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Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration

Aromatic plants are reported to display pharmacological properties, including anti-aging. This work aims to disclose the anti-aging effect of the essential oil (EO) of Thymbra capitata (L.) Cav., an aromatic and medicinal plant widely used as a spice, as well as of the hydrodistillation residual wat...

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Autores principales: Alves-Silva, Jorge M., Pedreiro, Sónia, Cavaleiro, Carlos, Cruz, Maria Teresa, Figueirinha, Artur, Salgueiro, Lígia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146686/
https://www.ncbi.nlm.nih.gov/pubmed/37111149
http://dx.doi.org/10.3390/nu15081930
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author Alves-Silva, Jorge M.
Pedreiro, Sónia
Cavaleiro, Carlos
Cruz, Maria Teresa
Figueirinha, Artur
Salgueiro, Lígia
author_facet Alves-Silva, Jorge M.
Pedreiro, Sónia
Cavaleiro, Carlos
Cruz, Maria Teresa
Figueirinha, Artur
Salgueiro, Lígia
author_sort Alves-Silva, Jorge M.
collection PubMed
description Aromatic plants are reported to display pharmacological properties, including anti-aging. This work aims to disclose the anti-aging effect of the essential oil (EO) of Thymbra capitata (L.) Cav., an aromatic and medicinal plant widely used as a spice, as well as of the hydrodistillation residual water (HRW), a discarded by-product of EO hydrodistillation. The phytochemical characterization of EO and HRW was assessed by GC-MS and HPLC-PDA-ESI-MS(n), respectively. The DPPH, ABTS, and FRAP assays were used to disclose the antioxidant properties. The anti-inflammatory potential was evaluated using lipopolysaccharide-stimulated macrophages by assessing NO production, iNOS, and pro-IL-1β protein levels. Cell migration was evaluated using the scratch wound assay, and the etoposide-induced senescence was used to assess the modulation of senescence. The EO is mainly characterized by carvacrol, while the HRW is predominantly characterized by rosmarinic acid. The HRW exerts a stronger antioxidant effect in the DPPH and FRAP assays, whereas the EO was the most active sample in the ABTS assay. Both extracts reduce NO, iNOS, and pro-IL-1β. The EO has no effect on cell migration and presents anti-senescence effects. In opposition, HRW reduces cell migration and induces cellular senescence. Overall, our study highlights interesting pharmacological properties for both extracts, EO being of interest as an anti-aging ingredient and HRW relevant in cancer therapy.
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spelling pubmed-101466862023-04-29 Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration Alves-Silva, Jorge M. Pedreiro, Sónia Cavaleiro, Carlos Cruz, Maria Teresa Figueirinha, Artur Salgueiro, Lígia Nutrients Article Aromatic plants are reported to display pharmacological properties, including anti-aging. This work aims to disclose the anti-aging effect of the essential oil (EO) of Thymbra capitata (L.) Cav., an aromatic and medicinal plant widely used as a spice, as well as of the hydrodistillation residual water (HRW), a discarded by-product of EO hydrodistillation. The phytochemical characterization of EO and HRW was assessed by GC-MS and HPLC-PDA-ESI-MS(n), respectively. The DPPH, ABTS, and FRAP assays were used to disclose the antioxidant properties. The anti-inflammatory potential was evaluated using lipopolysaccharide-stimulated macrophages by assessing NO production, iNOS, and pro-IL-1β protein levels. Cell migration was evaluated using the scratch wound assay, and the etoposide-induced senescence was used to assess the modulation of senescence. The EO is mainly characterized by carvacrol, while the HRW is predominantly characterized by rosmarinic acid. The HRW exerts a stronger antioxidant effect in the DPPH and FRAP assays, whereas the EO was the most active sample in the ABTS assay. Both extracts reduce NO, iNOS, and pro-IL-1β. The EO has no effect on cell migration and presents anti-senescence effects. In opposition, HRW reduces cell migration and induces cellular senescence. Overall, our study highlights interesting pharmacological properties for both extracts, EO being of interest as an anti-aging ingredient and HRW relevant in cancer therapy. MDPI 2023-04-17 /pmc/articles/PMC10146686/ /pubmed/37111149 http://dx.doi.org/10.3390/nu15081930 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alves-Silva, Jorge M.
Pedreiro, Sónia
Cavaleiro, Carlos
Cruz, Maria Teresa
Figueirinha, Artur
Salgueiro, Lígia
Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration
title Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration
title_full Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration
title_fullStr Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration
title_full_unstemmed Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration
title_short Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration
title_sort effect of thymbra capitata (l.) cav. on inflammation, senescence and cell migration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146686/
https://www.ncbi.nlm.nih.gov/pubmed/37111149
http://dx.doi.org/10.3390/nu15081930
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