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Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers

Francisella tularensis is a zoonotic bacterium that is endemic in large parts of the world. It is absent in the standard library of the most applied matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems: the Vitek MS and the Bruker Biotyper system. The a...

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Autores principales: de Vries, Maaike C., Hoeve-Bakker, B. J. A., van den Beld, Maaike J. C., Hendriks, Amber C. A., Harpal, Airien S. D., Noomen, Ramón C. E. A., Reubsaet, Frans A. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146885/
https://www.ncbi.nlm.nih.gov/pubmed/37110328
http://dx.doi.org/10.3390/microorganisms11040905
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author de Vries, Maaike C.
Hoeve-Bakker, B. J. A.
van den Beld, Maaike J. C.
Hendriks, Amber C. A.
Harpal, Airien S. D.
Noomen, Ramón C. E. A.
Reubsaet, Frans A. G.
author_facet de Vries, Maaike C.
Hoeve-Bakker, B. J. A.
van den Beld, Maaike J. C.
Hendriks, Amber C. A.
Harpal, Airien S. D.
Noomen, Ramón C. E. A.
Reubsaet, Frans A. G.
author_sort de Vries, Maaike C.
collection PubMed
description Francisella tularensis is a zoonotic bacterium that is endemic in large parts of the world. It is absent in the standard library of the most applied matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems: the Vitek MS and the Bruker Biotyper system. The additional Bruker MALDI Biotyper Security library contains F. tularensis without subspecies differentiation. The virulence of F. tularensis differs between the subspecies. The F. tularensis subspecies (ssp.) tularensis is highly pathogenic, whereas the subspecies holarctica displays lower virulence and subspecies novicida and F. tularensis ssp. mediasiatica are hardly virulent. To differentiate the Francisellaceae and the F. tularensis-subspecies, an in-house Francisella library was built with the Bruker Biotyper system and validated together with the existing Bruker databases. In addition, specific biomarkers were defined based on the main spectra of the Francisella strains supplemented with in silico genome data. Our in-house Francisella library accurately differentiates the F. tularensis subspecies and the other Francisellaceae. The biomarkers correctly differentiate the various species within the genus Francisella and the F. tularensis subspecies. These MALDI-TOF MS strategies can successfully be applied in a clinical laboratory setting as a fast and specific method to identify F. tularensis to subspecies level.
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spelling pubmed-101468852023-04-29 Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers de Vries, Maaike C. Hoeve-Bakker, B. J. A. van den Beld, Maaike J. C. Hendriks, Amber C. A. Harpal, Airien S. D. Noomen, Ramón C. E. A. Reubsaet, Frans A. G. Microorganisms Article Francisella tularensis is a zoonotic bacterium that is endemic in large parts of the world. It is absent in the standard library of the most applied matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems: the Vitek MS and the Bruker Biotyper system. The additional Bruker MALDI Biotyper Security library contains F. tularensis without subspecies differentiation. The virulence of F. tularensis differs between the subspecies. The F. tularensis subspecies (ssp.) tularensis is highly pathogenic, whereas the subspecies holarctica displays lower virulence and subspecies novicida and F. tularensis ssp. mediasiatica are hardly virulent. To differentiate the Francisellaceae and the F. tularensis-subspecies, an in-house Francisella library was built with the Bruker Biotyper system and validated together with the existing Bruker databases. In addition, specific biomarkers were defined based on the main spectra of the Francisella strains supplemented with in silico genome data. Our in-house Francisella library accurately differentiates the F. tularensis subspecies and the other Francisellaceae. The biomarkers correctly differentiate the various species within the genus Francisella and the F. tularensis subspecies. These MALDI-TOF MS strategies can successfully be applied in a clinical laboratory setting as a fast and specific method to identify F. tularensis to subspecies level. MDPI 2023-03-30 /pmc/articles/PMC10146885/ /pubmed/37110328 http://dx.doi.org/10.3390/microorganisms11040905 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Vries, Maaike C.
Hoeve-Bakker, B. J. A.
van den Beld, Maaike J. C.
Hendriks, Amber C. A.
Harpal, Airien S. D.
Noomen, Ramón C. E. A.
Reubsaet, Frans A. G.
Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers
title Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers
title_full Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers
title_fullStr Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers
title_full_unstemmed Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers
title_short Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers
title_sort identification of francisella tularensis subspecies in a clinical setting using maldi-tof ms: an in-house francisella library and biomarkers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146885/
https://www.ncbi.nlm.nih.gov/pubmed/37110328
http://dx.doi.org/10.3390/microorganisms11040905
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