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Quantification of Blood Viscoelasticity under Microcapillary Blood Flow
Blood elasticity is quantified using a single compliance model by analyzing pulsatile blood flow. However, one compliance coefficient is influenced substantially by the microfluidic system (i.e., soft microfluidic channels and flexible tubing). The novelty of the present method comes from the assess...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146691/ https://www.ncbi.nlm.nih.gov/pubmed/37421047 http://dx.doi.org/10.3390/mi14040814 |
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author | Kang, Yang Jun |
author_facet | Kang, Yang Jun |
author_sort | Kang, Yang Jun |
collection | PubMed |
description | Blood elasticity is quantified using a single compliance model by analyzing pulsatile blood flow. However, one compliance coefficient is influenced substantially by the microfluidic system (i.e., soft microfluidic channels and flexible tubing). The novelty of the present method comes from the assessment of two distinct compliance coefficients, one for the sample and one for the microfluidic system. With two compliance coefficients, the viscoelasticity measurement can be disentangled from the influence of the measurement device. In this study, a coflowing microfluidic channel was used to estimate blood viscoelasticity. Two compliance coefficients were suggested to denote the effects of the polydimethylsiloxane (PDMS) channel and flexible tubing (C(1)), as well as those of the RBC (red blood cell) elasticity (C(2)), in a microfluidic system. On the basis of the fluidic circuit modeling technique, a governing equation for the interface in the coflowing was derived, and its analytical solution was obtained by solving the second-order differential equation. Using the analytic solution, two compliance coefficients were obtained via a nonlinear curve fitting technique. According to the experimental results, C(2)/C(1) is estimated to be approximately 10.9–20.4 with respect to channel depth (h = 4, 10, and 20 µm). The PDMS channel depth contributed simultaneously to the increase in the two compliance coefficients, whereas the outlet tubing caused a decrease in C(1). The two compliance coefficients and blood viscosity varied substantially with respect to homogeneous hardened RBCs or heterogeneous hardened RBCs. In conclusion, the proposed method can be used to effectively detect changes in blood or microfluidic systems. In future studies, the present method can contribute to the detection of subpopulations of RBCs in the patient’s blood. |
format | Online Article Text |
id | pubmed-10146691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101466912023-04-29 Quantification of Blood Viscoelasticity under Microcapillary Blood Flow Kang, Yang Jun Micromachines (Basel) Article Blood elasticity is quantified using a single compliance model by analyzing pulsatile blood flow. However, one compliance coefficient is influenced substantially by the microfluidic system (i.e., soft microfluidic channels and flexible tubing). The novelty of the present method comes from the assessment of two distinct compliance coefficients, one for the sample and one for the microfluidic system. With two compliance coefficients, the viscoelasticity measurement can be disentangled from the influence of the measurement device. In this study, a coflowing microfluidic channel was used to estimate blood viscoelasticity. Two compliance coefficients were suggested to denote the effects of the polydimethylsiloxane (PDMS) channel and flexible tubing (C(1)), as well as those of the RBC (red blood cell) elasticity (C(2)), in a microfluidic system. On the basis of the fluidic circuit modeling technique, a governing equation for the interface in the coflowing was derived, and its analytical solution was obtained by solving the second-order differential equation. Using the analytic solution, two compliance coefficients were obtained via a nonlinear curve fitting technique. According to the experimental results, C(2)/C(1) is estimated to be approximately 10.9–20.4 with respect to channel depth (h = 4, 10, and 20 µm). The PDMS channel depth contributed simultaneously to the increase in the two compliance coefficients, whereas the outlet tubing caused a decrease in C(1). The two compliance coefficients and blood viscosity varied substantially with respect to homogeneous hardened RBCs or heterogeneous hardened RBCs. In conclusion, the proposed method can be used to effectively detect changes in blood or microfluidic systems. In future studies, the present method can contribute to the detection of subpopulations of RBCs in the patient’s blood. MDPI 2023-04-03 /pmc/articles/PMC10146691/ /pubmed/37421047 http://dx.doi.org/10.3390/mi14040814 Text en © 2023 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kang, Yang Jun Quantification of Blood Viscoelasticity under Microcapillary Blood Flow |
title | Quantification of Blood Viscoelasticity under Microcapillary Blood Flow |
title_full | Quantification of Blood Viscoelasticity under Microcapillary Blood Flow |
title_fullStr | Quantification of Blood Viscoelasticity under Microcapillary Blood Flow |
title_full_unstemmed | Quantification of Blood Viscoelasticity under Microcapillary Blood Flow |
title_short | Quantification of Blood Viscoelasticity under Microcapillary Blood Flow |
title_sort | quantification of blood viscoelasticity under microcapillary blood flow |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146691/ https://www.ncbi.nlm.nih.gov/pubmed/37421047 http://dx.doi.org/10.3390/mi14040814 |
work_keys_str_mv | AT kangyangjun quantificationofbloodviscoelasticityundermicrocapillarybloodflow |