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Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence
African swine fever (ASF) is a highly lethal hemorrhagic viral disease that causes extensive economic and animal welfare losses in the Eurasian pig (Sus scrofa) population. To date, no effective and safe vaccines have been marketed against ASF. A starting point for vaccine development is using natur...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145817/ https://www.ncbi.nlm.nih.gov/pubmed/37112759 http://dx.doi.org/10.3390/vaccines11040846 |
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author | Tamás, Vivien Righi, Cecilia Mészáros, István D’Errico, Federica Olasz, Ferenc Casciari, Cristina Zádori, Zoltán Magyar, Tibor Petrini, Stefano Feliziani, Francesco |
author_facet | Tamás, Vivien Righi, Cecilia Mészáros, István D’Errico, Federica Olasz, Ferenc Casciari, Cristina Zádori, Zoltán Magyar, Tibor Petrini, Stefano Feliziani, Francesco |
author_sort | Tamás, Vivien |
collection | PubMed |
description | African swine fever (ASF) is a highly lethal hemorrhagic viral disease that causes extensive economic and animal welfare losses in the Eurasian pig (Sus scrofa) population. To date, no effective and safe vaccines have been marketed against ASF. A starting point for vaccine development is using naturally occurring attenuated strains as a vaccine base. Here, we aimed to remove the multigene family (MGF) 110 gene of unknown function from the Lv17/WB/Rie1 genome to improve the usability of the virus as a live-attenuated vaccine, reducing unwanted side effects. The MGF 110-11L gene was deleted using the CRISPR/Cas9 method, and the safety and efficacy of the virus were tested in pigs after isolation. The vaccine candidates administered at high doses showed reduced pathogenicity compared to the parental strain and induced immunity in vaccinated animals, although several mild clinical signs were observed. Although Lv17/WB/Rie1/d110-11L cannot be used as a vaccine in its current form, it was encouraging that the undesirable side effects of Lv17/WB/Rie1 at high doses can be reduced by additional mutations without a significant reduction in its protective capacity. |
format | Online Article Text |
id | pubmed-10145817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101458172023-04-29 Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence Tamás, Vivien Righi, Cecilia Mészáros, István D’Errico, Federica Olasz, Ferenc Casciari, Cristina Zádori, Zoltán Magyar, Tibor Petrini, Stefano Feliziani, Francesco Vaccines (Basel) Article African swine fever (ASF) is a highly lethal hemorrhagic viral disease that causes extensive economic and animal welfare losses in the Eurasian pig (Sus scrofa) population. To date, no effective and safe vaccines have been marketed against ASF. A starting point for vaccine development is using naturally occurring attenuated strains as a vaccine base. Here, we aimed to remove the multigene family (MGF) 110 gene of unknown function from the Lv17/WB/Rie1 genome to improve the usability of the virus as a live-attenuated vaccine, reducing unwanted side effects. The MGF 110-11L gene was deleted using the CRISPR/Cas9 method, and the safety and efficacy of the virus were tested in pigs after isolation. The vaccine candidates administered at high doses showed reduced pathogenicity compared to the parental strain and induced immunity in vaccinated animals, although several mild clinical signs were observed. Although Lv17/WB/Rie1/d110-11L cannot be used as a vaccine in its current form, it was encouraging that the undesirable side effects of Lv17/WB/Rie1 at high doses can be reduced by additional mutations without a significant reduction in its protective capacity. MDPI 2023-04-14 /pmc/articles/PMC10145817/ /pubmed/37112759 http://dx.doi.org/10.3390/vaccines11040846 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tamás, Vivien Righi, Cecilia Mészáros, István D’Errico, Federica Olasz, Ferenc Casciari, Cristina Zádori, Zoltán Magyar, Tibor Petrini, Stefano Feliziani, Francesco Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence |
title | Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence |
title_full | Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence |
title_fullStr | Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence |
title_full_unstemmed | Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence |
title_short | Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence |
title_sort | involvement of the mgf 110-11l gene in the african swine fever replication and virulence |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145817/ https://www.ncbi.nlm.nih.gov/pubmed/37112759 http://dx.doi.org/10.3390/vaccines11040846 |
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