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Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure

Background and Objectives: Prostate cancer is on the rise in the European Union, and radiofrequency ablation (RFA) is one of the minimally invasive treatment options used for its treatment. Therefore, the aim of this study was to investigate and analyze the effects of RFA on prostate tissues. Materi...

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Autores principales: Skaudickas, Darijus, Vaitiekaitis, Gintautas, Liobikas, Julius, Gružienė, Aldona, Abras, Marcel, Gersone, Gita, Vitkus, Aleksandras, Kerzienė, Sigita, Undžytė, Greta, Veikutis, Vincentas, Kašauskas, Artūras, Baginskas, Armuntas, Noreika, Algis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145827/
https://www.ncbi.nlm.nih.gov/pubmed/37109660
http://dx.doi.org/10.3390/medicina59040702
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author Skaudickas, Darijus
Vaitiekaitis, Gintautas
Liobikas, Julius
Gružienė, Aldona
Abras, Marcel
Gersone, Gita
Vitkus, Aleksandras
Kerzienė, Sigita
Undžytė, Greta
Veikutis, Vincentas
Kašauskas, Artūras
Baginskas, Armuntas
Noreika, Algis
author_facet Skaudickas, Darijus
Vaitiekaitis, Gintautas
Liobikas, Julius
Gružienė, Aldona
Abras, Marcel
Gersone, Gita
Vitkus, Aleksandras
Kerzienė, Sigita
Undžytė, Greta
Veikutis, Vincentas
Kašauskas, Artūras
Baginskas, Armuntas
Noreika, Algis
author_sort Skaudickas, Darijus
collection PubMed
description Background and Objectives: Prostate cancer is on the rise in the European Union, and radiofrequency ablation (RFA) is one of the minimally invasive treatment options used for its treatment. Therefore, the aim of this study was to investigate and analyze the effects of RFA on prostate tissues. Materials and Methods: A standard prostate RFA procedure was performed on 13 non-purebred dogs in three sessions: no cooling (NC), cooling with a 0.1% NaCl solution (C.01), and cooling using a 0.9% NaCl solution (C.09). Microtome-cut 2–3 µm sections of prostate samples were stained with hematoxylin and eosin and further examined. Results: A histopathologic evaluation identified four zones of exposure: direct, application, necrosis, and transitional, as the damage on tissues decreased going further from the ablation site. The areas and perimeters of these zones were calculated, and geometric shapes of ablative lesions were evaluated using the quotient formula. Areas and perimeters of prostate tissue lesions in the NC and C.09 sessions were of similar size, whereas those found in C.01 were statistically significantly smaller. Lesions observed in session C.01 were of the most regular geometric shape, while the most irregular ones were found in session C.09. The shapes of lesions closest to the ablation electrode were the most irregular, becoming more regular the further away from the electrode they were. Conclusions: Prostate RFA leads to tissue damage with distinct morphological zones. Notably, the prostate lesions were the smallest and the most regular in shape after RFA procedures using the 0.1% NaCl cooling solution. It can be argued that smaller ablation sites may result in smaller scars, thus allowing for faster tissue healing if the blood flow and innervation at the ablation site are not compromised.
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spelling pubmed-101458272023-04-29 Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure Skaudickas, Darijus Vaitiekaitis, Gintautas Liobikas, Julius Gružienė, Aldona Abras, Marcel Gersone, Gita Vitkus, Aleksandras Kerzienė, Sigita Undžytė, Greta Veikutis, Vincentas Kašauskas, Artūras Baginskas, Armuntas Noreika, Algis Medicina (Kaunas) Article Background and Objectives: Prostate cancer is on the rise in the European Union, and radiofrequency ablation (RFA) is one of the minimally invasive treatment options used for its treatment. Therefore, the aim of this study was to investigate and analyze the effects of RFA on prostate tissues. Materials and Methods: A standard prostate RFA procedure was performed on 13 non-purebred dogs in three sessions: no cooling (NC), cooling with a 0.1% NaCl solution (C.01), and cooling using a 0.9% NaCl solution (C.09). Microtome-cut 2–3 µm sections of prostate samples were stained with hematoxylin and eosin and further examined. Results: A histopathologic evaluation identified four zones of exposure: direct, application, necrosis, and transitional, as the damage on tissues decreased going further from the ablation site. The areas and perimeters of these zones were calculated, and geometric shapes of ablative lesions were evaluated using the quotient formula. Areas and perimeters of prostate tissue lesions in the NC and C.09 sessions were of similar size, whereas those found in C.01 were statistically significantly smaller. Lesions observed in session C.01 were of the most regular geometric shape, while the most irregular ones were found in session C.09. The shapes of lesions closest to the ablation electrode were the most irregular, becoming more regular the further away from the electrode they were. Conclusions: Prostate RFA leads to tissue damage with distinct morphological zones. Notably, the prostate lesions were the smallest and the most regular in shape after RFA procedures using the 0.1% NaCl cooling solution. It can be argued that smaller ablation sites may result in smaller scars, thus allowing for faster tissue healing if the blood flow and innervation at the ablation site are not compromised. MDPI 2023-04-03 /pmc/articles/PMC10145827/ /pubmed/37109660 http://dx.doi.org/10.3390/medicina59040702 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Skaudickas, Darijus
Vaitiekaitis, Gintautas
Liobikas, Julius
Gružienė, Aldona
Abras, Marcel
Gersone, Gita
Vitkus, Aleksandras
Kerzienė, Sigita
Undžytė, Greta
Veikutis, Vincentas
Kašauskas, Artūras
Baginskas, Armuntas
Noreika, Algis
Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_full Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_fullStr Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_full_unstemmed Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_short Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_sort analysis of morphological and morphometric changes in a parenchymal tissue after the radiofrequency ablation procedure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145827/
https://www.ncbi.nlm.nih.gov/pubmed/37109660
http://dx.doi.org/10.3390/medicina59040702
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