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The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions
The mucociliary airway epithelium lines the human airways and is the primary site of host-environmental interactions in the lung. Following virus infection, airway epithelial cells initiate an innate immune response to suppress virus replication. Therefore, defining the virus-host interactions of th...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146425/ https://www.ncbi.nlm.nih.gov/pubmed/37112842 http://dx.doi.org/10.3390/v15040862 |
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author | Subramaniyan, Bharathiraja Gurung, Sunam Bodas, Manish Moore, Andrew R. Larabee, Jason L. Reuter, Darlene Georgescu, Constantin Wren, Jonathan D. Myers, Dean A. Papin, James F. Walters, Matthew S. |
author_facet | Subramaniyan, Bharathiraja Gurung, Sunam Bodas, Manish Moore, Andrew R. Larabee, Jason L. Reuter, Darlene Georgescu, Constantin Wren, Jonathan D. Myers, Dean A. Papin, James F. Walters, Matthew S. |
author_sort | Subramaniyan, Bharathiraja |
collection | PubMed |
description | The mucociliary airway epithelium lines the human airways and is the primary site of host-environmental interactions in the lung. Following virus infection, airway epithelial cells initiate an innate immune response to suppress virus replication. Therefore, defining the virus-host interactions of the mucociliary airway epithelium is critical for understanding the mechanisms that regulate virus infection, including Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Non-human primates (NHP) are closely related to humans and provide a model to study human disease. However, ethical considerations and high costs can restrict the use of in vivo NHP models. Therefore, there is a need to develop in vitro NHP models of human respiratory virus infection that would allow for rapidly characterizing virus tropism and the suitability of specific NHP species to model human infection. Using the olive baboon (Papio anubis), we have developed methodologies for the isolation, in vitro expansion, cryopreservation, and mucociliary differentiation of primary fetal baboon tracheal epithelial cells (FBTECs). Furthermore, we demonstrate that in vitro differentiated FBTECs are permissive to SARS-CoV-2 infection and produce a potent host innate-immune response. In summary, we have developed an in vitro NHP model that provides a platform for the study of SARS-CoV-2 infection and other human respiratory viruses. |
format | Online Article Text |
id | pubmed-10146425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101464252023-04-29 The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions Subramaniyan, Bharathiraja Gurung, Sunam Bodas, Manish Moore, Andrew R. Larabee, Jason L. Reuter, Darlene Georgescu, Constantin Wren, Jonathan D. Myers, Dean A. Papin, James F. Walters, Matthew S. Viruses Article The mucociliary airway epithelium lines the human airways and is the primary site of host-environmental interactions in the lung. Following virus infection, airway epithelial cells initiate an innate immune response to suppress virus replication. Therefore, defining the virus-host interactions of the mucociliary airway epithelium is critical for understanding the mechanisms that regulate virus infection, including Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Non-human primates (NHP) are closely related to humans and provide a model to study human disease. However, ethical considerations and high costs can restrict the use of in vivo NHP models. Therefore, there is a need to develop in vitro NHP models of human respiratory virus infection that would allow for rapidly characterizing virus tropism and the suitability of specific NHP species to model human infection. Using the olive baboon (Papio anubis), we have developed methodologies for the isolation, in vitro expansion, cryopreservation, and mucociliary differentiation of primary fetal baboon tracheal epithelial cells (FBTECs). Furthermore, we demonstrate that in vitro differentiated FBTECs are permissive to SARS-CoV-2 infection and produce a potent host innate-immune response. In summary, we have developed an in vitro NHP model that provides a platform for the study of SARS-CoV-2 infection and other human respiratory viruses. MDPI 2023-03-28 /pmc/articles/PMC10146425/ /pubmed/37112842 http://dx.doi.org/10.3390/v15040862 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Subramaniyan, Bharathiraja Gurung, Sunam Bodas, Manish Moore, Andrew R. Larabee, Jason L. Reuter, Darlene Georgescu, Constantin Wren, Jonathan D. Myers, Dean A. Papin, James F. Walters, Matthew S. The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions |
title | The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions |
title_full | The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions |
title_fullStr | The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions |
title_full_unstemmed | The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions |
title_short | The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions |
title_sort | isolation and in vitro differentiation of primary fetal baboon tracheal epithelial cells for the study of sars-cov-2 host-virus interactions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146425/ https://www.ncbi.nlm.nih.gov/pubmed/37112842 http://dx.doi.org/10.3390/v15040862 |
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