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Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis

INTRODUCTION: β-Mannanase is a plant cell wall remodeling enzyme involved in the breakdown of hemicellulose and plays an important role in growth by hydrolyzing the mannan-like polysaccharide, but its function in adaptation to salt stress has been less studied. METHODS: Based on cloned the mannanase...

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Autores principales: Xu, Juanjuan, Yang, Caiyu, Ji, Shangyao, Ma, Hui, Lin, Jingwei, Li, Hui, Chen, Shuisen, Xu, Hai, Zhong, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145921/
https://www.ncbi.nlm.nih.gov/pubmed/37123825
http://dx.doi.org/10.3389/fpls.2023.1118548
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author Xu, Juanjuan
Yang, Caiyu
Ji, Shangyao
Ma, Hui
Lin, Jingwei
Li, Hui
Chen, Shuisen
Xu, Hai
Zhong, Ming
author_facet Xu, Juanjuan
Yang, Caiyu
Ji, Shangyao
Ma, Hui
Lin, Jingwei
Li, Hui
Chen, Shuisen
Xu, Hai
Zhong, Ming
author_sort Xu, Juanjuan
collection PubMed
description INTRODUCTION: β-Mannanase is a plant cell wall remodeling enzyme involved in the breakdown of hemicellulose and plays an important role in growth by hydrolyzing the mannan-like polysaccharide, but its function in adaptation to salt stress has been less studied. METHODS: Based on cloned the mannanase (MAN) gene from Mirabilis jalapa L., the study was carried out by heterologously expressing the gene in Arabidopsis thaliana, and then observing the plant phenotypes and measuring relevant physiological and biochemical indicators under 150 mM salt treatment. RESULTS AND DISCUSSION: The results indicate that MirMAN is a protein with a glycohydrolase-specific structural domain located in the cell wall. We first found that MirMAN reduced the susceptibility of transgenic Arabidopsis thaliana to high salt stress and increased the survival rate of plants by 38%. This was corroborated by the following significant changes, including the reduction in reactive oxygen species (ROS) levels, increase in antioxidant enzyme activity, accumulation of soluble sugars and increase of the expression level of RD29 in transgenic plants. We also found thatthe heterologous expression of MirMAN promoted root growth mainly by elongating the primary roots and increasing the density of lateral roots. Meanwhile, the expression of ARF7, ARF19, LBD16 and LBD29 was up-regulated in the transgenic plants, and the concentration of IAA in the roots was increased. Those results indicate that MirMAN is involved in the initiation of lateral root primordia in transgenic plants through the IAA-ARF signalling pathway. In conclusion, MirMAN improves plant salt tolerance not only by regulating ROS homeostasis, but also by promoting the development of lateral roots. Reflecting the potential of the MirMAN to promote root plastic development in adaptation to salt stress adversity.
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spelling pubmed-101459212023-04-29 Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis Xu, Juanjuan Yang, Caiyu Ji, Shangyao Ma, Hui Lin, Jingwei Li, Hui Chen, Shuisen Xu, Hai Zhong, Ming Front Plant Sci Plant Science INTRODUCTION: β-Mannanase is a plant cell wall remodeling enzyme involved in the breakdown of hemicellulose and plays an important role in growth by hydrolyzing the mannan-like polysaccharide, but its function in adaptation to salt stress has been less studied. METHODS: Based on cloned the mannanase (MAN) gene from Mirabilis jalapa L., the study was carried out by heterologously expressing the gene in Arabidopsis thaliana, and then observing the plant phenotypes and measuring relevant physiological and biochemical indicators under 150 mM salt treatment. RESULTS AND DISCUSSION: The results indicate that MirMAN is a protein with a glycohydrolase-specific structural domain located in the cell wall. We first found that MirMAN reduced the susceptibility of transgenic Arabidopsis thaliana to high salt stress and increased the survival rate of plants by 38%. This was corroborated by the following significant changes, including the reduction in reactive oxygen species (ROS) levels, increase in antioxidant enzyme activity, accumulation of soluble sugars and increase of the expression level of RD29 in transgenic plants. We also found thatthe heterologous expression of MirMAN promoted root growth mainly by elongating the primary roots and increasing the density of lateral roots. Meanwhile, the expression of ARF7, ARF19, LBD16 and LBD29 was up-regulated in the transgenic plants, and the concentration of IAA in the roots was increased. Those results indicate that MirMAN is involved in the initiation of lateral root primordia in transgenic plants through the IAA-ARF signalling pathway. In conclusion, MirMAN improves plant salt tolerance not only by regulating ROS homeostasis, but also by promoting the development of lateral roots. Reflecting the potential of the MirMAN to promote root plastic development in adaptation to salt stress adversity. Frontiers Media S.A. 2023-04-14 /pmc/articles/PMC10145921/ /pubmed/37123825 http://dx.doi.org/10.3389/fpls.2023.1118548 Text en Copyright © 2023 Xu, Yang, Ji, Ma, Lin, Li, Chen, Xu and Zhong https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xu, Juanjuan
Yang, Caiyu
Ji, Shangyao
Ma, Hui
Lin, Jingwei
Li, Hui
Chen, Shuisen
Xu, Hai
Zhong, Ming
Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis
title Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis
title_full Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis
title_fullStr Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis
title_full_unstemmed Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis
title_short Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis
title_sort heterologous expression of mirman enhances root development and salt tolerance in arabidopsis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10145921/
https://www.ncbi.nlm.nih.gov/pubmed/37123825
http://dx.doi.org/10.3389/fpls.2023.1118548
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