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Virulence Profiles of Wild-Type, P.1 and Delta SARS-CoV-2 Variants in K18-hACE2 Transgenic Mice

Since December 2019, the world has been experiencing the COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and we now face the emergence of several variants. We aimed to assess the differences between the wild-type (Wt) (Wuhan) strain and the P.1 (Gamma) a...

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Detalles Bibliográficos
Autores principales: da Silva Santos, Yasmin, Gamon, Thais Helena Martins, de Azevedo, Marcela Santiago Pacheco, Telezynski, Bruna Larotonda, de Souza, Edmarcia Elisa, de Oliveira, Danielle Bruna Leal, Dombrowski, Jamille Gregório, Rosa-Fernandes, Livia, Palmisano, Giuseppe, de Moura Carvalho, Leonardo José, Luvizotto, Maria Cecília Rui, Wrenger, Carsten, Covas, Dimas Tadeu, Curi, Rui, Marinho, Claudio Romero Farias, Durigon, Edison Luiz, Epiphanio, Sabrina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146242/
https://www.ncbi.nlm.nih.gov/pubmed/37112979
http://dx.doi.org/10.3390/v15040999
Descripción
Sumario:Since December 2019, the world has been experiencing the COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and we now face the emergence of several variants. We aimed to assess the differences between the wild-type (Wt) (Wuhan) strain and the P.1 (Gamma) and Delta variants using infected K18-hACE2 mice. The clinical manifestations, behavior, virus load, pulmonary capacity, and histopathological alterations were analyzed. The P.1-infected mice showed weight loss and more severe clinical manifestations of COVID-19 than the Wt and Delta-infected mice. The respiratory capacity was reduced in the P.1-infected mice compared to the other groups. Pulmonary histological findings demonstrated that a more aggressive disease was generated by the P.1 and Delta variants compared to the Wt strain of the virus. The quantification of the SARS-CoV-2 viral copies varied greatly among the infected mice although it was higher in P.1-infected mice on the day of death. Our data revealed that K18-hACE2 mice infected with the P.1 variant develop a more severe infectious disease than those infected with the other variants, despite the significant heterogeneity among the mice.