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Snapshot of Phenotypic and Molecular Virulence and Resistance Profiles in Multidrug-Resistant Strains Isolated in a Tertiary Hospital in Romania

A current major healthcare problem is represented by antibiotic resistance, mainly due to multidrug resistant (MDR) Gram negative bacilli (GNB), because of their extended spread both in hospital facilities and in the community’s environment. The aim of this study was to investigate the virulence tra...

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Autores principales: Truşcă, Bianca Simona, Gheorghe-Barbu, Irina, Manea, Marina, Ianculescu, Elvira, Barbu, Ilda Czobor, Măruțescu, Luminița Gabriela, Dițu, Lia-Mara, Chifiriuc, Mariana-Carmen, Lazăr, Veronica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145626/
https://www.ncbi.nlm.nih.gov/pubmed/37111495
http://dx.doi.org/10.3390/pathogens12040609
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author Truşcă, Bianca Simona
Gheorghe-Barbu, Irina
Manea, Marina
Ianculescu, Elvira
Barbu, Ilda Czobor
Măruțescu, Luminița Gabriela
Dițu, Lia-Mara
Chifiriuc, Mariana-Carmen
Lazăr, Veronica
author_facet Truşcă, Bianca Simona
Gheorghe-Barbu, Irina
Manea, Marina
Ianculescu, Elvira
Barbu, Ilda Czobor
Măruțescu, Luminița Gabriela
Dițu, Lia-Mara
Chifiriuc, Mariana-Carmen
Lazăr, Veronica
author_sort Truşcă, Bianca Simona
collection PubMed
description A current major healthcare problem is represented by antibiotic resistance, mainly due to multidrug resistant (MDR) Gram negative bacilli (GNB), because of their extended spread both in hospital facilities and in the community’s environment. The aim of this study was to investigate the virulence traits of Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa MDR, XDR, and PDR strains isolated from various hospitalized patients. These GNB strains were investigated for the presence of soluble virulence factors (VF), such as hemolysins, lecithinase, amylase, lipase, caseinase, gelatinase, and esculin hydrolysis, as well as for the presence of virulence genes encoding for VF involved in adherence (TC, fimH, and fimA), biofilm formation (algD, ecpRAB, mrkA, mrkD, ompA, and epsA), tissue destruction (plcH and plcN), and in toxin production (cnfI, hlyA, hlyD, and exo complex). All P. aeruginosa strains produced hemolysins; 90% produced lecithinase; and 80% harbored algD, plcH, and plcN genes. The esculin hydrolysis was detected in 96.1% of the K. pneumoniae strains, whereas 86% of them were positive for the mrkA gene. All of the A. baumannii strains produced lecithinase and 80% presented the ompA gene. A significant association was found between the number of VF and the XDR strains, regardless of the isolation sources. This study opens new research perspectives related to bacterial fitness and pathogenicity, and it provides new insights regarding the connection between biofilm formation, other virulence factors, and antibiotic resistance.
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spelling pubmed-101456262023-04-29 Snapshot of Phenotypic and Molecular Virulence and Resistance Profiles in Multidrug-Resistant Strains Isolated in a Tertiary Hospital in Romania Truşcă, Bianca Simona Gheorghe-Barbu, Irina Manea, Marina Ianculescu, Elvira Barbu, Ilda Czobor Măruțescu, Luminița Gabriela Dițu, Lia-Mara Chifiriuc, Mariana-Carmen Lazăr, Veronica Pathogens Article A current major healthcare problem is represented by antibiotic resistance, mainly due to multidrug resistant (MDR) Gram negative bacilli (GNB), because of their extended spread both in hospital facilities and in the community’s environment. The aim of this study was to investigate the virulence traits of Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa MDR, XDR, and PDR strains isolated from various hospitalized patients. These GNB strains were investigated for the presence of soluble virulence factors (VF), such as hemolysins, lecithinase, amylase, lipase, caseinase, gelatinase, and esculin hydrolysis, as well as for the presence of virulence genes encoding for VF involved in adherence (TC, fimH, and fimA), biofilm formation (algD, ecpRAB, mrkA, mrkD, ompA, and epsA), tissue destruction (plcH and plcN), and in toxin production (cnfI, hlyA, hlyD, and exo complex). All P. aeruginosa strains produced hemolysins; 90% produced lecithinase; and 80% harbored algD, plcH, and plcN genes. The esculin hydrolysis was detected in 96.1% of the K. pneumoniae strains, whereas 86% of them were positive for the mrkA gene. All of the A. baumannii strains produced lecithinase and 80% presented the ompA gene. A significant association was found between the number of VF and the XDR strains, regardless of the isolation sources. This study opens new research perspectives related to bacterial fitness and pathogenicity, and it provides new insights regarding the connection between biofilm formation, other virulence factors, and antibiotic resistance. MDPI 2023-04-17 /pmc/articles/PMC10145626/ /pubmed/37111495 http://dx.doi.org/10.3390/pathogens12040609 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Truşcă, Bianca Simona
Gheorghe-Barbu, Irina
Manea, Marina
Ianculescu, Elvira
Barbu, Ilda Czobor
Măruțescu, Luminița Gabriela
Dițu, Lia-Mara
Chifiriuc, Mariana-Carmen
Lazăr, Veronica
Snapshot of Phenotypic and Molecular Virulence and Resistance Profiles in Multidrug-Resistant Strains Isolated in a Tertiary Hospital in Romania
title Snapshot of Phenotypic and Molecular Virulence and Resistance Profiles in Multidrug-Resistant Strains Isolated in a Tertiary Hospital in Romania
title_full Snapshot of Phenotypic and Molecular Virulence and Resistance Profiles in Multidrug-Resistant Strains Isolated in a Tertiary Hospital in Romania
title_fullStr Snapshot of Phenotypic and Molecular Virulence and Resistance Profiles in Multidrug-Resistant Strains Isolated in a Tertiary Hospital in Romania
title_full_unstemmed Snapshot of Phenotypic and Molecular Virulence and Resistance Profiles in Multidrug-Resistant Strains Isolated in a Tertiary Hospital in Romania
title_short Snapshot of Phenotypic and Molecular Virulence and Resistance Profiles in Multidrug-Resistant Strains Isolated in a Tertiary Hospital in Romania
title_sort snapshot of phenotypic and molecular virulence and resistance profiles in multidrug-resistant strains isolated in a tertiary hospital in romania
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145626/
https://www.ncbi.nlm.nih.gov/pubmed/37111495
http://dx.doi.org/10.3390/pathogens12040609
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