of schistosomiasis japonica has decreased significantly in China in the past few decades. However, more accurate and sensitive diagnostic methods are urgently required for the further control, surveillance, and final elimination of the disease. In this study, we assessed the diagnostic efficacy of a real-time fluorescence quantitative PCR (qPCR) method and recombinase polymerase amplification (RPA) combined with a lateral-flow dipstick (LFD) assay for detecting early infections of Schistosoma japonicum and different infection intensities. The sensitivity of the qPCR at 40 days post-infection (dpi) was 100% (8/8) in mice infected with 40 cercariae, which was higher than in mice infected with 10 cercariae (90%, 9/10) or five cercariae (77.8%, 7/9). The results of the RPA–LFD assays were similar, with sensitivities of 55.6% (5/9), 80% (8/10), and 100% (8/8) in mice infected with 5, 10, and 40 cercariae, respectively. In goats, both the qPCR and RPA–LFD assays showed 100% (8/8) sensitivity at 56 dpi. In the early detection of S. japonicum infection in mice and goats with qPCR, the first peak in positivity appeared at 3–4 dpi, when the positivity rate exceeded 40%, even in the low infection, intensity mice. In the RPA–LFD assays, positive results first peaked at 4–5 dpi in the mice, and the positivity rate was 37.5% in the goats at 1 dpi. In conclusion, neither of the molecular methods produced exceptional results for the early diagnosis of S. japonicum infection. However, they were useful methods for the regular diagnosis of schistosomiasis in mice and goats. |
format | Online Article Text |
id | pubmed-10145555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101455552023-04-29 Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of Schistosoma japonicum and Schistosomiasis Japonica Hong, Yang Guo, Qinghong Zhou, Xue Tang, Liying Chen, Cheng Shang, Zheng Zhou, Kerou Zhang, Zhizhong Liu, Jinming Lin, Jiaojiao Xu, Bin Chen, Jun-Hu Fu, Zhiqiang Hu, Wei Microorganisms Article The prevalence and infectious intensity of schistosomiasis japonica has decreased significantly in China in the past few decades. However, more accurate and sensitive diagnostic methods are urgently required for the further control, surveillance, and final elimination of the disease. In this study, we assessed the diagnostic efficacy of a real-time fluorescence quantitative PCR (qPCR) method and recombinase polymerase amplification (RPA) combined with a lateral-flow dipstick (LFD) assay for detecting early infections of Schistosoma japonicum and different infection intensities. The sensitivity of the qPCR at 40 days post-infection (dpi) was 100% (8/8) in mice infected with 40 cercariae, which was higher than in mice infected with 10 cercariae (90%, 9/10) or five cercariae (77.8%, 7/9). The results of the RPA–LFD assays were similar, with sensitivities of 55.6% (5/9), 80% (8/10), and 100% (8/8) in mice infected with 5, 10, and 40 cercariae, respectively. In goats, both the qPCR and RPA–LFD assays showed 100% (8/8) sensitivity at 56 dpi. In the early detection of S. japonicum infection in mice and goats with qPCR, the first peak in positivity appeared at 3–4 dpi, when the positivity rate exceeded 40%, even in the low infection, intensity mice. In the RPA–LFD assays, positive results first peaked at 4–5 dpi in the mice, and the positivity rate was 37.5% in the goats at 1 dpi. In conclusion, neither of the molecular methods produced exceptional results for the early diagnosis of S. japonicum infection. However, they were useful methods for the regular diagnosis of schistosomiasis in mice and goats. MDPI 2023-04-18 /pmc/articles/PMC10145555/ /pubmed/37110482 http://dx.doi.org/10.3390/microorganisms11041059 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hong, Yang Guo, Qinghong Zhou, Xue Tang, Liying Chen, Cheng Shang, Zheng Zhou, Kerou Zhang, Zhizhong Liu, Jinming Lin, Jiaojiao Xu, Bin Chen, Jun-Hu Fu, Zhiqiang Hu, Wei Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of Schistosoma japonicum and Schistosomiasis Japonica |
title | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of Schistosoma japonicum and Schistosomiasis Japonica |
title_full | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of Schistosoma japonicum and Schistosomiasis Japonica |
title_fullStr | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of Schistosoma japonicum and Schistosomiasis Japonica |
title_full_unstemmed | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of Schistosoma japonicum and Schistosomiasis Japonica |
title_short | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of Schistosoma japonicum and Schistosomiasis Japonica |
title_sort | two molecular plasma-based diagnostic methods to evaluate early infection of schistosoma japonicum and schistosomiasis japonica |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145555/ https://www.ncbi.nlm.nih.gov/pubmed/37110482 http://dx.doi.org/10.3390/microorganisms11041059 |
work_keys_str_mv | AT hongyang twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT guoqinghong twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT zhouxue twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT tangliying twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT chencheng twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT shangzheng twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT zhoukerou twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT zhangzhizhong twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT liujinming twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT linjiaojiao twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT xubin twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT chenjunhu twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT fuzhiqiang twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica AT huwei twomolecularplasmabaseddiagnosticmethodstoevaluateearlyinfectionofschistosomajaponicumandschistosomiasisjaponica |