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Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics

An early and accurate diagnosis of Candida albicans is critical for the rapid antifungal treatment of candidemia, a mortal bloodstream infection. This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the bloo...

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Autores principales: Lim, Hyunjung, Kim, Jae Young, Choo, Seunghee, Lee, Changseok, Han, Byoung Joe, Lim, Chae Seung, Nam, Jeonghun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146744/
https://www.ncbi.nlm.nih.gov/pubmed/37420947
http://dx.doi.org/10.3390/mi14040712
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author Lim, Hyunjung
Kim, Jae Young
Choo, Seunghee
Lee, Changseok
Han, Byoung Joe
Lim, Chae Seung
Nam, Jeonghun
author_facet Lim, Hyunjung
Kim, Jae Young
Choo, Seunghee
Lee, Changseok
Han, Byoung Joe
Lim, Chae Seung
Nam, Jeonghun
author_sort Lim, Hyunjung
collection PubMed
description An early and accurate diagnosis of Candida albicans is critical for the rapid antifungal treatment of candidemia, a mortal bloodstream infection. This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the blood. The total sample preparation system contains two-step microfluidic devices: a closed-loop separation and concentration device and a co-flow cell-washing device. To determine the flow conditions of the closed-loop device, such as the flow rate factor, a mixture of 4 and 13 μm particles was used. Candida cells were successfully separated from the white blood cells (WBCs) and concentrated by 74.6-fold in the sample reservoir of the closed-loop system at 800 μL/min with a flow rate factor of 3.3. In addition, the collected Candida cells were washed with washing buffer (deionized water) in the microchannels with an aspect ratio of 2 at a total flow rate of 100 μL/min. Finally, Candida cells at extremely low concentrations (Ct > 35) became detectable after the removal of WBCs, the additional buffer solution in the closed-loop system (Ct = 30.3 ± 1.3), and further removal of blood lysate and washing (Ct = 23.3 ± 1.6).
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spelling pubmed-101467442023-04-29 Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics Lim, Hyunjung Kim, Jae Young Choo, Seunghee Lee, Changseok Han, Byoung Joe Lim, Chae Seung Nam, Jeonghun Micromachines (Basel) Article An early and accurate diagnosis of Candida albicans is critical for the rapid antifungal treatment of candidemia, a mortal bloodstream infection. This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the blood. The total sample preparation system contains two-step microfluidic devices: a closed-loop separation and concentration device and a co-flow cell-washing device. To determine the flow conditions of the closed-loop device, such as the flow rate factor, a mixture of 4 and 13 μm particles was used. Candida cells were successfully separated from the white blood cells (WBCs) and concentrated by 74.6-fold in the sample reservoir of the closed-loop system at 800 μL/min with a flow rate factor of 3.3. In addition, the collected Candida cells were washed with washing buffer (deionized water) in the microchannels with an aspect ratio of 2 at a total flow rate of 100 μL/min. Finally, Candida cells at extremely low concentrations (Ct > 35) became detectable after the removal of WBCs, the additional buffer solution in the closed-loop system (Ct = 30.3 ± 1.3), and further removal of blood lysate and washing (Ct = 23.3 ± 1.6). MDPI 2023-03-23 /pmc/articles/PMC10146744/ /pubmed/37420947 http://dx.doi.org/10.3390/mi14040712 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lim, Hyunjung
Kim, Jae Young
Choo, Seunghee
Lee, Changseok
Han, Byoung Joe
Lim, Chae Seung
Nam, Jeonghun
Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics
title Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics
title_full Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics
title_fullStr Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics
title_full_unstemmed Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics
title_short Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics
title_sort separation and washing of candida cells from white blood cells using viscoelastic microfluidics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146744/
https://www.ncbi.nlm.nih.gov/pubmed/37420947
http://dx.doi.org/10.3390/mi14040712
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