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Esophageal mycobiome landscape and interkingdom interactions in esophageal squamous cell carcinoma

BACKGROUND: The study purpose was to characterize the mycobiome and its associations with the expression of pathogenic genes in esophageal squamous cell carcinoma (ESCC). METHODS: Patients with primary ESCC were recruited from two central hospitals. We performed internal transcribed spacer 1 (ITS1)...

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Autores principales: Rao, Wen-Qing, Lin, Zheng, Jiang, Jian, Wang, Jian-Wen, Lin, Zhi-Feng, Fu, Rong, Chen, Wei-Lin, Chen, Yuan-Mei, Peng, Xian-E, Hu, Zhi-Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10147516/
https://www.ncbi.nlm.nih.gov/pubmed/37124071
http://dx.doi.org/10.1093/gastro/goad022
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author Rao, Wen-Qing
Lin, Zheng
Jiang, Jian
Wang, Jian-Wen
Lin, Zhi-Feng
Fu, Rong
Chen, Wei-Lin
Chen, Yuan-Mei
Peng, Xian-E
Hu, Zhi-Jian
author_facet Rao, Wen-Qing
Lin, Zheng
Jiang, Jian
Wang, Jian-Wen
Lin, Zhi-Feng
Fu, Rong
Chen, Wei-Lin
Chen, Yuan-Mei
Peng, Xian-E
Hu, Zhi-Jian
author_sort Rao, Wen-Qing
collection PubMed
description BACKGROUND: The study purpose was to characterize the mycobiome and its associations with the expression of pathogenic genes in esophageal squamous cell carcinoma (ESCC). METHODS: Patients with primary ESCC were recruited from two central hospitals. We performed internal transcribed spacer 1 (ITS1) ribosomal DNA sequencing analysis. We compared differential fungi and explored the ecology of fungi and the interaction of bacteria and fungi. RESULTS: The mycobiota diversity was significantly different between tumors and tumor-adjacent samples. We further analysed the differences between the two groups, at the species level, confirming that Rhodotorula toruloides, Malassezia dermatis, Hanseniaspora lachancei, and Spegazzinia tessarthra were excessively colonized in the tumor samples, whereas Preussia persica, Fusarium solani, Nigrospora oryzae, Acremonium furcatum, Golovinomyces artemisiae, and Tausonia pullulans were significantly more abundant in tumor-adjacent samples. The fungal co-occurrence network in tumor-adjacent samples was larger and denser than that in tumors. Similarly, the more complex bacterial–fungal interactions in tumor-adjacent samples were also detected. The expression of mechanistic target of rapamycin kinase was positively correlated with the abundance of N. oryzae and T. pullulans in tumor-adjacent samples. In tumors, the expression of MET proto-oncogene, receptor tyrosine kinase (MET) had a negative correlation and a positive correlation with the abundance of R. toruloides and S. tessarthra, respectively. CONCLUSION: This study revealed the landscape of the esophageal mycobiome characterized by an altered fungal composition and bacterial and fungal ecology in ESCC.
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spelling pubmed-101475162023-04-29 Esophageal mycobiome landscape and interkingdom interactions in esophageal squamous cell carcinoma Rao, Wen-Qing Lin, Zheng Jiang, Jian Wang, Jian-Wen Lin, Zhi-Feng Fu, Rong Chen, Wei-Lin Chen, Yuan-Mei Peng, Xian-E Hu, Zhi-Jian Gastroenterol Rep (Oxf) Original Article BACKGROUND: The study purpose was to characterize the mycobiome and its associations with the expression of pathogenic genes in esophageal squamous cell carcinoma (ESCC). METHODS: Patients with primary ESCC were recruited from two central hospitals. We performed internal transcribed spacer 1 (ITS1) ribosomal DNA sequencing analysis. We compared differential fungi and explored the ecology of fungi and the interaction of bacteria and fungi. RESULTS: The mycobiota diversity was significantly different between tumors and tumor-adjacent samples. We further analysed the differences between the two groups, at the species level, confirming that Rhodotorula toruloides, Malassezia dermatis, Hanseniaspora lachancei, and Spegazzinia tessarthra were excessively colonized in the tumor samples, whereas Preussia persica, Fusarium solani, Nigrospora oryzae, Acremonium furcatum, Golovinomyces artemisiae, and Tausonia pullulans were significantly more abundant in tumor-adjacent samples. The fungal co-occurrence network in tumor-adjacent samples was larger and denser than that in tumors. Similarly, the more complex bacterial–fungal interactions in tumor-adjacent samples were also detected. The expression of mechanistic target of rapamycin kinase was positively correlated with the abundance of N. oryzae and T. pullulans in tumor-adjacent samples. In tumors, the expression of MET proto-oncogene, receptor tyrosine kinase (MET) had a negative correlation and a positive correlation with the abundance of R. toruloides and S. tessarthra, respectively. CONCLUSION: This study revealed the landscape of the esophageal mycobiome characterized by an altered fungal composition and bacterial and fungal ecology in ESCC. Oxford University Press 2023-04-28 /pmc/articles/PMC10147516/ /pubmed/37124071 http://dx.doi.org/10.1093/gastro/goad022 Text en © The Author(s) 2023. Published by Oxford University Press and Sixth Affiliated Hospital of Sun Yat-sen University https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Original Article
Rao, Wen-Qing
Lin, Zheng
Jiang, Jian
Wang, Jian-Wen
Lin, Zhi-Feng
Fu, Rong
Chen, Wei-Lin
Chen, Yuan-Mei
Peng, Xian-E
Hu, Zhi-Jian
Esophageal mycobiome landscape and interkingdom interactions in esophageal squamous cell carcinoma
title Esophageal mycobiome landscape and interkingdom interactions in esophageal squamous cell carcinoma
title_full Esophageal mycobiome landscape and interkingdom interactions in esophageal squamous cell carcinoma
title_fullStr Esophageal mycobiome landscape and interkingdom interactions in esophageal squamous cell carcinoma
title_full_unstemmed Esophageal mycobiome landscape and interkingdom interactions in esophageal squamous cell carcinoma
title_short Esophageal mycobiome landscape and interkingdom interactions in esophageal squamous cell carcinoma
title_sort esophageal mycobiome landscape and interkingdom interactions in esophageal squamous cell carcinoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10147516/
https://www.ncbi.nlm.nih.gov/pubmed/37124071
http://dx.doi.org/10.1093/gastro/goad022
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